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28 February 2004, Volume 20 Issue 1 Previous Issue    Next Issue
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An immunohistochemical study on the expression of caspase-3 in the skin wound healing DU YU;GUAN DA-WEI;ZHAO RUI(SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL U NIVERSITY;SHENYANG000;CHINA) 2004, 0(1): 1-3+69.  Abstract ( 2013 )   Objective To investigate the expressi on of caspase-3during skin wound healing and explore the applicability of caspase-3to determination of wound age.Methods The expression of caspase-3were performed on33mice skin incised wounds at different posttraumatic intervals and3mice as c ontrol by immunohistochemical technique.Results Expression of caspase-3was detec table in polymorphonuclear cells(PMNs)in the wound specimens aged6h.In the wound specimens aged from12h to24h after injury,caspase-3was identified in a large nu mber of infiltrating PMNs and part of mononuclear cells(MNCs).Afterwards,the MNC s and fibroblastic cells(FBCs)accounted in the most part of the caspase-3positiv e cells.Morphometrically,the ratio of the number of the caspase-3stained PMNs,MN Cs and FBCs to total number of the cells in the wounds was evaluated and calcula ted.The ratio of the caspase-3positive cells was low in the wound specimens aged from0h to3h(4.53 ± 6.53)%,and maximized in the wound speci-mens aged3day(62.66 ± 4.84)%.Thereafter,the ratio decreased and minimized in the specimens aged14d ay(21.56 ± 4.54)%.Conclusion The results suggest that caspase-3may play an important ro le in inducing neutrophil,macrophage and fibroblast apoptosis during skin wound healing.Furthermore,caspase-3may be used as a marker for the wound age determination. Related Articles | Metrics

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The dynamically changes of COX-2and the gene expression of COX-2mRNA after traumatic brain injury in rats WU XU;WANG BAO-JIE;ZHANG GUO-HUA;ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY;SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG000;CHINA) 2004, 0(1): 4-6+8.  Abstract ( 1975 )   Objective To illuminate the pathology of traumatic brain injury(TBI)and to better understand the re-lationship between the expression of COX-2and the time course of TBI.Methods Immunocytochemical staining,double-labeled with the anti-COX-2antibody and either the neuron-specific antibody NeuN or the astroglial-spe cific marker GFAP,in situ hybridization and computer image analysis were used.Re sults Results from im-munohistochemistry indicated time-dependent staining chan ges of neuronal plasma.The immunostained cells were faint at control cortex,most ly were neurons.The immunostained cells appeared to be darkly stained30min after TBI for extended periods of time and reached the maximum at2d after injury,reac hed another peak(P<0.05)at4d post-injury.The darker cells persisted in a high le vel,significant differences(P<0.05)even presented between control and15d post-in jury.The COX-2mRNA expression was faint at control cortex.The expressions of COX-2mRNA appeared to be darkly stained15min after TBI for extended periods of time and reached the maximum(P<0.05)at1d post-injury,reached another peak(P<0.05)at3 d post-in-jury,and significant differences(P<0.05)even presented between contro l and7d post-injury,but not15d post-injury.Conclusion The results of this study indicated that the expression of COX-2mRNA and protein had a possible relationsh ip with the extended periods of time after TBI.It might have some relationship b e-tween the expression of COX-2and secondary brain injury after TBI. Related Articles | Metrics

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Correlation study between the connexin43 and the myocardial ischemia in rabbit CHEN YANG;HU HUAZI;LIAO ZHIGANG;ET AL.(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDIC INE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2004, 0(1): 7-8+70.  Abstract ( 2035 )   Objective To study the co rr elation between quantity of the connexin43express and ischemia time in rabbit.Me thods Left coronary arteries were ligated to create early myocardial ischemia du ring dis-tinct time,HBFP staining was used in the ischemia spot and SP method was use d to show the expression of connexin43and the ischemia area were measured by the computer image analysis.Results Negative correlation was observed between the c onnexin43quantity and the ischemia time.The significant was dif-ference.Conclusion With ischemia time raised,expression of connexin43was des cented. Related Articles | Metrics

