Expression of EⅢA-fibronectin in injured rat skin used in estimation of wound interval

Abstract

Abstract: Objective To observe the means of fibronectin(FN)alternative splicing and the expression of E Ⅲ A-FN variant in rat skin after bruise,for the sake of providing some help for forensic estimation of wound interval.Methods Total RNA was isolated from wounded skin,and reverse transcription polymerase chain reaction was conducted to amplify target segments.Results Detectable E Ⅲ A + (526bp)segments,lacked in normal organize,was amplified at1h after experimental wound,and the levels were increased within24h.Conclusion The alter native splicing E Ⅲ A-fibronectin variant would be a satisfied criterion for research of skin injury.

Key words: fibronectin, alternative splicing, E Ⅲ A, wound interval estimation

LIU NING-GUO;CHEN YI-JIU;HUANG XIAO-HUA;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI000). Expression of EⅢA-fibronectin in injured rat skin used in estimation of wound interval[J]. , 2002, 0(3): 129-131.

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Expression of EⅢA-fibronectin in injured rat skin used in estimation of wound interval

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