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28 June 2004, Volume 20 Issue 3 Previous Issue    Next Issue
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tudy on the changes of electrocardiogram and ultrastructural in heroin dependence in rats LIU XIAO-SHAN;CHEN YU-CHUANG;LI ZHAO-HUI;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CEN-TER OF ZHONGSHAN UNIVERSITY;GUANZHOU000;CHINA) 2004, 0(3): 129-132+.  Abstract ( 2721 )   Objective To study the changes of electrocardiograms(ECG)and myocardial ultrastructure in heroin dependence in rats,in order to reveal the mechanisms of the myocardial injury by heroin.Meth-ods Establish heroin addict model in SD mice,investigate the changes in electrocardiograms,HE staining and myocardial ultrastructure.Results The electrocardiograms of the addict group had prominently changes,main expressions:heart rate decreased,P wave and T wave amplitude reduced and duration in-creased,S-T reduced and duration increased,QT interval prolongation,these changes indicated that my-ocardium had been injured,myocardial ischemia,ventricle function declined.These difference was signifi-cant(P<0.05)between before inject heroin and after inject heroin.Transformations in the ultrastructure:nuclear concentrate,reduce,nuclear membrane shrink,chromatin agglutinate,mitochondria cristal had dis-order formation,disappeared or hollowed,these indicated that heroin could cause pathological changes in myocardial ultrastructure.Conclusion Above-mentioned changes indicated that heroin can injure myocardi-um,and the changes of myocardial ultrastructure suggested that myocardial apoptosis may be one of the mechanisms of the myocardial injury by heroin. Related Articles | Metrics

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The study of the major sarcosaphagous flies in hohhot CAI JI-FENG;TAO TAO;DONG JIAN-GUO;ET AL.(.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA;.DEPARTMENT OF PATHOLOGY;SCHOOL OF PRECLINICAL MEDICINE;SHANGHAI SECOND MEDICAL UNIVERSITY;SHANGHAI000;CHINA) 2004, 0(3): 133-135.  Abstract ( 2033 )   Objective In order to determine the major species of sarcosaphagous flies and their regular activity on carcass in Hohhot district.Methods Six rabbits were killed and placed outdoors at different time from July to October in Hohhot district.Some species of sarcosaphagous flies that appeared the ca-davers were observered and identified.Results It showed that there are10main spieces belonging to3families and8genera were from Diptera,including Musa domestica domestica Linnaeus,Musa domestica vicina Maequart,Ophyra capensis(Wiedemann),Hydrotaea armipes(Fall.),Muscina stabulans(Fall.)from Museidae;Lucilia sericata(Meigen),Chrysomya megacephala(Fabricius),Lucilia cuprina(Wiedemann)from Calliphoridae;Boettcherisca peregrina(Robineau-Desvoiy),Parascarcophaga crassipalpis(Maequart)from Sacrophagidae.Besides,it showed obvious regulations that different species of sarcosaphagous flies ap-peared on carcass at different postmortem interval and the first part of carcass that sarcosaphagous flies appeared on.Conclusion It may be useful for estimating postmortem interval in Hohhot district. Related Articles | Metrics

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Immunohistochemical study of Cx43 dephosphorylation in human left ventricular myocardium suf-fered by acute ischemia ZHANG SHI-WEI;LIU SHI-XIN;DENG LI-BIN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COL-LEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000;CHINA) 2004, 0(3): 136-139+.  Abstract ( 1851 )   Objective This study was performed to detect the phosphorylation state of Cx43in human left ven-tricular myocardium among sudden deaths caused by acute myocardial ischemia(AMI)especially sudden coro-nary death(SCD)and control groups.And then evaluate the significance of these findings in diagnosing the early pathological changes of acute myocardial ischemia.Methods Immunohistochemistry(IHC)SP techniques were adopted to detect the phosphorylation state of Cx43in the left ventricular myocardium samples of45deceased,which classified as groupⅠ-SCD,groupⅡ&Ⅲ(other two groups of AMI)and GroupⅣ&Ⅴ(two control groups,sudden death caused by lethal acute cranio-cerebral injury or pathologic intracranial hemorrhage).In addition,we selected anti-Pan-Cadherin(construction protein of adherent junctions on the intercalated disc)and PHA-E+L/Bio,to detect the integration of myocardial mechanical coupling and membranes(applying affinityhisto-chemistry,AHC)respectively.Results⑴Phosphorylated Cx43positive staining was almost invisible in GroupⅠ,ⅡandⅢor scattered in sarcoplasm in few samples;but it was assembling at the IDs clearly in groupⅣandⅤ.⑵Strongly positive staining of Pan-Cadherin could be observed at the IDs and⑶integrated myocardial mem-branes were found in all samples.Conclusion These findings suggested that compared with the control groups,the integration of myocardial mechanical coupling and membranes did not alter in AMI.But Cx43,the key pro-tein of electrical coupling on myocardial gap junctions,occurred dephosphorylation remarkably in AMI.Thus ap-plying IHC techniques to detect the Cx43dephosphorylation in human left ventricular myocardium maybe useful to recognize the onset of arrhythmia in AMI,especially in SCD whose myocardium without apparent morphologi-cal changes. Related Articles | Metrics

