Journal of Forensic Medicine

›› 2005, Vol. 0 ›› Issue (1): 6-8.

• 论文 • Previous Articles     Next Articles

DNA Samples Preparation from Single Cell and its Application in Sensitivity Test

DENG JIAN-QIANG1, SHI MEI-SEN2, YING BING-WU2, ET AL. (1.INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063,CHINA; 2. INSTITUTE OF FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041 CHINA)   

  • Online:2005-02-25 Published:2005-02-28

Abstract: Objective To establish a reliable, exact and practical method to prepare DNA samples for sensitivity-test purposes. Methods The micromanipulation method was employed to prepare exact quantity DNA samples used to study the sensitivity of Profiler Plus?誖Kit-ABI PRISMTM 310 system. Results We succeed in establishing a micromanipulation method to prepare groups of DNA samples ,which contain 1-11 cells in turn, and also succeed in using them to study the sensitivity of Profiler Plus?誖Kit-ABI 310 system. Conclusion The method we have established is proved to be a reliable, exact and practical way to prepare DNA samples for sensitivity-test purposes.

Key words: sensitivity test, DNA sample preparation, single cell