Abstract: Objective To investigate the effect of different PCR amplification volume on the accuracy of human identification test.Methods Human genome DNA samples were amplified using ABI PRISM Profiler Plus kits in50 μ l,25 μ l,12.5 μ l,and6.25 μ l reaction volume,respectively.The thermocycle parameters were the same.All PCR products were then electrophoresized on ABI PRISM310Genetic Analyzer,377DNA Sequencer,and3100Genetic Analyzer.Data were processed by ABI PRISM GeneScan and Genotyper software.Results The less re action volume,the more alleles losing or alleles adding observed.Conclusion Non-standard volume of PCR am plification reaction should be used carefully in human identification test,especially when the sample DNA quali ty is not so satisfied.

Key words: PCR amplification system, DNA analysis, allele loss, additional allele