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Study on relationship between changes of geometry parameters in human spleen nuclei and the postmortem interval SHU XI-JI;LIU-LI-JIANG;HU JIA-WEI;ET AL.(.SCHOOL OF MEDICINE﹠LIFE SCIENCES;JIANGHAN UNIVER-SITY;WUHAN00;CHINA;.THE DEPARTMENT OF PUBLIC SECURITY;WUHAN00;CHINA;.FACULTY OF FOREN-SIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCI 2004, 0(3): 140-142.  Abstract ( 2274 )   Objective Using computer image-analyze technique(CIAT)to study changes of geometry pa-rameters in human spleen nuclei and seek a new experimental method to deduce the estimation the post-mortem interval(PMI).Methods31cadavers that known accurate PMI,sampled and smeared respectively every hour within the first36hours after death,fixed with cold Carony fixation,stained by Feulgen-van's method,and measured5geometry parameters using the image-analyze instrument including Area(A),Mean-Dia(MD),Average Diameter(AD),perimeter(P),Index of density(ID).Results A,MD,AD and P in the human spleen nuclei have no correlation with the PMI.But ID rose regularly with the prolongation of PMI in36hours.There was a definite correlation between ID and the PMI,r=0.983,linear regression equation with PMI(hours)as the dependent variable was calculated for ID.Conclusion Geometry parame-ter ID was proved to be preferable indexes for estimation of PMI in36hours. Related Articles | Metrics

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Concentration changes of amylase in vitreous humor of acute pancreatitis associated with different postmortem interval in rabbits LI XING-BIAO;ZHANG HAI-YAN;SHAO LI-MING(DEPARTMENT OF FORENSIC MEDICINE;WENZHOU MEDICAL COLLEGE;WENZHOU0;CHINA) 2004, 0(3): 143-144.  Abstract ( 2209 )   Objective To investigate concentration changes of amylase in rabbits vitreous humor of acute pancreatitis associated with postmortem different interval.Methods To induce animal model of acute pan-creatitis with sodium taurocholate,observed concentration changes of amylase after different postmortem in-terval,then compared with normal.Results The concentration of amylase in vitreous humor of rabbit24h after death were related to postmortem interval significantly;The concluded formulae of the relationship between postmortem interval and amylase concentration is y=8.7420+0.7699x-0.0083x2(R2=92.62792,F=14.89734,P=0.001).Conclusion Concentration changes of amylase in vitreous humor of acute pancreatitis associated with postmortem interval may provide a new sensitive and objective method for the forensic early injury time estimation. Related Articles | Metrics

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Identification of grades division to diminished criminal responsibility in mental disorder offender CAI WEI-XIONG;SHAO YANG;GUAN WEI;ET AL.(.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI000;CHINA;.SHANGHAI MENTAL HEALTH CENTER;SHANGHAI0000;CHINA) 2004, 0(3): 145-149.  Abstract ( 3047 )   Objective To explore the basis to discriminate diminished criminal responsibility.Methods Chi-square test,one-way ANOVA test,factor analysis and stepwise discriminant analyses were conducted for92cases which were divided into three groups according to the degree of diminished criminal respon-sibility.Results There were significant differences in the type of crime and rating score among three groups.The factor1reflecting the premeditation and preparation before crime was the most important of all factors.Realistic motivation,time selectivity of offending,emotion during the crime,understanding the nature of the behavioral,impairment of life ability and impairment of reality testing were the discriminat-ing factors of grades division in diminished criminal responsibility.Conclusion The diminished criminal responsibility in mental disorder offender could be divided into three grades. Related Articles | Metrics

