Archive

28 August 2003, Volume 19 Issue 4 Previous Issue    Next Issue
---
论文


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

论文

Select

Distributions of haplotypes for three Y-STR loci in a Tibetan ethnic group of Chinese population by using Y-STR multiplexes YING BIN-WU;HOU YI-PING;TANG JIAN-PIN;ET AL.(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2003, 0(4): 193-195.  Abstract ( 2042 )   Objective One multiplex genotyping system was developed in using silver staining with allelic lad - ders for three Y-chromosome STR markers(DYS434,DYS443,and DYS456),with a view towards the applica - tion of rapid and simple genotyping assay methods for DNA profiling.The distributions of haplotypes for three Y-STR loci(DYS434,DYS443,and DYS456)was investigated in a Tibetan ethnic group of Chinese population.Methods Allele and haplotype frequencies at these Y-STRs loci(DYS434,DYS443,and DYS456)were analysed by PCR amplification using Y-STR multiplexes,followed by horizontal non-denaturing polyacrylamide gelelec-trophoresis in101unrelated males of Tibetan ethnic group in Lasa of China.Results A total of31different haplotypes were found,16of them being unique.The haplotype diversity value(which is the same as the dis - crimination index)calculated from all three loci combined was0.9481,which is informative.Conclution The Y-STR multiplexes provide useful information for forensic analysis and paternity tests and can also be of great ben - efit for providing information not normally available from autosomal DNA systems. Related Articles | Metrics

Select

Seven Y-specific loci haplotypes and their usefulness in forensic casework LIU QIU-LING;LU DE-JIAN;CHEN LI-XIAN;ET AL.(FAULTY OF FORENSIC MEDICINE;ZHONG SHAN MEDICAL COLLEGE;ZHONG SHAN UNIVERSITY;GUANGZHOU00;CHINA) 2003, 0(4): 196-198+.  Abstract ( 1913 )   Objective To study genetic polymorphism of7Y-specific short tandem repeats(STR)and as-sess their usefulness in forensic casework.Methods7Y-STR have been amplified in two multiplex reac-tions,(Multiplex I:DYS391,GATA-A4,GATA-A10and GATA-H4.Multiplex II:DYS439,DYS437and DYS434).PCR products were separated by polyacrylamide gels electrophoresis followed by silver stain.Results When372unrelated individuals from the Han population in Guangdong were detected by those system,DYS391,GATA-A4,GATA-A10,GATA-H4,DYS439,DYS437and DYS434showed5,7,6,5,6,4,4al-leles,respectively.A total of254different haplotypes were identified,of which201(79.13%)were found in single individuals.The overall haplotypes diversity reached0.9960.Conclusion The7Y-STR loci are highly genetic polymorphism and they will be very powerful for establishing Y-STR database,understand-ing human origin,paternity testing and personal identification. Related Articles | Metrics

Select

DNA analysis of a 500 year mummy sample TONG DA-YUE;WU XIN-YAO;CAI GUI-QING;ET AL.(FACULTY OF FORENSIC MEDICINE;ZHONGSHAN MEDICAL COL-LEGE;ZHONGSHAN UNIVERSITY;GUANGZHOU00;CHINA) 2003, 0(4): 199-200.  Abstract ( 1862 )   Objective To accumulate experience for dated forensic matter anaylsis,for example,Mummy.Methods DNA are extracted by methods of phenol-chloroform and are purified by Wizard DNA clean-up system.The STRs locus are ampolification with Promega Powerplus16system.The mtDNA hypervariable region1(HV1)is amplificated by'3pair primers'.The products were sequenced with377DNA sequencer.Results The STRs locus very distinctness and mtDNA sequence is correct.Conclusion It is a valuable method for special forensic matters. Related Articles | Metrics

