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28 June 2003, Volume 19 Issue 3 Previous Issue    Next Issue
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Identification opportunity for several types of eye injuries XIA WEN-TAO ;DONG DA-AN ;YU HONG-WEI (.INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANG - HAI000;CHINA;.PEOPLE ' S PROCURATORATE OF SHANGHAI;SHANGHAI000;CHINA) 2003, 0(3): 129-132.  Abstract ( 1696 )   Objective To study the stability duration of visual acuity after eye injuries in several types,in order to define the earliest and best opportunity for identification.Methods160cases were divided into five groups,in which their stable visual acuity after injuries were divided into several ranges.Then the relationship between stability duration of visual acuity and their visual acuity level,as well as eye injury types were analyzed.Results Significant correlation existed between eye injury types,stable visual acuity level and stability duration of visual acuity after injuries.Conclusion The earliest and best opportunity for identification after eyes injuries related to injury types and visual acuity level. Related Articles | Metrics

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The compare disquisition of the collateral ligaments:normal and pathologic appearances on MRI ZHOU HUI;GE GANG;ZHOU WEI(THE MEDICOLEGAL EXPERTISE PLACE OF JIXI PUBLIC SECURITY;JIXI00;CHINA) 2003, 0(3): 133-135+.  Abstract ( 1811 )   Objective To evaluate the MR findings of normal and abnormal collateral ligament and the meaning of medical jurisprudence appraisal.Methods The MR findings of12normal and13surgically confirmed injured collateral ligaments were retrospectively reviewed and compared.Results The normal tibial and fibular collateral ligaments were hypointense string on both T 1 and T 2 coronal MR images,with average length of6.8cm for tibial collateral ligament and5.7cm for fibular collateral ligament.13collateral ligaments were injured,including6tibial and3fibular collateral ligaments.Two cases(4ligaments)had both tibial and fibularcollateral ligaments injuries.The injury of collateral ligament was classified as:Grade I(5ligaments),Grade II(5ligaments),and Grade III(3ligaments).The complications of anterior cruci - ate ligament sprains(3cases),posterior cruciate ligament sprains(4cases),meniscal tear(2cases)were found in7patients(63.6%).Conclusion Coronal MR imaging can clearly demonstrate the structure of the knee collateral ligaments,accurately diagnose and classify the ligament injury,and correctly detect the compli - cations,MRI is very valuable for medical jurisprudence appraisal. Related Articles | Metrics

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Expression of the glycoprotein in atrioventricular cell membrane of acute myocardial infarction in human LI ZHAO-HUI;JING HUA-LAN;LIU XIAO-SHAN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SUN YAT-SEN UNI-VERSITY;GUANGZHOU00;CHINA) 2003, 0(3): 136-137.  Abstract ( 1900 )   Objective To study the expression of the glycoprotein in atrioventricular cell membrane of the acute myocardial infarction.Methods The glycoprotein changes occurred at the atrioventricular cell membrane of the acute myocardial infarction of8cases were observed by using immunohistochemical methods.Results Positive staining of PNA could be observed in atrioventricular cell membrane.Conclu - sion This experiment proved that atrioventricular cell membrane expressed D-galactose as same as my - ocardial cell membrane in the acute myocardial infarction. Related Articles | Metrics

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The differences between left & right side development of osteophiphysis in estimating male juvenile age ZHENG JING;LU HUI-LING;CHEN LE(DEPARTMENT OF FORENSIC BIOLOGY;ZHONGSHAN MEDICAL COLLEGE;ZHONGSHAN UNIVERSITY;GUANGZHOU00;CHINA) 2003, 0(3): 138-139+.  Abstract ( 2006 )   Objective The influence on age estimation by comparing the differences between left and right side development of osteophiphysis.Methods27osteophiphysis development indexes in the X-ray films of6major joints and pelvises in130male juveniles were Cbserved.The positions and levels of difference between left and right side development of osteophiphysis were compared.The male juvenile age by re - gression equation according to left side and right side were estimated respectively.Results In76cases,the age estimation results are the same for both sides.In47cases,the difference between left and right side is less than6months.In7cases,the difference is between6and12months.The error of94.6%age estimation is within6months.The differences between left and right osteophiphysis development are often found in proximal humerus,distal radius,distal ulna and crista iliaca.Conclusion The subtle differ - ence between left and right osteophiphysis development has no influence on age estimation. Related Articles | Metrics

