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28 April 2004, Volume 20 Issue 2 Previous Issue    Next Issue
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The expression of bFGF and its receptor FGFR1 in rat after fluid-percussive brain injury HUANG DAI-XIN;ZHANG LIN;WU MEI-YUN;ET AL. (.FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;. COLLEGE OF ELEMENTARY MEDICINE AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENG 2004, 0(2): 65-67+1.  Abstract ( 2071 )   Objective Study on the pattern of changes of bFGF and FGFR1 immunoreactivity occurred in the experimental brain injury model for the purpose of providing the scientific basis for molecular pathological diagnosis, forensic identification, clinical treatment as well as further ascertaining the molecular mechanism of brain injury. Methods Male SD rats were divided into normal control, sham operation control and injury groups. The rats of injury groups were subjected to moderate lateral fluid percussion brain injury (0.2 mPa). The injury groups were then subdivided into 30 min, 1,3,6,12 h,1,3,7 d groups according to the time elapsed after injury. The SP immunohistochemistry method was used to examine the expression of both bFGF and FGFR1 factors in rat brain. Results In the brain of normal control and sham operation control groups, the low expression levels of bFGF and FGFR1 were observed. The increase of bFGF and FGFR1 immunoreactivity could be observed 6 h after injury in cortex and brain stem, reached to the peak at 1 d and remained at the high level up to 3 d, then partly declined at 7 d. In hippocampus, however, the increase occur as early as 3 h after injury, reached to the peak at 1 d and then decreased progressively, and returned to basal level at 7 d. Conclusion The results suggested that brain injury induced the gene expressions of bFGF and FGFR1. The bFGF may contribute to maintenance of nerve cell survival and the repair of damaged neural tissues after CNS injury and the patterns of their level change were quite regular and can be used for timing of injury in forensic medicine aspect. Related Articles | Metrics

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Investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells of rat LIU ZHI-PING;CHEN XIN;SHEN YI-WEN;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000;CHINA) 2004, 0(2): 68-69.  Abstract ( 2197 )   Objective To investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells. Methods After different PMI from the heart, liver, spleen and kidney were taken into pieces respectively, then centrifuged and digested to get suspending cells fluid. The amount of DNA of rats′viscera were detected by flow cytometry after stained by fluorescence , and also inspect the amount of DNA in different periods to find out the law of its variation. Results It showed a descendent trend of the amount of DNA in cells after different PMI, especially in spleen. Conclusion The amount of DNA of all the viscera grows downwards after death, this might be applyed in forensic estimation of PMI. Related Articles | Metrics

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Immunohistochemical and morphometrical study on the expression of interleukin-10 (IL-10) in different expressive parts during cutaneous wound healing in mice ZHANG HUI;ZHU SHAO-HUA;QIN QI-SHENG;ET AL. ( DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA ) 2004, 0(2): 70-72+1.  Abstract ( 2037 )   Objective To explore the expression of interleukin-10 (IL-10) in different expressive parts and its relationship with the wound age during cutaneous wound healing in mice. Methods Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. Results In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells. The expression of IL-10 in epidermal cells was already enhanced since 1～3 h after incision, and it returned to lower lever by 24 h, but increased again at 48 h after injury. IL-10 immunoreactivive cells under epidermis were mainly infiltrating mononuclear cells. Morphometrically, the ratio of the number of infiltrating cells was evaluated. A subsequent increase in the IL-10 positive cells was observed in the granulation tissue beyond the wound and in dermal at 6 h after incision, and the maximum ratio was (51.41±3.12)% in a 72 h-old wound. Then the positive cells gradually decreased (29.38±2.64)%～(5.56±4.74)% after incision 96～168h. Conclusions The expression characteristics of IL-10 in different expressive parts should be related to the wound age and morphometry of different expressive parts may be employed for determining the wound age. Related Articles | Metrics

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The study on expression of NF-κB in experimental brain contusion in rats TAO LU-YANG;CHEN XI-PING;DING MEI;ET AL. (SCHOOL OF MEDICINE;SUZHOU UNIVERSITY;SUZHOU 00;CHINA) 2004, 0(2): 73-76+8.  Abstract ( 1838 )   Objective To investigate the expression of NF-κB in different post-traumatic intervals and severity of brain injury. Methods Fifty-four brain tissue samples of slight (n=24), moderate(n=24) brain injury, sham(n=3) and control (n=3) of rat were examined by immunohistochemical staining, westernblot and RT-PCR. Results Up-regulating of NF-κB expression was found in tissues from traumatic brain injury compared with controls in early 1 hour after TBI, and reached peak at 24h and 48h and disappeared after 7 days. The expression of NF-κB mRNA has association with contusion severity. Conclusion NF-κB may benefit to the estimation of posttraumatic intervals of brain injury. Related Articles | Metrics