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The study on the expression of caspase-3 in experimental brain contusion in rats TAO LU-YANG;CHEN XI-PING;BIAN SHI-ZHONG;ET AL.(SCHOOL OF MEDICINE;SUZHOU UNIVERSITY;SUZHOU00;CHINA) 2004, 0(1): 9-12+7.  Abstract ( 2149 )   Objective To investigate the expression of caspase-3in different posttraumati c intervals and severity of brain injury.Methods The study examined brain tissue samples of slight(n=24),severe(n=24)brain injury and control(n=6)of rat,using i mmunohistochemical staining,western-blot and RT-PCR method.Results Up-regulating of caspase-3expression was found in tissue from traumatic brain injury compared with controls in early1hour after TBI,and lasted for14days.The gray degree and threshold area of caspase-3positive cells is different in different severity of brain injury.Conclusion The increasing of caspase-3expression indicates that TBI exists.The gray degree,threshold area of caspase-3positive cells and the cleava ge degree of pro-caspase-3have association with the severity of brain injury. Related Articles | Metrics

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Expression of water channel protein1in the lung of the drown rat HU HUA-ZI;CHEN YANG;LIAO ZHI-GANG;ET AL.(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2004, 0(1): 13-14+7.  Abstract ( 2528 )   Objective To determine the expression of water channel1protein in the lung of drown rat and that of after death thrown into the water.Methods Immunohistochemical method was used and the computer image analysis was conducted to detect the distribution of AQP1.Results The positive expres-sion of AQP1was seen in the capillary endotheliocyte of the interstitial and around bronchi and in the alveolar endothelial cells.The value of intergrated optical density was statistical significant.Conclusion It suggests that AQP1is one of sensitive signs to distinguish ante-mortem and postmortem immersion. Related Articles | Metrics

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Immunohistochemical observation of heart-type fatty acid binding-protein in early myocardial ischemia MING MEI;MENG XIANG-ZHI(DEPARTMENT OF FORENSIC MEDICINE;SCHOOL OF MEDICINE;WUHAN UNIVERSITY;WUHAN00;CHINA) 2004, 0(1): 15-17+7.  Abstract ( 2065 )   Objective The sensitivity of heart-type fatty acid binding-protein(H-FABP)in the postmortem diagnosis of myocardial ischemia was explored.Methods The changes of H-FABP staining in normal,in - farcted and suspected ischemia of myocardial cells were studied by immunohistochemistry.Results There was no depletion in normal control group,and obvious depletion was observed in myocardial infarcted group.Among9suspected myocardial ischemia group,3cases showed obvious depletion and3cases showed vague depletion for H-FABP,there were obvious depletion of Mb in4suspected myocardial is - chemia cases and vague depletion in2cases for Mb.It is indicated that H-FABP can be used to diag - nose early myocardial ischemia.Conclusion H-FABP is quite sensitive and useful for the diagnosis of early myocardial ischemia. Related Articles | Metrics

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Inferior orbital fissure and groove:axial CT findings and their anatomic variation XU LEI-MING;ZHANG SHI-ZHENG;XIE XING-FU(DEPARTMENT OF RADIOLOGY;ND HOSPITAL ATTACHED TO ZHE - JIANG UNIVERSITY MEDICAL SCHOOL;HANGZHOU000;CHINA) 2004, 0(1): 18-20.  Abstract ( 2149 )   Objective To show imaging findings of inferior orbital fissure(IOF)and groove(IOG)on axial CT scans and to discover their anatomic variations,so as to avoid misdiagnosing them as orbital fracture.Methods25normal skull were used to investigate the configurations of IOF and IOG.Five skulls were performed axial CT scans.20normal orbital axial scans were studied as well.MPR and RT-3D recon-structions were used in this study.Results skulls scans and normal orbital images on axial CT showed three sorts of findings:(1)single bony dehiscence between lateral and inferior walls;(2)first type of dou-ble bony dehiscence between lateral and inferior walls,among the dehiscence interposing a small bone.The long axis of the small bone was parallel to orbital wall;(3)second type of double bony dehiscence between lateral and inferior walls,but the long axis of the small bone was in anteroposterior direction.Anatomy and variation of three sorts of CT findings were corresponded respectively to:(1)a baseball club-shaped IOF;(2)a"V"-shaped IOF,that is composed of both of lateral and internal ramus,lateral ramus situates between the zygoma and the lateral portion of greater wing of sphenoid,and internal ramus between the maxilla and the internal portion of greater wing of sphenoid,both rami intercross caudally and open upwards in a"V"-shaped configuration;(3)a deep IOG with a protuberant lateral wall.Con - clusion Familiarity of imaging features on the axial CT scans and understanding of their anatomy of IOF and IOG would be helpful for avoiding misdiagnosis of orbital fracture. Related Articles | Metrics

