Abstract: Objective For the purpose of more accurate and rapid analysis of aconitine in medicine wine, a reversed-phase High Performance Liquid Chromatography (HPLC) method was developed. Methods Standard aconitine was added to the blank wine when the wine sample was pretreated. The pretreatment method of samples, the linear range, the precision, the recoveries in the plasma were investigated by using of white wine plasma spiked with standard aconitine. Results The linear range was 0.45～9.0 μg·mL-1, r=0.998 8. The detective limit was 0.45 μg·mL-1, The intra and inter-day precision of assay for aconitine were less than 3.1% and 4.7% (n=5) in wine respectively. The recoveries of aconitine were more (97.3±2.8)% in medicine wine. The HPLC method has been used to investigate the concentration of aconitine in one forensic medicine case. Conclusion The HPLC method of quantitative analysis aconitine is rapid and sensitive. It is only in the 2.0 h that determination of aconitine in the medicine wine.

Key words: forensic toxicological analysis, aconitine, medicine wine, high-performance liquid chromatography