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28 February 2003, Volume 19 Issue 1 Previous Issue    Next Issue
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GDNF expression following the severe brain injury in rats WANG XIN;LAO ZHI-GANG;LIU MIN;ET AL. (FORENSIC PATHOLOGY DEPARTMENT;SCHOOL OF LEGAL MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00) 2003, 0(1): 1-3.  Abstract ( 1546 )   Objective To evaluate the changes of GDNF after severe brain injury. Methods Changes GDNF in cortex, thalamus and pontine was observed by using immunohistochemistry and image analysis technique. Results The lever of GDNF was increasing at Id, reaching peak at 3d, not obviously decreasing at 5d and still higher than normal at 7d after severe brain injury. Conclusion As an objective indication, the time sequence regularity of GDNF after brain injury may be applied in brain injury time estimation. Related Articles | Metrics

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The study on expression of caspase-1 in experimental brain contusion of different severity in rats TAO LU-YANG;CHEN XI-PING;DING MEI;ET AL. (CHINA MEDICAL UNIVERSITY;SCHOOL OF FORENSIC MEDICINE;SHENYANG;P.R.CHINA 000) 2003, 0(1): 4-7+69.  Abstract ( 1998 )   Objective To investigate the expression of caspase-1 in different posttraumatic intervals and severity of brain injury. Methods The study examined brain tissue samples of slight (n=24), severe (n=24) brain injury and control (n=6) of rat, using immunohistochemical staining, western-blot and RT-PCR method. Results Up-regulating of caspase-1 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 2 weeks. Caspase-1 cleaved after brain injury. Conclusion These findings are the first time to demonstrate the study of different intense brain injury. The increasing of caspase-1 expression indicates that the TBI exists. The threshold area of caspase-1 positive cells is different in different severity of brain injury. Related Articles | Metrics

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A study on the expression of C-FOS protein after experimental rat brain concussion WANG FENG;LI YONG-HONG;HU YONG-LIANG (DEPARTMENT OF FORENSIC MEDICINE;WANNAN MEDICAL COLLEGE;WUHU 00) 2003, 0(1): 8-9.  Abstract ( 2027 )   Objective To study the relationship between expression of C-FOS protein and brain concussion and find a sensitive marker of diagnosis of the brain concussion. Methods Fifty-five rats were randomly divided into brain concussion groups and control group. The expression of C-FOS protein was microscopically observed by immunohistochemical method. Results There were negative expression of C-FOS protein in control group. In brain concussion group, however, positive expression of C-FOS protein in some neurons was seen at 15min after brain concussion, and reach to the peak at 6h after brain concussion, then decreased gradually. Conclusion These findings suggest that detection of C-FOS protein could be an index of diagnosis of brain concussion and a sensitive marker of timing of injury after brain concussion. Related Articles | Metrics

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A analysis of ELISA on the time-related expression of IL-2 and TNF-a during the healing process of wound in rat skin WANG HUI-JUN;DING YUN-CHUAN (DEPARTMENT OF PATHOLOGY;THE FIRST MILITARY MEDICAL UNIVERSITY;GUANGZHOU 0) 2003, 0(1): 10-12.  Abstract ( 2046 )   Objective In order to explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound. Methods A assay of ELISA were performed on intravital skin wounds(after incision 0.5-168 h) to detect the dynamics expression level of IL-2, TNF-a. Results The level of IL-2 and TNF-a increased at 0.5 h after wounding, then got to a peak at 3 h and 1 h after injure respectively. Rebound of TNF and IL-2 levels were shown at 48 h after wounding, and both cytokine levels were inclined to elevating between 72h and 168h after wounding. Conclusion The cytokine level changes suggest they have a time-related expression during wounds healing process. Related Articles | Metrics

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A study on the expression of VEGF in the blunt injury of rat's skin LI MET;HUANG PEI-JUN;LIU MIN;ET AL. (FORENSIC PATHOLOGY DEPARTMENT;SCHOOL OF LEGAL MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00) 2003, 0(1): 13-15+6.  Abstract ( 1906 )   Objective To evaluate changes of VEGF in skin after blunt injury. Methods The rats of injury groups were subjected to skin blunt injury by free-falling iron hammer. The samples taken at 1h, 3h, 6h, 12h,1d, 3d, 5d, 7d, 9d after injury were studied by immunohistochemistry and MIAS image analysis system. Results In the skin of normal control group the expression level of VEGF was low. The increase of VEGF could be observed at 1 day after injury, reached peak at 7 days and declined at 9 days. Conclusion Blunt injury in the skin could induce the expression of the VEGF. Moreover, the change pattern of VEGF level was quite regular with time and could be used to estimate the time after skin injury. Related Articles | Metrics