Select

Study on the dynamic changing profiles of telomeric restricted fragment length among sex balanced different age groups GE LU-LU ;LIU CHAO ;TAO LI-YANG ;ET AL.(.GUANGZHOU INSTITUTE OF CRIMINAL SCIENCE&TECHNOLOGY;GUANGZHOU000;CHINA;.DEPARTMENT OF FORENSIC BIOLOGY;ZHONGSHAN MEDICAL COLLEGE;ZHONGSHAN U - NIVERSITY;GUANGZHOU00;CHINA) 2003, 0(4): 201-203.  Abstract ( 1906 )   Objective In human,both in vivo and in vitro,telomere shortening appears to be a major component of cell senescence and aging.The detailed telomere shortening status and mechanism in pe - ripheral blood cell is needed to be further characterized.Methods One hundred and twenty three periph - eral blood samples were collected from healthy individuals of different age groups and the mean telomeric restricted fragment(TRF)was measured using Southern Blotting with Dig labeled probe.The samples of different groups were homogenized in sex components as indicated byχ 2 test of sex ratio of different test groups(P>0.05).Results The average length of TRF is shortening with aging and distinguished shortening dynamic profiles could be observed.Further analysis showed that there might be a shortening peak near the age of5.Conclusion There are distinguished dynamics profiles of telomere shortening among different age groups.Thus,the results indicate that it might be possible to infer individual age by telomeric re - stricted fragment length assay. Related Articles | Metrics

Select

Genetic studies of 15 STR loci in Guangxi Miao population LIU CHAO;YANG DIAN;LIU CHANG-HUI;ET AL.(GUANGZHOU CRIMINAL SCIENCE&TECHNOLOGY INSTITUTE;GUANGZHOU000;CHINA)[ 2003, 0(4): 204-206.  Abstract ( 1996 )   Objective The study was carried out to investigate genetic polymorphism of15STR loci in Guangxi Miao population.Methords DNA samples from southern China274Miao unrelated individuals were screened by using AmpFlSTR Identifiler TM PCR Amplification Kit and3100Genetic Analyzer.Results These15loci meet the Hardy-Weinberg expectations.The matching probability of the15STR loci was5.04 × 10 -17 .and combined pa - ternity of exclution was0.9999993in Guangxi Miao population.Conclusion Our results showed Identifiler TM PCR Amplification systems of15STR loci were useful enough to forensic case work in Guangxi Miao population. Related Articles | Metrics

Select

The expression of Bcl-2 protein on acute myocardial ischemia/reperfusion and the significance in forensic pathology WANG FENG;HU YONG-LIANG;LI YONG-HONG(DEPARTMENT OF FORENSIC PATHOLOGY;WANNAN MEDICAL COLLEGE;WUHU00;CHINA) 2003, 0(4): 207-208+.  Abstract ( 1920 )   Objective To search the objective morphologic evidence for the forensic pathological diagnosis on the acute myocardial ischemia/reperfusion.Methods The expression of Bcl-2protein in the early stage of acute myocardial ischemia/reperfusion(I/R)in rats were studyed with immunohistochemistry.Results There was no expression of Bcl-2protein in control group.The expression of Bcl-2protein began to increase in myocytes0.5h after I/R in the ischemic areas and the expression of Bcl-2protein in inner layer was stronger than in outer layer.The expression of Bcl-2protein appears firstly in the inner layer of my - ocardium and extended gradually toward the outer layer1h after I/R.It was found that the expression of Bcl-2protein in outer layer was stronger than in inner layer,then the expression of Bcl-2protein be - came weaken gradually.Conclusion Bcl-2gene may be an important regulative gene in the process of apoptosis and the changes observed in this paper might be useful for the forensic pathological diagnosis on the early myocardial ischemia. Related Articles | Metrics

Select

Identification of limited capacity for duties in schizophrenia:review of 31cases TANG TAO;CAI WEI-XIONG;WU JIA-SHENG(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI000;CHINA) 2003, 0(4): 209-211.  Abstract ( 1999 )   Objective To study general rule existed in identification of limited capacity for duties in schizophre-nia and put forward some recommendations to the related issues.Methods The data of31cases were analyzed based on essential items in identification of limited capacity for duties in Schizophrenia in order to obtain the contribution rate of major variables.Results It showed that the contribution rate of from variable1to variable6was18.785%,15.549%,14.023%,10.347%,9.437%and7.923%respectively,in which the variable1reflecting patient's recognition of dangerous act was the most important of all variables.Conclusion The limited capacity for duties in Schizophrenia could be divided into three grades. Related Articles | Metrics

