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28 April 2003, Volume 19 Issue 2 Previous Issue    Next Issue
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Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats MA MENG-YUN;XU XIAOHU (SHENZHEN CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE;SHENZHEN 00;CHINA;COLLEGE OF MEDICAL SCIENCE;SHANTOU UNIVERSITY;SHANTOU 0;CHINA) 2003, 0(2): 65-67+1.  Abstract ( 1997 )   Objective To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/ R) during acute period. Methods The model of I/R was established,and the rats was divided normal control,is-chmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes. Results After 10min ischemia and 30min reperfusion,the area of reperfusion showed a part of cardiac myocytes weak staining,and get to peak at I-60min/R-30min. The myocardium in normal control and ischemia groups less than 60min showed negative staining. No changes were found in all groups by HE staining. Conclusion c-fos mRNA detection may become an important method for diagnosis of I/R. Related Articles | Metrics

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Study on the changes of ncNOS in chronic heroin dependence and spontaneous withdrawal in rats ZHANG GUO-HUA;WANG BAO-JIE;WU XU;ET AL. ( SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA) 2003, 0(2): 68-71.  Abstract ( 2047 )   Objective To study the changes and actions of neuronal constructive nitric oxide synthase(nc-NOS) in heroin drug abuse. Methods The expression of ncNOS and ncNOS mRNA in neurons of cerebral cortex,periaqueductal gray matter and the ventral tegmental area was observed by immunohistochemistry,in situ hybridization and image analysis technique after heroin dependence and spontaneous withdrawal in rats. Results The quantity of ncNOS and ncNOS mRNA rised clearly and the number of ncNOS and ncNOS mRNA positive cells increased greatly in heroin dependence and withdrawal. The changes of ncNOS and ncNOS mRNA in spontaneous withdrawal were more clear than ones of dependence. Heroin dependence and withdrawal led to alterations in ncNOS and ncNOS mRNA expression in important regions implicated in the physical tolerance and dependence. Conclusion The ncNOS plays an important role in heroin dependence and withdrawal. The ncNOS immunohistochemical changes observed in the present study might be useful for the forensic pathological diagnosis of heroin drug abuse. Related Articles | Metrics

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The development and changes of pressure of putrefactive gas in cadaveric enterocelia in spring and its forensic application LI ZONG-HUI;QIN GUANG-MING;ZHAO YI-CHENG;LI XIAO-LING;DONG XING-TING;MA WEN-XING ( SIMAO BRANCH;YUNNAN PROCURATORATE;SIMAO 000;CHINA;DEPARTMENT OF FORENSIC MEDICINE;KUNMING MEDICAL COLLEGE;KUNMING 00;CHINA;SIMAO PUBLIC HEALTH SCHOOL;SIMAO 000;C 2003, 0(2): 72-75.  Abstract ( 2138 )   Objective To study the development and changes of pressure of putrefactive gas (PPG) in cadaveric enterocelia in spring,and to explore its application in estimation of postmortem interval (EPI). Methods 57 goats were divided into 2 groups according to means of death,on land or in water. Celiac PPG were observed timely and systematically. Results The development of PPG in cadaveric enterocelia,which can be divided into raising phase,peak phase,and declining phase was observed,and a model to estimate postmortem interval by changes of PPG was founded. Conclusion Measuring PPG in cadaveric enterocelia could be used in forensic EPI. Related Articles | Metrics

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The relationship between the postmortem interval and the changes of DNA content in rabbit's cornea epithelial and endothelium by the computerized image analysis LIU CHENG-QUAN;XIONG PING (DEPARTMENT OF FORENSIC MEDICINE;NANHUA UNIVERSITY;HENGYANG 00;CHINA) 2003, 0(2): 76-77.  Abstract ( 2047 )   Objective To study the relationship between the postmortem interval (PMI)and the changes of DNA content in rabbit's cornea epithelial and endothelium. Methods Using the computerized image analysis to measure the DNA content in the cornea epithelial and endothelium of 105 rabbit's at every six hours during 72 hours after death . Results The degradation rate of DNA in nuclear has an apparent relationship in 72 hours after death of the rabbits. Conclusion The determination of the quantity of DNA in cornea nuclear is a new method to estimate the PMI accurately. Related Articles | Metrics