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A primary study on the gene expression profiling of human brain contusion by cDNA microarray ZHOU YI-WU;ZHANG YI-GU;LIU YAN;ET AL. ( FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA) 2004, 0(2): 77-80+1.  Abstract ( 2164 )   Objective To screen the differential expression of oncogenes and tumors suppressed genes(OTS genes) after human brain contusion by cDNA microrarray. Mathods The total RNAs isolated from normal and contusion human brain tissues were purified by Oligotex to obtain mRNAs. Both sources of mRNAs were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The probe from normal tissue and the contusion brain tissue were labeled with Cy3-dUTP and Cy5-dUTP respectively. The mixed probes were hybridized to the BioDoor Chip OTS-2.2S, a cDNA microarray which contains 227 oncogenes and tumors suppressed genes. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues. Results Among the 227 target genes, 3 genes including Human carcinoma associated HOJ-1(HoJ-1), Human KIAAOO65 gene,Human retinoblastoma related protein (p107) gene, showed distinct deference in expression level between the human brain contusion tissue and normal tissue. Conclusion The 3 genes in the brain contusion was significantly the differential expression by OTS 2.2S cDNA microarray. Further analysis of these genes will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine. Related Articles | Metrics

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Research on the application feasibility of HLA-DRB1 genotyping for forensic identification by oligonucleotide chip LI LI;LI CHENG-TAO;LIU YAN;ET AL. (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2004, 0(2): 81-84+8.  Abstract ( 2202 )   Objective Research on the application feasibility of HLA-DRB1 genotyping for forensic identification by oligonucleotide chip. Methods Population studies on HLA-DRB1 was carried out in a sample of 561 unrelated Chinese Han individuals using oligonucleotide arrays for genotype detection. Meanwhile, we explored the feasibility of typing HLA-DRB1 by DNA chip for forensic identification. Results No significant deviations from Hardy-Weinberg equilibrium could be found. The Observed heterozygosity(Ho) was 0.888. The Expected heterozygosity(He) was 0.902. The Polymorphic information content (PIC) was 0.893. The Average exclusion probability (PE) was 0.801. Pedigree analysis showed no deviation from Mendel′s Law. Conclusion The data and results of pedigree analysis and case application demonstrated that HLA-DRB1 typing by oligonucleotide probe arrays was a useful technique for paternity testing and individual identification. Related Articles | Metrics

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Polymorphism of STR loci D12S391?D18S865 in Wenzhou Han Population WU SHU-ZHEN;ZHANG HONG-QIN;BI YUN-TIAN (THE MOLECULAR BIOLOGY RESEARCH CENTER OF WENZHOU MEDICAL COLLEGE;WENZHOU 0;CHINA) 2004, 0(2): 85-87.  Abstract ( 2060 )   Objective To investigate the polymorphism and forensic efficiency values of STR loci D12S391?D18S865 Methods Population studies on D12S391 and D18S865 were carried out in a sample of 454 unrelated Wenzhou Han individuals using PCR followed by PAGE and silver stain detection. Results 11?7 alleles and 50?21 genotypes of D12S391?D18S865 were respectively observed among the 454 unrelated individuals. The genotype frequency matched the Hardy- Weinberg equilibrium, The heterozygotes(H) in D12S391?D18S865 were 0.797 4? 0.737 9, Power of Exclusion were 0.956 6? 0.907 7, polymorphism information content(PIC) were 0.826 0? 0.727 9 respectively. Conclusion D12S391 and D18S865 were high polymorphic loci and the frequencies were in accordance with Hardy-Weinberg equilibrium(P>0.5),so the two loci can be used in forensic medicine and in other genetic research areas. Related Articles | Metrics