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Age determination by radiographic feature of thyroid cartilage in male Chinese han popullation CHENG JIE;ZHAO XIAO-DONG;SU XIAO-QING;ET AL. (PINGXIANG PEOPLE'S PROCURATATORATE OF JIANGXI;PINGXIANG 000;JIANGXI PEOPLE'S PROCURATATORATE;NANCHANG 0000;PINGXIANG HOSPITAL;PINGXIANG 000) 2003, 0(1): 16-17+2.  Abstract ( 1995 )   Objective To seek a exact method of age determination. Methods This sample consists of 73 male Chinese han individuals whose ages have been known. Eight positions were selected from their radiograms which have been divided into three or six grade and then set up a mathematical model to deduce age by multiple linear regression analysis. Results The standard deviation of male thyroid cartilage is 1.854 and the multiple correctation coefficient is 0.9971. Conclusion The observation proved that age determination by radiographic feature of thyroid cartiage may be suitable for male Chinese han population from 13 to 67 years old. Related Articles | Metrics

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Morphological changes of tissues after extensive soft tissue injury LI GE;LU JUN-BAO;YAO QING-SONG;ET AL. (PROCURATORATE OF YUEXIU DISTRICT;GUANGZHOU 00;DE-PARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY;GUANGZHOU 00;THE CRIMINAL SCIENCES RESEARCH INSTITUTE OF POLICE BUREAU OF GUANGZHOU;GUANGZHOU 2003, 0(1): 18-21.  Abstract ( 1998 )   Objective To study the pathological morphological changes for diagnosing the cause of death of extensive soft tissue injury or crush syndrome. Methods The tissues were stained by HE and IHC. Results (1)The Mb positive rate was 60%,75%,95% respectively.(2)Both the HSP70 positive rate of hearts and brains were 90%. Conclusion (1 )The animal model of broad soft tissue injury was established.(2) Accumulated the pathological morphological data for diagnosing the cause of death of extensive soft tissue injury or crush syndrome. Related Articles | Metrics

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The study of distortion products otoacoustic emissions in pseudo-anacousia WANG YAN (BEIJING INSTITUTE OF FORENSIC MEDICINE & SCIENCE;BEIJING 0000) 2003, 0(1): 22-23+2.  Abstract ( 1872 )   Objective To explore the technique that distinguish pseudo-anacousia. Methods 60 cases were divided into three groups ( normal group, deafness sensorineura group and pretending hearing loss group). They all had been tested by distortion products otoacoustic emissions technique . Results (1) The DPOAE incidence of normal group is 94.00%. the DPOAE incidence of deafness sensorineura group is 22.00%.There were significant differences at statistics in DPOAE incidence (P<0.01). those proved that patients (deafness sensorineura group ) were in hearing problem . (2) The DPOAE incidence of pretending hearing loss group is 93.00%,there were no significant differences at statistics in DPOAE incidence between normal group and pretending hearing loss group (P>0.01), furthermore when behavior threshold is over 50 dBHL , DPOAE incidence is no score . Conclusion This study shows the technique can distinguish pretending hearing loss . The application of DPOAE can be used as a new tool in cases assessment of clinic forensic medicine. Related Articles | Metrics

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Polymorphism of PentaD and PentaE STR locus in five Chinese Han population LIU QIU-LING;LU HUI-LING;LU DE-JIAN;ET AL. (FACULTY OF FORENSIC MEDICINE;ZHONGSHAN MEDICAL COLLEGE;ZHONGSHAN UNIVERSITY;GUANGZHOU 00) 2003, 0(1): 24-26.  Abstract ( 2192 )   Objective To obtain the genetic polymorphism data of Guangxi, Hunan, Henan, Sichuan ,Taiwang Chinese Han population and compare the polymorphism of PentaD and PentaE STR locus. Methods The two loci was analyzed by using the PowerPlex 16 System. Results 10 alleles of PentaD and 19 alleles of PentaE were found in the five Han population. PentaD and PentaE have the expected heterozygosity values of 0.774 6-0.804 7and 0.900 5-0.921 9,the polymorphism information content values of 0.771 0-0.802 5 and 0.896 9-0.917 6, the discrimination power values of 0.9223-0.934 1 and 0.947 1-0.978 2,the power of exclusion values of 0.5435-0.632 5 and 0.678 5-0.846 5, respectively. Conclusion The result showed that these two loci were highly informative and suitable for forensic application. Related Articles | Metrics

