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28 June 2002, Volume 18 Issue 3 Previous Issue    Next Issue
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Expression of EⅢA-fibronectin in injured rat skin used in estimation of wound interval LIU NING-GUO;CHEN YI-JIU;HUANG XIAO-HUA;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI000) 2002, 0(3): 129-131.  Abstract ( 2244 )   Objective To observe the means of fibronectin(FN)alternative splicing and the expression of E Ⅲ A-FN variant in rat skin after bruise,for the sake of providing some help for forensic estimation of wound interval.Methods Total RNA was isolated from wounded skin,and reverse transcription polymerase chain reaction was conducted to amplify target segments.Results Detectable E Ⅲ A + (526bp)segments,lacked in normal organize,was amplified at1h after experimental wound,and the levels were increased within24h.Conclusion The alter native splicing E Ⅲ A-fibronectin variant would be a satisfied criterion for research of skin injury. Related Articles | Metrics

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Tryptase and fatal anaphylaxic reaction SHEN YI-WEN;LU CHAO;ZHAO ZI-QIN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000) 2002, 0(3): 132-134+.  Abstract ( 2184 )   Objective To investigate the relationship between tryptase in serum and anaphylaxis.Methods The concentrations of tryptase in the sera of heart blood in three persons died from anaphylaxis shock were detected by ELISA.The first sample was obtained from a man,aged38,died of injecting Amikacin.The second sample was obtained from a man,aged42,died of injecting Cephradine.The third sample was from a woman,aged39,died of injecting Lincomycin.All samples were stored in-20℃.Results The concentrations of tryptase in sera were52ng/ml,121ng/ml and0.73ng/ml.It was unknown why the concentration of tryptase in the third sample was normal.Conclution In fetal anaphylaxia reaction tryptase measurement is a useful indicator,but the diagnosis is not to be based on the test alone. Related Articles | Metrics

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A preliminary study of epidemiology of sudden manhood death syndrome in the peasant derived workers in Dongguan city CHENG JIAN-DING ;CHEN YU-CHUAN ;ZENG JIA-LE ;ET AL.(.DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU000;.THE BUREAU OF THE PUBLIC SECURITY OF DONGGUAN CITY;DONGGUAN;GUANGDONG PROVINCE0 2002, 0(3): 135-136.  Abstract ( 2111 )   Objective To study the feature of sudden manhood death syndrome(SMDS)in Dongguan city.Methods The data of284cases of SMDS were analysized by retrospective study.Results The distri bution of age,sex,the time of death and hometown of the dead in SMDS were described.The clinical or anatomical characters of SMDS were also discussed.Conclusion These data will contribute to the late epi demical study. Related Articles | Metrics

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Apoptosis in mouse after tetramine poisoning CHENG YI-BIN;LIU NING-GUO;ZHANG JIAN-HUA;ET AL(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI000) 2002, 0(3): 137-139+.  Abstract ( 2027 )   Objective To observe pathological changes and apoptosis in mouse brain,hart,liver and kidney after tetramine poisoning,and to provide some references for forensic identification.Methods An experimental model of mouse tetramine poisoning was found,and the technology of apoptosis detection was used.The staining results were analyzed by computer image analysis competitive system.Results(1)Quantities of apoptosis in brain,hart,liver and kidney in chronic poisoning groups are many more than in acute poisoning groups.As in same organs,Quantities of apoptosis are different after different poisoning time.In addition,the peak-time of apoptosis is not same in different organs.(2)Poison analysis showed that,all organic samples from acute poisoning groups were positive,while that from chronic poisoning groups are negative.Conclusion Chronic tetramine poisoning can not be detective by clinic or poison analysis while can be found changes in apoptosis,which indicate that small amount and chronic poisoning still affects the body. Related Articles | Metrics