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The creation of an instrument to determine competency to stand trial ZHANG QIN-TING;ZHANG WEI;HUO KE-JUN;ET AL.(.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA;.WEST CHINA HOSPITAL;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2004, 0(3): 150-151+.  Abstract ( 2248 )   Objective To create an instrument to determine the mental disorder offenders' competency to stand trial,which is according with the Chinese legal system.Methods Integrating the foreign instruments and the Chinese criminal jurisprudence,and combing the authors' forensic psychiatric experience,the research team created an instrument which was called Competency to stand trial Determining Instrument firstly,then used the instrument in practice,in the end the validity and reliability of the instrument were inspected,and through an diagnostic test,the feasibility of the instrument was evaluated.Results Split-half and homogeneity reliability of the instrument is0.9366and0.9213respectively,the correspondence of the conclusion between the instrument and the expertise is0.704.Except the sensitivity is0.6097,all the other diagnostic index are over0.80.Con-clusion The Competency to stand trial Determining Instrument is feasible. Related Articles | Metrics

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A study of mental health issues in the objects of substance abstinence who got labor education and rehabilitation GUAN WEI;ZHANG QIN-TING;JI JIAN-LIN(.ZHONGSHAN HOSPITAL;FUDAN UNIVERSITY;SHANGHAI000;CHINA;.THE INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI000;CHINA) 2004, 0(3): 152-154.  Abstract ( 2040 )   Objective To investigate the personality and mental health of the objects of substance(caf-feine,cocaine,hallucinogens and so on)abstinence who got labor education and rehabilitation.Methods258male objects and250female objects of substance abstinence selected randomly from institutions of labor education and rehabilitation were assessed with MMPI and SCL-90.Results MMPI tests indicated the personality abnormality in the objects.SCL-90tests manifested the mental health abnormality of them compared with Chinese models,especially in the female objects.Conclusion More attention should be paid on the mental health of the objects of substance abstinence. Related Articles | Metrics

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Study of DNA identification in burned bones YE JIAN;JI AN-QUAN;ZHAO XING-CHUN;ET AL.(INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF PUBLIC SECURITY;BEIJING000;CHINA) 2004, 0(3): 155-159.  Abstract ( 2328 )   Objective For the purpose of solving a problems of DNA testing of burned bones.Methods We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammoni-um bromide(CTAB)lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purifi-cation using the DNA IQTMSystem.Results The methods were found to be effective in removing the PCR inhibitors from the burned bone.Then the extracted DNA was successfully genotyped by using the flores-cence labeling STR multiplex method.Conclusion The results of this research will assist forensic scien-tists in the identification of DNA from victims whose bodies underwent significant trauma or burning,pre-cluding the utilization of traditional forensic DNA identification techniques. Related Articles | Metrics

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A study of genetic polymorphism of the STR locus D7S2846、D19S400 and D18S535 in Han popula-tion in Wenzhou ZHANG HONG-QIN;WU SHU-ZHEN;BI YUN-TIAN(MOLECULAR BIOLOGY RESEARCH CENTRE OF WENZHOU MEDICAL COL-LEGE;WENZHOU0;CHINA) 2004, 0(3): 160-161.  Abstract ( 2045 )   Objective To study genetic polymorphism of D7S2846、D19S400and D18S535in Han population in Wenzhou.Methods DNA was extracted with chelex-100method from EDTA-blood samples of194unrelated individuals in Wenzhou and amplified with PCR technique.The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.Results6alleles and15genotypes of D7S2846、10alleles and36genotypes of D19S400、8alleles and26genotypes of D18S535was observed.The heterozygosities of the three loci are0.644,0.724and0.772;The power of discriminating are0.854,0.940and0.938.Conclusion The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium(P>0.05),so the three loci can be used in forensic medicine and in other genetic researches. Related Articles | Metrics

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A genetic study of 15 STR loci in Chinese E Wen-ke population HUANG YU-LI;GU MMING-BO;WANG JIAN;ET AL.(CRIMINAL SCIENCE&TECHNOLOGY INSTITUTE OF HEI-LONGJIANG PROVINCE;HARBIN000;CHINA) 2004, 0(3): 162-163+.  Abstract ( 1922 )   Objective To investigate the genetic polymorphisms of15STRs loci in Chinese E Wen-ke population.Methods DNA samples from99unrelated individuals in Chinese E Wen-ke population were screened by Power Plex16System and ABI3100Genetic Analyzer.Results The genotype frequencies of these15STR loci meet the Hardy-Weinberg expectation.The Matching probability of15STRs loci were between0.0205-0.1733,discriminating power(DP)at0.8267-0.9795,heterozygosity(Ho)at0.6061-0.9091,power of exclusion(PE)at0.4038-0.7690,polymorphism information content(PIC)at0.5985-0.8734.The total DP of15STRs loci is0.9999999999998.Conclusion Our results showed that the15STRs loci of Power Plex16System are valuable STR loci genetic marker system having high DP and are useful in forensic case work in Chinese E Wen-ke population. Related Articles | Metrics

