Journal of Forensic Medicine ›› 2025, Vol. 41 ›› Issue (2): 197-200.DOI: 10.12116/j.issn.1004-5619.2024.340404
Special Issue: 中毒的法医学鉴定专题
• Topic on Forensic Identification of Poisoning • Previous Articles
Received:2024-04-10
Online:2025-08-11
Published:2025-04-25
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URL: http://www.fyxzz.cn/EN/10.12116/j.issn.1004-5619.2024.340404
| 基因 | SNP位点 | 引物序列（5´→3´） | 基因变异后果 | 本例变异情况 |
|---|---|---|---|---|
| CYP3A4 | （rs2242480，G>A） CYP3A4*1G | F：CACCCTGATGTCCAGCAGAAACT； R：AATAGAAAGCAGATGAACCAGAGCC | 该变异是增加美沙酮致死风险可能性的重要变异[ | GA突变杂合子 |
| CYP2B6 | （rs3745274，G>T） CYP2B6*9 | F：GTCTGCCCATCTATAAAC； R：CTGATTCTTCACATGTCTGCG | 该变异等位基因携带者比非携带者需要更低的美沙酮治疗剂量[ | GG野生纯合子 |
| CYP2C19 | （rs4986893，G>A） CYP2C19*3 | F：ACCCTGTGATCCCACTTTCATC； R：TCAGGAAGCAAAAAACTTGGCC | 缺乏功能性等位基因的变异基因型导致（R）-美沙酮的代谢降低，从而提高美沙酮血浆水平[ | GA突变杂合子 |
Tab. 1 PCR primer sequences， variant consequences and variations of the case forCYP3A4， CYP2B6 and CYP2C19 SNP loci
| 基因 | SNP位点 | 引物序列（5´→3´） | 基因变异后果 | 本例变异情况 |
|---|---|---|---|---|
| CYP3A4 | （rs2242480，G>A） CYP3A4*1G | F：CACCCTGATGTCCAGCAGAAACT； R：AATAGAAAGCAGATGAACCAGAGCC | 该变异是增加美沙酮致死风险可能性的重要变异[ | GA突变杂合子 |
| CYP2B6 | （rs3745274，G>T） CYP2B6*9 | F：GTCTGCCCATCTATAAAC； R：CTGATTCTTCACATGTCTGCG | 该变异等位基因携带者比非携带者需要更低的美沙酮治疗剂量[ | GG野生纯合子 |
| CYP2C19 | （rs4986893，G>A） CYP2C19*3 | F：ACCCTGTGATCCCACTTTCATC； R：TCAGGAAGCAAAAAACTTGGCC | 缺乏功能性等位基因的变异基因型导致（R）-美沙酮的代谢降低，从而提高美沙酮血浆水平[ | GA突变杂合子 |
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