Expression of EDA-fibronectin in Injured Human Skin Fibroblast and its Potential Application in Estimation of Wound Healing

Abstract

Abstract: Objetive To study the expression of EDA-FN variant in injured human skin fibroblast and its relation to wound healing.Methods Human skin fibroblast cells were separated from human skin and cultured and monoclonal antibody to fibronectin [IST-9] was applied to amplify target segments.Results Trace amount of EDA-FN was detected in the culture cells.The expression was increased within 8 hours after the injury.Conclusions The expression of EDA-FN would be an ideal marker for estimation of wound healing

Key words: FN, EDA, wound interval estimation, fibroblast

HE MENG;YU SONG;XUE AI-MIN;SHEN YI-WEN;CHEN LONG;GU YUN-JU;ZHAO ZI-QIN(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLAGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA). Expression of EDA-fibronectin in Injured Human Skin Fibroblast and its Potential Application in Estimation of Wound Healing[J]. , 2007, 0(2): 94-96+1.

[1]	Shi-qiu MENG, Jie SHI. Abuse of Pharmaceutical Drugs and Its Prevention [J]. Journal of Forensic Medicine, 2021, 37(6): 788-795.
[2]	Rui BAI, Bing XIE, Bin CONG, Chun-ling MA, Di WEN. Epidemiological Characteristics of Sedative-Hypnotics and Opioid Painkillers at High-Frequency Exposure [J]. Journal of Forensic Medicine, 2021, 37(5): 694-698.
[3]	SHI Yin-tao, WANG Hui-jun, WANG Jun-wei, et al.. Rapid Determination of 13 Sedative Substances and Their Metabolites in Blood by LC-MS/MS [J]. 法医学杂志, 2020, 36(1): 45-51.
[4]	HUANG Jun-jie, YAO Yi, XIA Chong-Jian，et al.. Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age [J]. 法医学杂志, 2019, 35(2): 149-153.
[5]	YU TIAN-SHUI, GUAN DA-WEI, CHANG LIN, ET AL.. Time-dependent Appearances of Myofibroblasts during the Repair of Contused Skeletal Muscle in Rat and Its Application for Wound Age Determination [J]. , 2015, 31(1): 1-6.
[6]	YU TIAN-SHUI, GUAN DA-WEI, ZHAO RUI，等. Correlation between Percentages of PMN, MNC, FBC and Wound Age after Skeletal Muscle Injury in Rats [J]. , 2014, 30(3): 166-168.
[7]	YU TIAN-SHUI, LING YUE, GUAN DA-WEI. Progress in Myofibroblast and Its Application in Forensic Medicine [J]. , 2013, 29(2): 140-143.
[8]	YUAN XIANG-TIAN, PENG XUE, JIN ZHAN-FENG. Expression of FAPα and TGF-β1 in Sudden Deaths due to Acute Myocardial Ischemia [J]. , 2012, 28(1): 18-20.
[9]	FAN YAN-YAN, GUAN DA-WEI, WANG TAO, ET AL. Time-dependent Recruitment and Differentiation of Fibrocytes in Mouse Skin Wound Healing [J]. , 2011, 27(4): 246-249.
[10]	GAO YANG;PENG XUE;JIN ZHAN-FEN;FU ZHI-JUN (. DEPARTMENT OF FORENSIC PATHOLOGY;HARBIN MEDICAL UNIVERSITY;HARBIN 00;CHINA;. DEPARTMENT OF FORENSIC MEDICINE;HARBIN MEDICAL UNIVERSITY;HARBIN 00;CHINA). Expression of FAP and α-SMA During the Incised Wound Healing in Mice Skin [J]. , 2009, 0(6): 405-408.
[11]	HU LING;CHEN RUI;YU BIN-HUA;CHEN XIAO-GANG;LIU MIN(.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.GUANGDONG NANTIAN FORENSIC JUDICAL APPRAISAL INSTITUTE;SHENZHEN 0;CHINA;.DEPARTMENT OF FOR. In Vitro Study on bFGF Expression of Rat Astrocytes after Mechanical Injury [J]. , 2009, 0(5): 326-328+.
[12]	CHEN RUI;YU BIN-HUA;HU LING;YAN ZHI;WANG WEN-DONG;LIU MIN (. WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;. DEPARTMENT OF FORENSIC MEDICINE;GUANGDONG MEDICAL COLLEGE;DONGGUAN 0;. Changes of HSP70, bFGF and TGF-β1 Expression in Rat Brain after Concussion [J]. , 2009, 0(4): 249-253.
[13]	YU SONG;XUE AI-MIN;HE MENG;ZHAO ZI-QIN;SHEN YI-WEN;GU YUN-JU(.HUZHOU PUBLIC SECURITY BUREAU;HUZHOU 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA). The Relationship Between Fibronectin EDA/EDB mRNA in Skin Fibroblast and Injury Time [J]. , 2007, 0(3): 188-190.
[14]	LIU NING-GUO;CHEN YI-JIU;LU REN-QUAN;HUANG XIAO-HUA;ZHANG JIAN-HUA (. INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;CHINA;.SHANGHAI METERIA MEDICA BIOENGIERING INSTITUTE;SHANGHAI 0000;CHINA). Preliminary Application on Test Paper of Gold-labelled Antibody against Fibronectin EⅢA Splicing Variant [J]. , 2006, 0(2): 81-83.
[15]	HUANG DAI-XIN;ZHANG LIN;WU MEI-YUN;ET AL. (.FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;. COLLEGE OF ELEMENTARY MEDICINE AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENG. The expression of bFGF and its receptor FGFR1 in rat after fluid-percussive brain injury [J]. , 2004, 0(2): 65-67+1.

Expression of EDA-fibronectin in Injured Human Skin Fibroblast and its Potential Application in Estimation of Wound Healing

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