In vivo distribution and expression after skin injury of fibronectin splicing variants

Abstract

Abstract: Objective In order to develop new criteria and to select positive controls for forensic medicine, experiments on in vivo distribution and expression after skin wound of fibronectin splicing variants, EⅢA and EⅢB were studied. Methods Rat tissues were homogenized to extract total RNA which was then amplified by RT-PCR technique. Results (1)EⅢA+ and EⅢB+ of normal skin were not expressed, whereas EⅢA+ after 18 hours' injury was expressed abundantly. No obvious charge of EⅢB+ was detected. The differences between results of using two methods of injury were not significant.(2)The distribution of fibronectin variants in different adult organs were in the following order: brain >heart >liver. Conclusion EⅢA+ may be used as a sensitive criterion for research of skin injury.

Key words: skin injury, fibronectin, alternative splicing

CHEN YI-JIU;LIU NING-GUO;HUANG XIAO-HUA;ET AL (THE INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;SHANGHAI 000). In vivo distribution and expression after skin injury of fibronectin splicing variants[J]. , 2001, 0(4): 193-195.

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In vivo distribution and expression after skin injury of fibronectin splicing variants

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