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Molecular cloning of recombinant fibronectin EDA and EDB fusion protein XUE AI-MIN;WANG HUA;YE RONG;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000) 2002, 0(3): 140-143.  Abstract ( 2186 )   Objective Construct a recombinant plasmid pET28a-EDA-EDB,prepare the fusion EDA-EDB protein.Methods For the production of recombinant fibronectin EDA-EDB in Escherichia coli,the EDA and EDB segments were separated from pGEM2-EDA/EDB and recomposed with two additional amino acids,then cloned into the expression vector pET28a.pET system to express EDA-EDB fusion protein and6 × His/Ni-NTA system to purify it in a single step were used.Western blotting confirmed the purified protein.Results The EDA and EDB segments were ligated and inserted into pET28a vector.EDA-EDB fusion protein was highly expressed in Escherichia coli BL21(DE3).Afterwards,it was purified by Ni-NTA resin and verified by western blotting.Conclusion EDA-EDB fusion protein can be expressed in pET system and purified by6 × His/Ni-NTA system. Related Articles | Metrics

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The relationship between postmortem degradation of marrow DNA in bosom bone and late postmortem interval estimation CHEN YU-CHUAN;CHENG JIAN-DING(DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY OF MEDI CAL SCIENCES;GUANGZHOU000) 2002, 0(3): 144-145.  Abstract ( 2025 )   Objective To study the relationship between degradation of marrow DNA and late postmortem interval(PMI).Methods Marrow were left on natural condition for0,1,3,5,7day after death respectively,Marrow DNA were detected by using Feulgen staining and computerized image analysis.Results The content of marrow DNA could be detected till7days afer death yet.Conclusion The degradation of marrow DNA may be used on estimation the late PMI. Related Articles | Metrics

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A primary study on the ARP-SRP gene expression profiling of brain injury by cDNA microarray ZHOU YI-WU;ZHANG YI-GU;DENG WEI-NIAN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COL LEGE OF HUST;WUHAN000) 2002, 0(3): 146-149+.  Abstract ( 1996 )   Objective To screen the differential expression of apoptosis-related protein and stress response protein(APR-SRP)genes after human brain injury by cDNA microrarray.Methods The total RNAs were isolated from normal and injured brain tissues of a car-accident victim,and were purified to obtain mRNAs by Oligotex.Both mRNAs from the tissues of the injured and the normal tissue were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes.The probes from normal tissue was labeled with Cy3-dUTP,that from the injured tissue with Cy5-dUTP.The mixed probes were hybridized to the BioDoor Chip ARP-SRP-1.0S,a cDNA microarray which contains77apoptosis related protein genes and23stress protein related genes.After high-stringent washing,the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues.Results Among the100target genes,Only CLN2gene(Homo sapiens lysosomal pepstatin insensitive protease gene)showed distinct deference in expression level between the brain injury and normal tissues.Conclusion cDNA microarray analysis indicated that CLN2gene,which is correlated to a fatal childhood neurodegenerative disease,might be related to the brain injury.The expression level of CLN2gene was significantly decreased in brain injured tissue in comparison to normal tissue.Further analysis of this gene will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine. Related Articles | Metrics

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Genetic polymorphism of the STR DXS9898 locus in Han population HUANG DAI-XIN ;LIANG WEI-BO ;WU MEI-YUN ;ET AL.(.FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000;.WEST CHINA FORENSIC MEDICINE COLLEGE;SICHUAN UNIVERSITY;CHENGDU0 2002, 0(3): 150-151+.  Abstract ( 1935 )   Objective Studying the genetic polymorphism of X-STR locus DXS9898in Han population.Methods296unrelated Chinese individuals(199females and97males)living in Chengdu were investigated using PCR and PAG electrophoresis followed by silver staining.Results6alleles were observed and the range of fragment size was189-214bp.The genotype distribution of DXS9898locus was in accordance with Hardy-Weinberg equilibrium.Family survey confirmed Mendelian inheritance of alleles.The observed heterozygosity in females was0.5930,the discriminating power(Dp)were0.5667and0.9420for males and females respectively.The power of exclusion were0.5862and0.4392for trio and duo respectively.Conclusion The results demonstrated that the locus is highly polymorphic and can be used in forensic identification and parentage testing. Related Articles | Metrics