Select

Determination of the Hemoglobin in Poisoned Blood by Spectrophotometery CHU JIAN-XIN ;MAN QIN ;BAO CHAO-SHENG ;ET AL.(.PROCURATORIAL SCIENCE INSTITUTE OF PEOPLE'S PROCU-RATORATE OF ZHEJIANG PROVINCE;HANGZHOU00;CHINA;.PUBLIC SECURITY TRAINING SCHOOL OF ZHEJIANG PROVINCE;HANGZHOU00;CHINA) 2003, 0(4): 212-214.  Abstract ( 2086 )   Objective In order to establish a objective method of analysis in the case of sulfured hydrogen poison.Methods The sulfured hemoglobin of the biomaterials(blood)were investigated by the spectropho - tometery.Results Results showed that sulfured hemoglobin had a specific absorbance peak at612nm,it is a linear relationship about the absorbancity to the concentration of sulfured hemoglobin.Conclusion It is possible to mark the poison degree by the spectrophotometery. Related Articles | Metrics

Select

Advances of study on the formulae based on a correlation and linear regression between the potassium concentration in the vitreous humor and the post-mortem interval and the factors which modifies the re-lationship CAI JI-FENG;TAO TAO;PAN HONG-FU;ET AL.(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNI-VERSITY;CHENGDU00;CHINA)) 2003, 0(4): 239-241.  Abstract ( 1982 )   In this paper we reviewed the formulae and analyzed the factors which modified the relationship and esti-mate the time of death as precise as possible by potassium concentration in vitreous humor.The extra factors including numbers of the cases,cause of death,different eye of both,the urea or/and creatinine in vitreous humor,environmental temperature,sampling techniques,experimental and analytical method were also mentioned. Related Articles | Metrics

Select

Cardiac troponin T and I:application in myocardial injury and forensic medicine XING YU;HUANG PEI-JUN;ZHANG KUI-MING;ET AL.(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2003, 0(4): 242-244.  Abstract ( 2048 )   The Cardiac Troponin T and I are highly cardiac specific biochemical markers of myocardial injury.They are very sensitive markers to detect all kinds of myocardial injury,and are able to distinguish myocardial injury and skeletal injury.Furthermore,They are independent predictor of future cardiac events.Such markers are now widely used in the clinic practice.It is prospective to use them in Forensic Medical Science. Related Articles | Metrics

Select

DNA-based technique and species identification ZHOU BIN;ZHANG LIN;WU MEI-YUN;ET AL.(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN U-NIVERSITY;CHENGDU00;CHINA) 2003, 0(4): 245-248+.  Abstract ( 2132 )   The species identification plays a key role in forensic analysis.Generally,three methods have been ap-plied for this purpose,they are morphologic-based,serologic-based and DNA-based techniques.This review mainly discussed the DNA-based technique and evaluate it's value in species identification of forensic science. Related Articles | Metrics

Select

Proceeding of research on X chromosome genetic markers ZHANG WEI-JUAN;ZHOU XUE-PING;JIA ZHEN-JUN;ET AL(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2003, 0(4): 249-252.  Abstract ( 1954 )   The development of Human Genome Project(HGP)makes it possible and more important to reveal the variations or polymorphisms precisely between different individuals and populations.Due to the characters of their high polymorphism and value in disease-linkage analysis as well as pharmacogenomnics,genetic markers on X chromosome have attracted much more attention of current medical and forensic scientists.This report summarized the proceeding of research on X chromosome genetic markers in the clinical and forensic context. Related Articles | Metrics

Select

SUBJECT INDEX IN ENGLISH OF VOLUME 19(2003)(MAIN ARTICLES) 2003, 0(4): 265-265.  Abstract ( 1114 )   Related Articles | Metrics