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A population study of three Y-STR loci by multiple xing in Han population in Chengdu,China JIA YI ;ZHANG LIN ;WU MEI-YUN (.WEST CHINA PRECLINICAL AND FORENSIC MEDICINE COLLEGE;SICHUAN UNIVERSITY;C HENGDU00;CHINA;.KEY LABORATORY OF BIOTHERAPY OF HUMAN DISEASES;MINISTRY OF EDU-CATION;CHENGDU00;CHINA)[ 2004, 0(1): 21-22.  Abstract ( 1621 )   Objective To establish a better met ho d determing three Y-STR loci.Methods A multiplex of Y-STRs and amplify simultane ously three Y-STRs loci.Results Establish successfully a multiplexing sys - tem of three Y-STR loci(DYS390,DYS391and DYS393)followed by a population gen etic study of Han population in Chengdu,China.Conculusion The diversity of haplo type is0.8965,the value of discrimina - tion and the chance of exclusion chance is0.8965with the standard error0.008 1.This established system is one of the good tools in personnal identification a nd genetic study.[ Related Articles | Metrics

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A study on the effect of methanal during determination of carbon monoxide in blood YAN YOU-YI;LIAO LIN-CHUAN;YANG LIN;ET AL.(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC&MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2004, 0(1): 23-25.  Abstract ( 1986 )   Objective To confirm whether formaldehyde disturb detecting carbon monoxide in blood.To give an evidence that can be used for detecting carboxyhemoglobin more accurately in carbon monoxide poisoning ap-praises.Methods Blood samples came from carbon monoxide poisoning and the health were collected.Regular methods for detecting carboxyhemoglobin were used.Observing and comparing the detection results between which were spiked with methanal and no spiked one were performed.Results Methanal will affect the result of following experiments such as heating,adding NaOH,absorbed by PdCl 2 and spectrophotometry.Conclusion The samples which contaminated by formaldehyde couldn't be used for detecting carboxyhemoglobin. Related Articles | Metrics

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Arrhythmogenic right ventricular cardiomyopathy WANG QI-WEI;LIU LIANG(FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000;CHINA) 2004, 0(1): 35-36+3.  Abstract ( 1984 )   Arrhythmogenic right ventricular cardiomyopathy(ARVC)is a heart muscle disease of unknown etiology characterized by fatty or fibrofatty infiltration of the right ventricular myocardium.It is well known that ARVC is one of the important causes of sudden death in young individuals.The significant pathological change of ARVC is atrophy and fibrofatty myocardial replacement of the right ventricular free wall.According to the histopathological substrate ARVC could be divided into2types:fatty and fibrofatty myocardial replacement.Possible etiology in-cluding:(1)apoptosis or programmed cell death;(2)inflammatory;(3)myocardium dysplasia and(4)myocyte trans-differentiation theory. Related Articles | Metrics

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The study of status and advances on tetramine poisoning ZHU CHUAN-HONG ;LIU LIANG ;LIU YAN ;ET AL.(.FORENSIC SCIENCE INSTITUTE OF WUHAN PUBLIC SECURITY BUREAU;WUHAN00;CHINA;.FACUCTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000;CHINA) 2004, 0(1): 37-39.  Abstract ( 2131 )   Tetramethylenedisulphotetramine(TETS)is virulent rodenticides which was strictly forbidden to use in China.Poison dose of TETS is very little,LD 50 in rats is0.1mg/kg.Manifestations and signs of TETS poisoning are showed in5min.The long dated effect of poisoning is extremely strict.Mamy studys on TETS are restricted on the treatment of TETS poisoning,while that of poisoning mechanism is vevy few.This paper reviewed TETS poisoning mechanism,patho-logical changes and research advances. Related Articles | Metrics

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MVR-PCR and forensic medicine WANG JI-QIU;DING MEI;SUN ZHE;ET AL.(FORENSIC COLLEGE OF CHINA MEDICAL UNIVERSITY;SHENGYANG000;CHINA) 2004, 0(1): 40-43.  Abstract ( 2112 )   MVR mapping is one kind of DNA analytic techniques,which has developed greatly within13years.The molecular basis,history and applications in forensic medicine were systemically reviewed in this paper.Be-cause of its unsubstitutive mole in forensic paternity test and individual identification,the technique is becoming one of the important methods of bioassay. Related Articles | Metrics