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A genetic study of 15 STR loci in Chinese East Mongolian population DU QING-XIN;WANG JIAN;HUANG YU-LI;ET AL.(CRIMINAL SCIENCE&TECHNOLOGY INSTITUTE OF HEI-LONGJIANG PROVINCE;HARBIN000;CHINA) 2004, 0(3): 164-166.  Abstract ( 1925 )   Objective To investigate the genetic polymorphisms of15STRs loci in Chinese East Mongo-lian population.Methods DNA samples from105unrelated individuals in Chinese East Mongolian popu-lation were screened by Power Plex16System and ABI3100Genetic Analyzer.Results The genotype fre-quencies of these15STR loci meet the Hardy-Weinberg expectation.The Matching probability of15STRs loci were between0.0084～0.2169,discriminating power(DP)at0.7831～0.9916,heterozygosity(H)at0.5619～0.9231,power of exclusion(PE)at0.4490～0.8444,polymorphism information content(PIC)at0.5438～0.9178.The total DP of15STRs loci is0.9999999999998.Conclusion Our results showed that the15STRs loci of Power Plex16System are valuable STR loci genetic marker system having high DP and are useful in forensic case work in Chinese East Mongolian population. Related Articles | Metrics

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Progression of the manner of cardiomyocyte death and its potential significance in forensic medicine FENG XIANG-PING;WU WEI;CHEN XIN-SHAN(DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000;CHINA) 2004, 0(3): 175-177+.  Abstract ( 2257 )   The manner of cell death is a hotspot of medical researchers.Apoptosis and necrosis were considered as two manners of cell death in the past.But recently a new manner of cell death—oncosis is gradually accepted by the pathologists.Oncosis is different from apoptosis in morphologic,mechanism and the role in cardiovascular diseases.In this paper,the progression of the research about manner of the cardiomyocyte death and its signifi-cance in forensic medicine in recent years was reviewed. Related Articles | Metrics

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Mitochondrial apoptotic signaling pathway in neurons following brain injury induced by hypoxia ZHNAG KUI-MING;LIAO ZHI-GANG(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2004, 0(3): 178-182.  Abstract ( 1839 )   Impairment of neuronal mitochondria following hypoxia of brain not only result in nerve cell's energy-deprivation and dysfunction,mitochondria also play key roles in apoptosis of neurons.A central step being the release of cytochrome c(cyt c)across the outer mitochondrial membrane into the cytoplasm through opening of the mitochondrial permeability transition pore.Releasing of cytochrome c induce to downstream consequences of specific caspase activation.The antiapoptotic and proapoptotic members of the Bcl-2family regulate mitochondrial activities relevant to apoptotic signaling by influencing the realaseing of cyt c. Related Articles | Metrics

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Study of toxicology of strychnos WANG QI-WEI;LIU LIANG;HUANG GUANG-ZHAO(FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN000;CHINA) 2004, 0(3): 183-184.  Abstract ( 2220 )   Because of its officinal value,strychnos is widely used by clinic and individual.Since toxic dose and thera-peutic dose are very close,strychnos poisoning cases are frequently reported.In this paper the chemical component,tox-ic dose,mechanisms of toxicity,poisoning symptom and pathological changes after strychnos poisoning are reviewed. Related Articles | Metrics

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The study developments about changes of methamphetamine-induced genes' transcriptions and translations YANG JING(WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU00;CHINA) 2004, 0(3): 185-188.  Abstract ( 2242 )   The basic changes are to transform the levels of many genes' transcriptions and translations when methamphetamine is injected into the organism.Those genes enclose four classes:genes intermediating the dam-ages or death of neurons,genes involving circadian rhythms of activity,genes concerning the abnormality of be-haviors and some genes difficult to be classified.The transformations of the transcriptions or translations of these genes cooperate to produce many clinic syndromes of methamphetamine-addictors.Moreover,the study of these genes can provide testimonies to forensic identification. Related Articles | Metrics