Preliminary Application on Test Paper of Gold-labelled Antibody against Fibronectin EⅢA Splicing Variant

Abstract

Abstract: Objective To develop the test Paper of gold-labelled antibody against fibronectin EⅢA splicing variant, which can be available in forensic wound interval estimation. Methods Two sensitive antibodies were compared with enzyme link immunoabsorband assay (ELISA). After colloid gold labeled, the effects of the two antibodies were tested by methods of Dot immunogold filtration assay and gold immunochromatography assay, respectively. The test paper was finally appraised by applied in experimental skin injury in rats. Results On the test paper, detected line appeared in three hours wound age group, and then the darkness of positive staining became darker with injury time prolonging, while control normal skin cannot found to be positive staining. Conclusion The gold-labeled test paper can be useful in estimation of wounding interval in forensic science.

Key words: fibronectin, alternative splicing, extra domain, EⅢA, wound interval estimation, colloid gold

LIU NING-GUO;CHEN YI-JIU;LU REN-QUAN;HUANG XIAO-HUA;ZHANG JIAN-HUA (. INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;CHINA;.SHANGHAI METERIA MEDICA BIOENGIERING INSTITUTE;SHANGHAI 0000;CHINA). Preliminary Application on Test Paper of Gold-labelled Antibody against Fibronectin EⅢA Splicing Variant[J]. , 2006, 0(2): 81-83.

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Preliminary Application on Test Paper of Gold-labelled Antibody against Fibronectin EⅢA Splicing Variant

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