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A study on the relationship between the expression of apoptosis-related gene and the injury time in earlier period following cerebral contusion SONG XU-DONG;SHI WEI-DONG;QIN QI-SHENG (DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;DEPARTMENT OF TECHNOLOGY;PEOPLE'S COURT OF QINDAO;QINDAO 000;CHINA) 2003, 0(2): 78-80.  Abstract ( 2072 )   Objective To investigate the relationship between the expressive variety of apoptosis-related gene Bcl-2 ,Fas and the brain injury time during earlier period after cerebral contusion. Methods The morphologia feature of brain tissue was observed under microscopy and immunohistochemistry,image analyze technique was applied to detected the expression of Bcl-2 and Fas. Results Typical apoptotic cells were seen 8 hours after cerebral contusion. The expression of apoptosis-related gene was noted after 30 minutes and keep increase subsequently. The expression of Bcl-2 reached maximum level at 4 hours after brain trauma and then declined. Conclusion Detecting the expression of Bcl-2 and Fas would be of valuable in the distinguishment of promortem and postmortem injury,and the estimation of brain injury time during the earlier period after cerebral contusion. Related Articles | Metrics

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Experimental pathological study of subacute intoxication by Dioscorea bulbifera L SU LI;ZHU JIAN-HUA;CHENG LI-BAO;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;WANNAN MEDICAL COLLEGE;WUHU 00;CHINA) 2003, 0(2): 81-83.  Abstract ( 1921 )   Objective To study the pathological change and the toxic mechanism of Dioscorea bulbifera L in mice. Methods Sixty ICR mice were randomly assigned to four groups poisoned respectively with 200% Dioscorea bidbifera L of 1/4LD50,1/10LD50,1/30LD50 and a control group treated with distilled water by oral administration. All animals were pathologically examined with LM and some of them were examined with TEM when the mice died during the experiment or the survival mice were sacrificed after thirty days. Results The pathological changes showed fatty change and the increasing glycogen of liver cells;degeneration and necrosis of the epithelia of uriniferous tubules. The serum BUN and ALT of the experimental groups mice were higher than that of control group. Enzyme histochemical staining showed the decreasing activity of G-6-P and SDH in the liver cells in the experimental groups. Conclusion The experiment suggests that the target organs were liver and kidney. The toxic mechanism of Dioscorea bublifera L was the damage of the mitochondrional and endoplasmic reticulum membrane directly. As a result,the activity of the SDH and G-6-P decreased,the metabolism was affected. Related Articles | Metrics

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Age determinaton by radiographic feature of thyroid cartilage in female han population CHENG JIE;ZHAO XIAO-DONG;SU XIAO-QING;ET AL. (PINGXIANG PEOPLE'S PROCURATATORATE OF JIANGXI;PINGXI-ANG 000;CHINA;JIANGXI PEOPLE'S PROCURATATORATE;NANCHANG 0000;CHINA;PINGXIANG HOSPITAL;PINGXIANG 000;CHINA) 2003, 0(2): 84-85.  Abstract ( 2050 )   Objective To seek a method of age determination. Methods This sample consists of 69 female Chinese han individuals whose ages been known. Five positions were selected from their radiograms which have been divided into three or six grade and then set up a mathematical model to deduce age by multiple linear regression analysis. Results The standard deviation of female thyroid cartilage is 1.969 and the multiple correctation coefficient is 0.984. Concousion The observation proved that age determination by radio-graphic feature of thyroid cartilage can be used for female Chinese han population from 18 to 60 years old. Related Articles | Metrics

