Abstract: Objective For the purpose of solving a problems of DNA testing of burned bones.Methods We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammoni-um bromide(CTAB)lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purifi-cation using the DNA IQTMSystem.Results The methods were found to be effective in removing the PCR inhibitors from the burned bone.Then the extracted DNA was successfully genotyped by using the flores-cence labeling STR multiplex method.Conclusion The results of this research will assist forensic scien-tists in the identification of DNA from victims whose bodies underwent significant trauma or burning,pre-cluding the utilization of traditional forensic DNA identification techniques.

Key words: cetyltrimethyl ammonium bromide(CTAB), collagen aged bones and burned bones DNA, DNA IQTMsystem, purification, forensic DNA examination, florescence labeling STR multiplex