Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats

Abstract

Abstract: Objective To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/ R) during acute period. Methods The model of I/R was established,and the rats was divided normal control,is-chmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes. Results After 10min ischemia and 30min reperfusion,the area of reperfusion showed a part of cardiac myocytes weak staining,and get to peak at I-60min/R-30min. The myocardium in normal control and ischemia groups less than 60min showed negative staining. No changes were found in all groups by HE staining. Conclusion c-fos mRNA detection may become an important method for diagnosis of I/R.

Key words: myocardial ischemia/reperfusion(I/R), c-fos mRNA, in situ hybridization

MA MENG-YUN;XU XIAOHU (SHENZHEN CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE;SHENZHEN 00;CHINA;COLLEGE OF MEDICAL SCIENCE;SHANTOU UNIVERSITY;SHANTOU 0;CHINA). Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats[J]. , 2003, 0(2): 65-67+1.

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Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats

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