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28 August 2004, Volume 20 Issue 4 Previous Issue    Next Issue
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ABO Genotyping by Duplex Amplification and Oligonucleotide Arrays Assay LI LI;LI CHENG-TAO;LI RONG-YU;ET AL. (. INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA;.SHANGHAI BIOSTAR GENECHIP RESEARCH INSTITUTE;SHANGHAI 000;CHINA) 2004, 0(4): 193-196+.  Abstract ( 2340 )   Objective ABO genotyping for forensic identification by oligonucleotide chip. Methods Oligonucleotide microarrays which could detect 3 different SNPs in exon 6 and exon 7 for ABO genotyping were used. Population studies on ABO was carried out in a sample of 115 unrelated Chinese Han individuals. The method was also applied to cases. Results The technique could identify 6 genotypes of ABO system. According to the results of population studies, no significant deviations from Hardy-Weinberg equilibrium could be found. The observed and expected heterozygosities were 0.591 and 0.616 respectively. The polymorphic information content was 0.544. The average exclusion probabilities in buos and trios was 0.188 and 0.344 respectively. The discrimination power is 0.777. Conclusion The data and case application demonstrated that ABO typing by oligonucleotide probe arrays was a useful technique for paternity testing and individual identification. Related Articles | Metrics

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The Study on HLA-Cw Polymorphism from Xi′an Han Population by PCR-sequence Specific Oligonucleotide Probe ZHANG HONG-BO;LAI JIANG-HUA;ZHAO JUN-HAI;ET AL. (THE NATIONAL LABORATORY OF THE MINISTRY OF HEALTH FOR FORENSIC SCIENCES;XI′AN JIAOTONG UNIVERSITY;XI'AN 00;CHINA) 2004, 0(4): 197-199.  Abstract ( 2041 )   Objective To determine the HLA-Cw allele and genotype frequencies from Xi'an Han population and obtain genetic data. Methods The results of HLA-Cw typing for 130 randomly selected from Xi'an Han population were obtained by using the PCR-Sequence Specific Oligonucleotide Probes (SSOP). Results In this investigation, 16 alleles were detected among 130 unrelated individuals with frequencies from 0.007 7 to 0.158 8 and HLA-Cw*01?03?07 were the most common HLA-Cw alleles. We have made a survey of HLA-Cw alleles frequencies in Xi'an Han Population, with blank frequency being lowered to 0.018 2. Conclusion The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by Hi-square test. The frequency data can be used in forensic and paternity tests, transplant matching and anthropology. Related Articles | Metrics

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Genetic Polymorphism of two STR Loci D2S1399 and D5S2500 in Eastern Chinese Han Population XIA SHUI-XIU;GAO YU-ZHEN;BIAN SHI-ZHONG;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;MEDICAL SCHOOL OF SOOCHOW UNIVERSITY;SUZHOU 00;CHINA) 2004, 0(4): 200-201+.  Abstract ( 2003 )   Objective To obtain the genetic polymorphism data of two STR loci D2S1399 and D5S2500 in Eastern Chinese Han population. Methods Blood samples or buccal swabs of unrelated Han individuals living in eastern China were analyzed using PCR-nature polyacrylamide gel electrophoresis-sliver staining method. Results 11 alleles of D2S1399 and 9 alleles of D5S2500 were observed in the samples respectively, the observed heterozygosity (Ho) values, the discrimination power (DP) values and the power of exclusion(PE) values of D2S1399 and D5S2500 is 0.745 and 0.807, 0.958 and 0.917, 0.554 and 0.643, respectively. Conclusion The result showed that D2S1399 and D5S2500 were highly informative loci and suitable for forensic application. Related Articles | Metrics

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A sort of Meliorative Method for Testing DNA from Sperm Smear SUN YAO-DONG;REN HONG;CHEN YUE-YONG (INSTITUTE OF CRIMINAL SCIENCE;PUBLIC SECURITY BUREAU OF YANCHENG CITY;YANCHENG 00;CHINA) 2004, 0(4): 202-204.  Abstract ( 2138 )   Objective To establish an effective method for testing DNA from the sperm smear. Methods We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA.The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit. Results Although there were a few sperm cells on the slide,it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction. Conclusion The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case. Related Articles | Metrics

