Abstract: Objective To search the objective morphologic evidence for the forensic pathological diagnosis on the acute myocardial ischemia/reperfusion.Methods The expression of Bcl-2protein in the early stage of acute myocardial ischemia/reperfusion(I/R)in rats were studyed with immunohistochemistry.Results There was no expression of Bcl-2protein in control group.The expression of Bcl-2protein began to increase in myocytes0.5h after I/R in the ischemic areas and the expression of Bcl-2protein in inner layer was stronger than in outer layer.The expression of Bcl-2protein appears firstly in the inner layer of my - ocardium and extended gradually toward the outer layer1h after I/R.It was found that the expression of Bcl-2protein in outer layer was stronger than in inner layer,then the expression of Bcl-2protein be - came weaken gradually.Conclusion Bcl-2gene may be an important regulative gene in the process of apoptosis and the changes observed in this paper might be useful for the forensic pathological diagnosis on the early myocardial ischemia.

Key words: myocardical cell, ischemia/reperfusion, Bcl-2 protein, immunohistochemistry, forensic pathology