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The relationship between expression of FN in human skin injury and early injury time XUE AI-MIN;ZHAO ZI-QIN;SHEN YI-WEN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000 ;CHINA) 2003, 0(3): 140-142.  Abstract ( 1984 )   Objective In order to supply an effective reference of early injury time estimation and explore the time limit of detection of EDA\EDB mRNA in human skin samples,the expression of alternative splic-ing segment of fibronectin-EDA\EDB in incised wound of human skin were studied.Methods Using in situ hybridization with DIG-labeled anti-sense RNA probe,the expression of FN EDA\EDB domain was detected in human skin incised wound at the early stage of injury(from30min to3h).Results The posi-tive expression rates of FN-EDA\EDB immediately after injury and area far away from wound were same as the control group.The expression of FN-EDA\EDB in human skin incised wound showed a gradually increased tendency in early injury time(within3h).The positive expression cells were mainly distributed in basement cells of epidermis and the expression of EDA is much higher than EDB.It's difficult to detect EDA\EDB mRNA when the samples were deposited in air for4hour.Conclusion FN-EDA\EDB may be used as a sensitive mark for the estimation of early injury time.The in-situ hybridization technique is not applicable in the application. Related Articles | Metrics

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Genetic polymorphism and the population difference of 7 Y-STR loci and haplotypes in Chinese Han and J apanese populations ZHAO DONG;WANG BAO-JIE;DING MEI;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSI - TY;SHENYANG000;CHINA) 2003, 0(3): 143-148.  Abstract ( 1996 )   Objective To investigate the genetic polymorphism of7Y-STR loci and haplotypes and analyze their population difference.Methods The DNA samples from45Chinese Han and59Japanese men were ana-lyzed by PCR and the denatured PAGE followed by silver staining.Results33alleles were identified for6loci including DYS393,DYS389 Ⅰ ,DYS19,DYS390,DYS389 Ⅱ and DYS392,39alleles were identified for DYS385,the frequency distributions were between0.0169～0.6444,the DP value distributions of them were between0.5406～0.9579,the DP value of DYS385was the highest.With the comparison of data of the7loci,significant difference in genetics was observed between the two populations(P<0.05).There were95haplotypes that consist-ed of the7loci,Chinese Han had41haplotypes,DP value0.9960,Japanese population had54haplotypes,DP value0.9965,no common haplotypes were found between them.Conclusion The7STR loci above belong to loci of high discriminating ability,the haplotypes are highly polymorphic and their ethical character is apparent. Related Articles | Metrics

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The genetic polymorphism of 8 STR loci in northern Chinese Han and Uygur Populations WANG BAO-JIE;DING MEI;ZHAO DONG;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG000;CHINA) 2003, 0(3): 149-150+.  Abstract ( 2105 )   Objective The genetic polymorphism of8STR loci,was studied in northern Chinese Han and Uygur populations.Methods The8STR loci(vWA,TH01,TP0X,CSF1P0,D5S818,D13S317,D7S820,D16S539)were analyzed and genotyped by fluorescent prime labeling kit and gene scan technology.Results62alleles were found in100individuals of Han population,CPE value was0.9975;And52alleles were found in50individuals of uygur population,CPE value was0.9973.The TDP of both was beyond0.9999.The difference of gene frequency between the two populations was significant.Conclusion The genetic polymorphism of the8STR loci in the Han and uygur populations is high,there are ethic differences in frequency distribution. Related Articles | Metrics

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Genotype reliability of short tandem repeats typing from minute DNA LU DE-JIAN;SUN HONG-YU;CHEN LI-XIAN;ET AL.(DEPARTMENT OF FORENSIC BIOLOGY;ZHONGSHANG MEDICAL COLLEGE;GUANGZHOU00;CHINA) 2003, 0(3): 151-153.  Abstract ( 1999 )   Objective To understand the forensic practice of DNA profiling from minimal amount of DNA.Methods Serial dilutions of DNA were amplified with the PowerPlex TM 16System Kit,then the genotyping of short tandem repeat(STR)was performed by ABI377DNA automated Sequencer.Results When the mount of DNA template was less than250pg,allelic drop-out apparently occurred at several loci.Other disturbed peaks,such as artefact bands and imbalanced heterozygote,also presented.Conclusion The anomalous results may result in incorrect genotyping.Careful and comprehensive considerations are needed to interpret the STR profile of minute DNA. Related Articles | Metrics

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The research progression of fibronectin EDA's expression and functions XUE AI-MIN;ZHAO ZI-QIN(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000;CHINA) 2003, 0(3): 190-193.  Abstract ( 2055 )   Fibronectin is an adhesive extracellular matrix glycoprotein,it's primary gene transcription is alterna-tively spliced in three regions,designated EDA,EDB and IIICS.EDA segment potentiates the ability of FN to promote cell adhesion,migration,differentiation and proliferation,thus playing an important role in the process of wound healing,embryonic development and tumor metastasis.Here we discuss the alternatively splicing mecha-nism,biological properties and possible application of EDA. Related Articles | Metrics