PGM_1 genotyping by PCR-SSCP

›› 2002, Vol. 0 ›› Issue (3): 152-154.

PGM_1 genotyping by PCR-SSCP

SONG HAI-YAN,YANG QING-EN,YU CHUN-YING(DEPARTMENT OF FORENSIC MEDICINE,TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY,WUHAN430030)

Online:2002-06-25 Published:2002-06-28

Abstract

Abstract: Objective PGM 1 genotyping by PCR-SSCP analysis.Methods Amplified genome DNA from156unrelated Han individuals living in Wuhan,PCR products for exon4and exon8of PGM 1 were then analyzed by SSCP to detect the genotypes.Results2alleles and3genotypes were detected in exon4and8respectively.The discrimination power was0.7318.PCR-SSCP analysis was suitable for determination of PGM 1 genotypes from old blood and semen stains.Conlusion PGM 1 system typed by PCR-SSCP is useful for forensic identification.

Key words: PGM 1, genotype, PCR-SSCP

SONG HAI-YAN;YANG QING-EN;YU CHUN-YING(DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000). PGM_1 genotyping by PCR-SSCP[J]. , 2002, 0(3): 152-154.

PGM_1 genotyping by PCR-SSCP

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