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The genetic polymorphism of 7 Y-STR loci and haplotype in Tibetan Population ZHAO DONG;WANG BAO-JIE;DING MEI;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA) 2004, 0(2): 88-91.  Abstract ( 2151 )   Objective To investigate the genetic polymorphism of 7 Y-STR loci and haplotypes in Tibetan population. Methods The DNA samples from 67 Tibetan men were analyzed by PCR and the denatured PAGE followed by silver staining. Results 25 alleles were identified for 6 loci including DYS393 ,DYS389Ⅰ,DYS19, DYS390, DYS389Ⅱ and DYS392, 22 alleles were identified for DYS385, the frequency distributions were between 0.014 9～0.507 5, the DP value distributions were between 0.507 5～0.899 1, the DP value of DYS 385 was the highest. 51 haplotypes were identified for the YH6 system which consisted of the 7 loci, the DP value of this system was 0.988 7, the alleles for DYS393 and DYS389Ⅱ were few ,it followed that perhaps Tibetan population had met with bottleneck event in their evolution process. Conclusion The 7 STR loci above belong to loci of high discriminating ability, the haplotypes are highly polymorphic. Related Articles | Metrics

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The advance in study of sudden cardiac death mechanism WANG DIAN;YU XIAO-JUN(DEPARTMENT OF FORENSIC PATHOLOGY;MEDICAL COLLEGE OF SHANTOU UNIVERSITY;SHANTOU;0;CHINA) 2004, 0(2): 107-109+.  Abstract ( 1964 )   Incidence of sudden cardiac death(SCD) is increasing yearly all over the world,and SCD has become the largest killer of all diseases. Currently, lots of studies in this field have been launched worldwide. The review focuses on latest research result of its pathology , neuro-endocrine, electrophysiology, especially its mechanism of molecular biology. Related Articles | Metrics

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The advance of protection for hazard factor during autopsy WANG JI-FENG;CAO ZHE;CHEN XIN-SHAN (. HENAN INSTITUTE OF CRIMINAL SCIENCE && TECHNOLOGY; ZHENGZHOU 000;CHINA;. ANSHAN INSTITUTE OF CRIMINAL SCIENCE && TECHNOLOGY;;ANSHAN 00;CHINA;. DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL COLLE 2004, 0(2): 110-112.  Abstract ( 1901 )   Recently, the special characteristics of work with SARS require particular attention to the facilities, equipment, policies and procedures involved. In fact, an autopsy also subject prosectors and others to a wide variety of hazards, including bloodborne, aerosolized pathogens and others (for example SARS). Forensic pathologists and other persons in close proximity to an autopsy need personal protective equipment, fourthemore, laboratory procedure and facility design principles of biosafety should be established for the protection of all personnal involved in the work. Related Articles | Metrics

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Proceedings of human mitochondrial DNA Study and its applications to forensic medicine ZHOU XUE-PING;ZHANG WEI-JUAN;JIA ZHEN-JUN;ET AL. (WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA) 2004, 0(2): 113-115+.  Abstract ( 1956 )   The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the cytoplasm of the cell. Evidence of its existence first arose over 30 years ago. Now the field of the mitochondria is one of the fastest growing disciplines in biomedicine which is driven by fundamentally interesting questions. These questions are mainly about the way of mitochondria evolving and energy producing. In addition, what the consequences of mitochondrial genome mutations in diseases are? How program cell death is regulated? What happens to mitochondria when aging? These questions remain to be answered and the basic understanding of them will contribute to anthropological and forensic analysis, as well as therapy of many diseases. The following review has brought this question to notice by summarizing recent mitochondria research. Related Articles | Metrics

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Application of chi-square test and exact test in Hardy-Weinberg equilibrium testing HUANG DAI-XIN;YANG QING-EN (FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA) 2004, 0(2): 116-119.  Abstract ( 2933 )   This article review the application of chi-square test of various data handling methods and exact test in Hardy-Weinberg equilibrium testing of human genetic marker in population genetics. The importance of HWE-exact test in multiallelic system was emphasized, especially in the study of forensic VNTR and STR typing. Related Articles | Metrics

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Progress and prospects of HLA genotyping technology LI CHENG-TAO (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2004, 0(2): 120-123.  Abstract ( 1914 )   Over a long period of time, studies on HLA structure and function have been the research hotspots. for it is very important to understand the essential of life science and disease mechanism. With the rapid development of molecular biology, HLA typing makes great progress. It has changed from traditional serological typing to DNA-based typing. More and more HLA genotyping methods have been developed and applied. In this essay, the author reviewed and appraised all kinds of HLA genotyping tech niques and introduced two new techniques. Related Articles | Metrics