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A Study of the Expression of Fas after Fluid Percussion Brain Injury in Rats PAN HONG-FU;WANG YE;CHEN XIAO-GANG;ET AL.( WEST CHINA COLLEGE OF BASIC MEDICINE AND FORENSIC MEDICINE OF SICHUAN UNIVERSITY;CHENGDU 00;CHINA) 2004, 0(4): 205-207+.  Abstract ( 2187 )   Objective To study the pathologic diagnosis and the injury time estimation in light closed encephalon injury. Methods Mice were hurt by fluid percussion, and were killed at 15, 30 min, 1, 3 , 6, 12 h, 1, 4, 7,14 d respectively after injury. The expression of Fas-L in the cerebral cortex, thalamus, and hippocampi was detected by immunohistochemistry and the results were assessed by image analysis system. Results It is showed that the expression of Fas-L could be detected in 1 h after injury, and increased significantly in three hours, and it reached apex 12 h after injury, and decreased gradually four days after injury, and returned normal 14 days after injury. Conclusion This research demonstrated that Fas-L mediated apoptosis appeared not only around brain trauma but also in the brain tissue far away from the traumatic area. It indicted that the expression of Fas-L is a useful target for diagnosis of early brain injury; the regularity of Fas-L expression could be used as one of indication to date the time of brain injury. Related Articles | Metrics

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Comprehensively Analysis the Correlation between the Height of a Person and the Length of His/Her Handprint HU XIANG-YANG;YAO HUI-FANG;LIN JIAN-HUI (HUBEI POLICE OFFICER COLLEGE;WUHAN 00;CHINA) 2004, 0(4): 208-211.  Abstract ( 1895 )   Objective Comprehensively analysis the correlation and the law of variation between the height of a person and the length of his/her handprint. Methods Collecting handprint samples of those people of different age and sex from different area all of our country, and adopt the regressive analysis method to study these samples. Results A sum of useful data and regressive equation were obtained. Conclusion The correlation between the height of a person and the length of his/her handprint is obviously, the approximate height of a person can be reckoned according to the length of his/her handprints. Related Articles | Metrics

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Preliminary Study on the Applying Value of Two Measurements for Bone Age in the Cases of Minors ZHANG ZHEN;LI KAI;YU RONG-JUN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE;XI′AN JIAOTONG UNIVERSITY;XI′AN 00;CHINA) 2004, 0(4): 212-214.  Abstract ( 1916 )   Objective To research the connection of the minor′s bone age and chronological age and age for penal responsibility, to discuss the application of Gu′s Atlas and Li Guozhen′s Percent Numeration to measure the bone age in the cases of immature criminals and the applying value. Methods 33 healthy youths,12-19 years old, were selected, whose left wrists were photographed with X-rays. According to the radiographic appearance of the maturity indicators, the bone age of every person was determined respectively by the Gu′s Atlas and Li Guozhen′s Percent Numeration, and then compared with his/her chronological age. CMH test and Pearson test were used to evaluate precision and accuracy on concluding the immature age for penal responsibility. Results Li Guozhen′s Percent Numeration was more precise than Gu′s Atlas on extrapolating the chronological age of the youth, P<0.005. On extrapolating the age for penal responsibility, the accuracy of the former was 90.91%, and the latter was 78.79%, but the statistic result did not show significant difference (P>0.1). Conclusion Both measurements can apply in determining the criminal cases of immature youths. Related Articles | Metrics

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Reasons why the Quality of Medico-legal Autopsy in the Medical Tangle Varies from two Areas LIU YAN;ZHU CHUAN-HONG;HUANG GUANG-ZHAO;ET AL. (. DEPARTMENT OF MEDICOLEGAL;TONGJI MEDICAL COLLEGE;HUAZHONG SCIENCE AND TECHNOLOGY UNIVERSITY;WUHAN 000 CHINA;. INSTITUTE OF FORENSIC SCIENCE;WUHAN PUBLIC SECURITY BUREAU;WUHAN 00 CHINA) 2004, 0(4): 215-217.  Abstract ( 2117 )   Objective To study reasons that the quality of medico-legal autopsy in the medical tangle varies from different area. Methods Collecting the cases of medical tangle in two medico-legal agencies, then counting percent of classes on the ten key-points, analyzing the data of the cases by chi-square test and t-test. Results It is indicated that the applied methods and standards of the two agencies are different. There are more different in seven keypoint of medicolegal autopsy by chi-square test. Conclusion Six key-points are found to be more important to medico-legal appraiser, standardization of forensic autopsy, standardization of picking up specimen from the body, diagnosis standardization of the cause of death, consultation system and standardization of writing documents on medico-legal autopsy. Related Articles | Metrics

