Detection of MMP-11 from Menstrual Blood Using Immunohistochemistry

Abstract

Abstract: Objective To prove the feasibility of detecting menstrual blood as well as its cellular localization with rabbit-anti-human matrix metalloproteinase-11(MMP-11) polyclonal antibody. Methods MMP-11 in menstrual blood, peripheral blood, vaginal liquid, aged menstrual bloodstain, and endometrium sections were assayed with SAP immunohistochemistry. Results MMP-11 was found only in menstrual samples within stroma and epithelium cells. Conclusion MMP-11 polyclonal antibody may be applied in the distinction between menstrual blood and venous blood.

Key words: immunohistochemistry, polyclonal antibodies, MMP-11, menstrual blood

YAO YA-NAN;LU HUI-LING;CHEN SEN;ZHENG JING;ZHANG YA-QING (.DEPARTMENT OF FORENSIC BIOLOGY;ZHONGSHAN SCHOOL OF MEDICINE;SUN YAT-SEN UNIVERSITY;GUANGZHOU 000;CHINA;.THE FIRST AFFILIATED HOSPITAL OF SUN YAT-SEN UNIVERSITY;GUANGZHOU;000;. Detection of MMP-11 from Menstrual Blood Using Immunohistochemistry[J]. , 2008, 0(1): 32-33+8.

[1]	Qi ZHANG, He-miao ZHAO, Kang YANG, Jing CHEN, Rui-qin YANG, Chong WANG. Construction of an Analysis Model of mRNA Markers in Menstrual Blood Based on Naïve Bayes and Multivariate Logistic Regression Methods [J]. Journal of Forensic Medicine, 2023, 39(5): 447-451.
[2]	Xue-shi CHEN, Jun CHU, Li-jun YANG, Tao WANG, Lu-yang TAO. Application of Immunohistochemistry and Special Staining Technique in Forensic Traumatic Pathology Identification [J]. Journal of Forensic Medicine, 2021, 37(5): 666-672.
[3]	WU Mao-wang, SU Li, HE Fang, et al. Differential Expression of Integrin β1 in Two Brain Injury Models of Rats [J]. 法医学杂志, 2020, 36(4): 502-506.
[4]	HE Hong-xia, JI An-quan, HAN Na, et al. Identification of Peripheral Blood and Menstrual Blood Based on the Expression Level of MicroRNAs and Discriminant Analysis [J]. 法医学杂志, 2020, 36(4): 514-518，524.
[5]	CHEN Jing-wei, WANG Peng-fei, ZHANG Meng-zhou1，et al.. Relationship between Expression Changes of CB2R and Wound Age of Brain Contusion in Mice [J]. 法医学杂志, 2019, 35(2): 136-142.
[6]	ZHAO Jian-xin, JIN Xin, HUANG Jun-jie，et al.. Time-dependent Expression and Distribution of AChE during the Skin Incised Wound Healing in Mice [J]. 法医学杂志, 2019, 35(2): 143-148.
[7]	WANG YING-XI, ZHU XIAO-JUN, JIAO ZHANG-PING, ET AL.. A New Method of Identifying the Peripheral Blood and the Menstrual Blood [J]. , 2012, 28(5): 359-361.
[8]	WEI SHU-RONG, CHEN XIN-SHAN, CHEN HUANG-FENG, ET AL.. Expression of Fas Protein of Myocardium in Dilated Cardiomyopathy [J]. , 2012, 28(4): 252-255.
[9]	ZHANG YA-QING, LU HUI-LING, YAO YA-NAN. Detection of Matrix Metalloproteinase-11 in Menstrual Blood by Enhanced Chemiluminescence Method [J]. , 2012, 28(2): 109-111.
[10]	YUAN XIANG-TIAN, PENG XUE, JIN ZHAN-FENG. Expression of FAPα and TGF-β1 in Sudden Deaths due to Acute Myocardial Ischemia [J]. , 2012, 28(1): 18-20.
[11]	WANG FU-YUAN, LI RU-BO, LIANG HONG-XIA, ET AL. Homer Protein Expression after Focal Brain Contusion in Rats [J]. , 2011, 27(6): 409-412.
[12]	PENG XUE, JIN ZHAN-FENG, SHAO HONG-JIANG, ET AL. Immunohistochemical Expression of HO-1 in Traumatic Human Brain Tissue [J]. , 2010, 26(5): 325-327,331.
[13]	ZHU JIAN-HUA, HU YONG-LIANG, ZHANG WU, ET AL. Effects of Triptolide on Hypothalamic-pituitary-adrenal Axis of Rats [J]. , 2010, 26(4): 260-265.
[14]	GUO XIAO-CHONG;LI RU-BO;LIANG HONG-XIA;WANG FU-YUAN;WU XU (. DEPARTMENT OF FORENSIC PATHOLOGY;SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA;. KEY LAB OF LABORATORY TRANSGENIC ANIMAL OF LIAONING PROVINCE;DEPA. Expression of Matrix Metalloproteinase-3 after Brain Contusion in Rats [J]. , 2009, 0(1): 1-5.
[15]	CHEN ZONG-YUN;WANG JING-YU;LI YONG-HONG (DEPARTMENT OF FORENSIC MEDICINE;WANNAN MEDICAL COLLEGE;WUHU 000;CHINA). The Expression of NGF after Rat Diffuse Axonal Injury [J]. , 2008, 0(3): 172-174.

Detection of MMP-11 from Menstrual Blood Using Immunohistochemistry

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