A primary study on the gene expression profiling of human brain contusion by cDNA microarray

Abstract

Abstract: Objective To screen the differential expression of oncogenes and tumors suppressed genes(OTS genes) after human brain contusion by cDNA microrarray. Mathods The total RNAs isolated from normal and contusion human brain tissues were purified by Oligotex to obtain mRNAs. Both sources of mRNAs were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The probe from normal tissue and the contusion brain tissue were labeled with Cy3-dUTP and Cy5-dUTP respectively. The mixed probes were hybridized to the BioDoor Chip OTS-2.2S, a cDNA microarray which contains 227 oncogenes and tumors suppressed genes. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues. Results Among the 227 target genes, 3 genes including Human carcinoma associated HOJ-1(HoJ-1), Human KIAAOO65 gene,Human retinoblastoma related protein (p107) gene, showed distinct deference in expression level between the human brain contusion tissue and normal tissue. Conclusion The 3 genes in the brain contusion was significantly the differential expression by OTS 2.2S cDNA microarray. Further analysis of these genes will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine.

Key words: cDNA microarray, gene expression profiling, brain contusion, oncogene, tumors suppressed gene

ZHOU YI-WU;ZHANG YI-GU;LIU YAN;ET AL. ( FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA). A primary study on the gene expression profiling of human brain contusion by cDNA microarray[J]. , 2004, 0(2): 77-80+1.

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A primary study on the gene expression profiling of human brain contusion by cDNA microarray

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