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Study of Affecting Factors of Mentally Prisoner′s Competency to Serve a Sentence HUANG FU-YIN;LU CHENG-RONG;ZHANG QIN-TING;ET AL. (INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2004, 0(4): 218-220.  Abstract ( 1994 )   Objective Explore the affecting factors of mentally prisoner′s competency to serve a sentence(CSS), establish the base of quantitive study of CSS. Methods Firstly, the researchers compile a questionnaire named legal-psycho ability of competency to serve a sentence questionnaire, then the researchers scaling the object with RTHD, and ask all object complete the questionnaire. there a hypothesis, that the object who are cured in cured ward is incompetent to serve a sentence(ISS), and the other who are stay in rehabilitated ward is competent to serve a sentence(CSS). Results There are 185 object admitted the study, the ISS group the CSS group have significance between psychiatric and legal aspects. Conclusion At the influence of psychiatric symptoms, the mentally prisoner′s competency to serve a sentence had been impaired, and they should been transferred from prison to hospital. Related Articles | Metrics

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Expressions of NOS Isoforms and Roles of NO during Skin Wound Healing GUAN DA-WEI;ZHAO RUI;DU YU (SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA) 2004, 0(4): 244-246.  Abstract ( 2202 )   Skin wound healing is an organized process for keeping cutaneous integrity, which needs the complicated interaction between inflammatory cells and biochemical mediators. Nitric oxide synthase (NOS) isoforms , as important factors during skin wound healing, synthesize nitric oxide (NO) which may be involved in the whole event of skin wound healing and play important roles in cell proliferation, differentiation and apoptosis, angiogenesis, matrix deposits and remolding. Furthermore, NO also exerts effects in some cutaneous diseases. In the present paper, the expressions of NOS isoforms and the roles of NO during skin wound healing were reviewed with references to the advances in the studies on skin wound healing. It is suggested that nitric oxide synthases, play significant parts during skin wound healing, which is worthy of further investigation. Related Articles | Metrics

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The Damage of Cardiovascular System in Heroin Abuses LIU XIAO-SHAN;CHEN YU-CHUANG;LI ZHAO-HUI;ET AL. ( DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF ZHONGSHANG UNIVERSITY;GUANZHOU 000;CHINA) 2004, 0(4): 247-249.  Abstract ( 2472 )   The heroin abuses can seriously damage human body system, among them the damage of cardiovascular system is various. In this paper those damages involved heart rate, blood pressure, electrocardiogram, heart function, blood circulation, the changes of some material inside, and complications of cardiovascular system are reviewed. Related Articles | Metrics

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Gap Junctions, Connexins and Sudden Death Caused by Coronary Heart Disease ZHANG SHI-WEI;CHEN XIN-SHAN;LIU SHI-XIN;ET AL. (FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN;000;CHINA) 2004, 0(4): 250-252.  Abstract ( 2129 )   Gap junctions construct hydrophilic trans-membrane channels which adjust the intercellular communication of chemistry and electricity. In the heart, individual cardiac myocytes are linked by gap junctions. These junctions form low resistance pathways along which the electrical impulse flows rapidly and repeatedly between all the myocardium, ensuring their synchronous contraction. In recent years, some researchers have found that connexins, the protein molecules of gap junction channels, are reduced in number or redistributed from intercalated disks (ID) to lateral cell borders in a variety of cardiac disease, especially in ischemic heart disease. The gap junction remodeling is considered to be arrhythmogenic. These findings will lead us to a new realm in the diagnostic of sudden death caused by coronary heart disease. Related Articles | Metrics

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Application of the age-associated injure in mitochondrial DNA LOU DI-DONG;YU CHUN-YING;YANG QING-EN (FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA) 2004, 0(4): 253-255.  Abstract ( 2065 )   Nowadays, the injury in human mitochondrial DNA(mtDNA) is well known to accumulate in various tissues with age. It′s significant to further investigate and then apply it to estimation of the age at parenchymas. Related Articles | Metrics

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Physical Injury and Mental Injury ZHAO HU (DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE;SHANTOU UNIVERSITY;SHANTOU 0;CHINA) 2004, 0(4): 256-258.  Abstract ( 2050 )   Physical and mental injury exist as relative aspect of human injury. They have different medicine and legal feature, as well as mutual dependence and conversion. Physical and mental injury not only present respective character in the classification, but also have difference in specific basis of rating. Related Articles | Metrics