Abstract: Objective Research on the application feasibility of SNP genotyping for forensic identification by microarrays. Methods Oligonucleotide microarrays which could detect 31 different SNPs were used. Population studies on 31 SNP loci was carried out in a sample of 109 unrelated Chinese Han individuals using oligonucleotide microarrays for genotype detection. The method was also applied to cases. Results No deviations from Hardy-Weinberg equilibrium could be found at the 31 SNP loci 4 loci were medium informative and 27 were high informative. The combination discrimination power (CDP) of the 31 optimal informative SNPs was 0.999 999 999 997 9. The matching probability was 2.13×10-12. The average exclusion probability in duos and trios were 0.960 9 and 0.997 0 respectively. Conclusion The data and case application demonstrated that SNP typing by oligonucleotide probe microarrays was a useful technique for paternity testing and individual identification.

Key words: forensic science, genotyping, SNP, microarray