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New improved approaches for DYS385 detection TANG SHUANG-BO;GUO JING-YUAN;LI ZHAO-HUI;ET AL. (FACULITY OF FORENSIC MEDICINE;SUN YAT-SEN UNIVERSITY;GUANGZHOU 00) 2003, 0(1): 27-29.  Abstract ( 1879 )   Objective To establish more sensitive methods for detection of DYS385 in routine forensic casework. Methods The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition. Results It showed that an overall 112bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50pg. Conclusion This method is 20 fold more sensitive than the ordinary method. Related Articles | Metrics

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HLA gene polymorphism and forensic medicine JIN DA-QING;ZHENG XIU-FEN (CHINESE PEOPLE'S PUBLIC SECURITY UNIVERSITY;BEIJING 000) 2003, 0(1): 51-53.  Abstract ( 1892 )   The gene complex of Human Leukocyte Antigen (HLA) is located of chromosome 6p21, which is the most complicated dominant polymorphic genetic system. The HLA system has 108 genotypes. It is the best human genetic marker. It has been applied to forensic paternity test and individual identification. This article discusses the research development of HLA polymorphism and its application in forensic medicine. Related Articles | Metrics

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Trauma brain injury and apoptosis SHI WEI-DONG;WANG KONG-BAO;QIN QI-SHENG (PEOPLE'S COURT OF QINGDAO;QINGDAO 0;DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN;000) 2003, 0(1): 54-56.  Abstract ( 1923 )   The evidence and the feature of apoptosis following tyrauma brain injury(TBI) and the possible mechanisms underlying apoptosis were reviewed. Recently research showed that apoptosis play an important role in TBI, the occurring time and area of apoptosis were found significant differences compared with that of necrosis. The neural cell apoptosis can undergo following many pathways after TBI. In our review, the foreground of apoptosis after TBI research in forensic pathology were also discussed. Related Articles | Metrics

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Traumatic brain injury and caspase CHEN XI-PING;TAO LU-YANG;DING MEI;ET AL. (CHINA MEDICAL UNIVERSITY SCHOOL OF FORENSIC MEDICINE;SHENYANG 000) 2003, 0(1): 57-58.  Abstract ( 1855 )   Many pathologic and physiologic changes occur after brain injury. Many genes control these changes. Caspase plays an important role in neuron cell apoptosis and has concern with secondary brain injury. It is of great significance in the forensic science. Related Articles | Metrics

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The latest research progress on CGRP and its potential application in forensic medicine LI LEI-BO;HUANG PEI-JUN;LIAO ZHI-GANG;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;SI CHUAN UNIVERSITY;CHENGDU 00) 2003, 0(1): 59-61.  Abstract ( 1797 )   Calcitonin gene-related peptide (CGRP) play a key role in some physiological and pathological progresses. The latest studies indicate that CGRP might involve in some disease progress and has a close relation with wound healing. It is significant to further investigate and then apply it to clinical diagnosis and therapy as well as forensic pathology. Related Articles | Metrics

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The sudden cardiac death in negative autopsy YU XIAO-JUN;LI CHE;XU JIN-JIE (DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE OF SHANTOU UNIVERSITY;SHANTOU 0) 2003, 0(1): 62-66.  Abstract ( 1915 )   The advancement of studies about the molecular biology and electronic physiology on sudden cardiac death was summarized in this article, including particularly cardiac concussion (commotio cordis), congenital long QT syndrome, and Brugada syndrome which probably resulting in fatal arrhythmia and sudden cardiac death. These corpses of fatal functional disorders often show the results of negative autopsy without obvious organic pathological changes. So when come across negative autopsy the medical examiner and the pathologist should be careful to investigate the inductive cause of sudden death, the history of disease, and the family history, then to rule out the possibility of the above disorders. Related Articles | Metrics