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A study on sarcosaphagous insects species variety with seasons in Chengdu WANG YE;LIU MIN;SUN DA-HONG;ET AL. (WEST CHINA BASIC MEDICINE & FORENSIC MEDICINE COLLEGE OF SICHUAN UNIVERSITY;CHENGDU 00;CHINA;BUREAU OF PUBLIC SECURITY OF CHENGDU;CHENGDU 0000;CHINA) 2003, 0(2): 86-87+9.  Abstract ( 2105 )   Objective To study the species of sarcosaphagous insects. Methods Rabbits were killed and placed outdoors from March to November. Flies that appeared the cadavers were observed and identified. Results There are five main flies,ie,Muscadomesticauicina,Lucilia sericata,C.Megacephala,S.fuscicauda,Aldrichiragrahormi. From midtime of April to the beginning of October,Muscadomesticauicina could be seen on the cadvers,however,Aldrichiragrahormi only could be seen before the beginning of May. Otherwise ,Lucilia sericata,C.Megacephala,S.fuscicauda could be seen on the cadvers from March to the end of October. After the midtime of November,none of sarcosaphagous flies could be seen on the cadvers. On the other hand,maggots of sarcosaphagous flies could be seen often on the cadvers after adults of sarcosaphagous flies intruding 1 to 4 days,relating to tempreture of environment. Conclusion If these flies history of life be studied,it is useful for estimating postmortem interval in Chengdu. Related Articles | Metrics

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Factors affecting the determination of the percent carboxyhemoglobin saturation of blood JIANG YAN;YE YANG-HONG;ZHANG YI-FAN;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000;CHINA;SHANGHAI PHARMACEUTICAL SCHOOL;SHANGHAI 00;CHINA) 2003, 0(2): 88-91.  Abstract ( 2318 )   Objective To investigate factors affecting the determination of the percent carboxyhemoglobin saturation (HbCO%) of blood in an attempt to offer further data for results interpretation and sample storage requirement. Methods The HbCO% of blood samples stored in various conditions were detected by three spec-trophotometries during the succeeding 30 days. Results The data detected by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry were more stable than mono-wavelength spec-trophotometry. The HbCO% of blood was significantly related with the storage conditions which include temperature,time and the degree of exposure to air. Conclusion Determinations of HbCO% are reliable which performed by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry,combine with spectral scans. During 30 days,blood stored at 4℃ exposed to a limited volume of air does not influence the determination of HbCO%. Related Articles | Metrics

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Estimation of postmortal interval by measuring the amount of DNA in cells CHEN XIN;SHEN YI-WEN;ZHAO ZI-QING (SHANGHAI CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE;SHANGHAI 000;CHINA;DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000;CHINA) 2003, 0(2): 120-121.  Abstract ( 2037 )   Although there are many methods to estimate the early postmortal interval,more attention has been paid to the research on measuring the amount of DNA in cells. This paper introduce several different measuring ways,law of variation and application situation of DNA in cells. In addition,the result evaluation of measuring methods and application prospect is given. Related Articles | Metrics

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The role of macrophages in wound age estimation during the wound healing process LI XUE-FENG;WANG HUI-JUN (DEPARTMENT OF PATHOLOGY;THE FIRST MILITARY MEDICAL UNIVERSITY;GUANGZHOU 0;CHINA) 2003, 0(2): 122-125.  Abstract ( 1923 )   The article reviewed the advance and the forensic meaning of macrophage on the wound age estimation during the wound healing process in resent years. It has also been summarized the relationship between macrophages and wound age estimation on the expression of cytokines derived from macrophages,the changes of macrophage phenotypes and the development process of phagosomes in macrophages after wounding. It is suggested that some regular and characteristic changes of macrophage should be a useful mark to wound age estimation and reminds therefore further study in this field. Related Articles | Metrics

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The research of the heroin and its metabolites analysis in clinical samples LIN LAN;LIAO LIN-CHUAN;YAN YOU-YI (WEST CHINA PRECLINICAL AND FORENSIC MEDICINE COLLEGE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA) 2003, 0(2): 126-128.  Abstract ( 2025 )   Heroin can be metabolized easily in body and the mail metabolites are 6-MAM,morphine and so on. At present,there are urine,blood,hair and so on as specimens for detection,while the analytical technology conclude TLC,GC,HPLC,GC/MS,LC/MS,IA,CE etc. In this paper,these technologies used for heroin's metabolites were viewed in order to provide some reference to the study in relative field. Related Articles | Metrics