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PGM_1 genotyping by PCR-SSCP SONG HAI-YAN;YANG QING-EN;YU CHUN-YING(DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000) 2002, 0(3): 152-154.  Abstract ( 2006 )   Objective PGM 1 genotyping by PCR-SSCP analysis.Methods Amplified genome DNA from156unrelated Han individuals living in Wuhan,PCR products for exon4and exon8of PGM 1 were then analyzed by SSCP to detect the genotypes.Results2alleles and3genotypes were detected in exon4and8respectively.The discrimination power was0.7318.PCR-SSCP analysis was suitable for determination of PGM 1 genotypes from old blood and semen stains.Conlusion PGM 1 system typed by PCR-SSCP is useful for forensic identification. Related Articles | Metrics

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Effect of PCR reaction volume on the accuracy of human identification tests ZHOU HUAI-GU;PING YUAN;XU QING-WEN;ET AL.(SHANGHAI INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY;SHANGHAI000) 2002, 0(3): 155-159.  Abstract ( 2136 )   Objective To investigate the effect of different PCR amplification volume on the accuracy of human identification test.Methods Human genome DNA samples were amplified using ABI PRISM Profiler Plus kits in50 μ l,25 μ l,12.5 μ l,and6.25 μ l reaction volume,respectively.The thermocycle parameters were the same.All PCR products were then electrophoresized on ABI PRISM310Genetic Analyzer,377DNA Sequencer,and3100Genetic Analyzer.Data were processed by ABI PRISM GeneScan and Genotyper software.Results The less re action volume,the more alleles losing or alleles adding observed.Conclusion Non-standard volume of PCR am plification reaction should be used carefully in human identification test,especially when the sample DNA quali ty is not so satisfied. Related Articles | Metrics

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Study on the forensic psychiatric examination of the cases of labor disputes GUAN WEI;HUANG FU-YIN;TANG TAO;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI000) 2002, 0(3): 160-163.  Abstract ( 1877 )   Objective To explore the current situation and tendency of the forensic psychiatric examination of the cases of labor disputes.Methods This study reviewed156cases examined during the period of1990-2001,analyzing some related factors.Results It was shown that most of these cases were related to the termination and interruption of labor contracts,while the quantity of cases related to labor pay in creased considerably.The intervals between the beginnings of disputes to examinations were prolonged,while69.2%of the intervals were within12months.91.2%conclusions of the examinations were consis tent with the diagnosis of psychiatric history.The rates of limited civil capability and incapability were23.1%and52.6%.Conclusion The quantity,type and interval of the cases of labor disputes were chang ing greatly during recent years.More attention should be paid to this kind of cases. Related Articles | Metrics

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Interpretation of DNA profiles for mixed stains LU DE-JIAN;LU HUI-LING;CHEN YU-CHUAN(DEPARTMENT OF FORENSIC SEROLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU;00) 2002, 0(3): 185-188.  Abstract ( 2117 )   This article review the advance in interpretation of mixed forensic stains using DNA profiling,including autosome STR profiling,sex profiling determined by PCR,Y-specific STR profiling,mitochondrial DNA profiling and single nucleotide polymorphism profiling.The statistics methods for mixed stain has also been reviewed. Related Articles | Metrics

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Medical legal identification of post-transfusion HIV/AIDS DAI ZHAO-YUN ;GU XIANG-YU ;WU JUN (.SHANGHAI HUADONG HOSPITAL;SHANGHAI0000;.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;PRC;SHANGHAI000) 2002, 0(3): 189-191.  Abstract ( 1987 )   This article summarized circumstances and influential factors of post-transfusion HIV/AIDS in recent years.Laws and regulations were emphasized in respective duties of every blood transfusion related departments.The strictly controlled imported blood products,carefully blood screening on donor,standardized blood products,tightened control on indication of use of blood,and finally,carefully told rare-happened HIV/AIDS to recipients were the key measures to avoid forensic cases of post-transfusion HIV/AIDS.Main evidences in Medical legal identification of post-transfusion HIV/AIDS were also proposed. Related Articles | Metrics