法医学杂志

法医学杂志 ›› 2021, Vol. 37 ›› Issue (4): 486-492.DOI: 10.12116/j.issn.1004-5619.2021.310304

所属专题： 新精神活性物质

• 专题 • 上一篇    下一篇

可疑植物制品中合成大麻素5F-EDMB-PICA的鉴定

孟鑫1， 花镇东1， 贾薇1， 刘翠梅1， 刘杉2， 陈月猛2   

  1. 1. 公安部禁毒情报技术中心 毒品监测管控与禁毒关键技术公安部重点实验室，北京 100193； 2. 贵阳市公安局禁毒支队，贵州 贵阳 550081
  • 发布日期:2021-08-25 出版日期:2021-08-28
  • 通讯作者: 刘翠梅，女，博士，副研究员，主要从事毒品鉴定与特征分析研究；E-mail：liucuimei8258@163.com 陈月猛，男，工程师，主要从事毒品检验鉴定；E-mail：cym13984091203@163.com
  • 作者简介:孟鑫（1982—），男，博士，主要从事毒品鉴定与特征分析研究；E-mail：mengxin_nnl@163.com
  • 基金资助:
    国家重点研发计划资助项目（2016YFC0800903）

Identification of Synthetic Cannabinoid 5F-EDMB-PICA in Suspicious Herbal Products

MENG Xin1, HUA Zhen-dong1, JIA Wei1, LIU Cui-mei1, LIU Shan2, CHEN Yue-meng2   

  1. 1. Key Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security, Beijing 100193, China; 2. Drug Control Detachment of Guiyang Public Security Bureau, Guiyang 550081, China
  • Online:2021-08-25 Published:2021-08-28

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摘要： 目的 探讨无对照品时可疑植物制品中未知合成大麻素的定性分析策略。 方法 采用甲醇对植物制品中的合成大麻素进行提取，提取液经旋转蒸发仪浓缩后采用制备液相分离、纯化，得到高纯度合成大麻素制备样品，综合利用气相色谱-质谱法（gas chromatography-mass spectrometry，GC-MS）、超高效液相色谱-四极杆飞行时间质谱（ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry，UPLC-QTOF-MS）、核磁共振（nuclear magnetic resonance，NMR）对制备化合物进行结构解析。 结果 采用制备液相得到10 mg高纯度未知样品，采用GC-MS、UPLC-QTOF-MS和NMR进行分析，通过谱图解析，最终确定未知合成大麻素为2-[1-（5-氟戊基）-1H-吲哚-3-甲酰氨基]-3，3-二甲基丁酸乙酯，简称5F-EDMB-PICA。 结论 本研究建立了采用制备液相从低含量植物制品中提取未知合成大麻素的方法，并综合利用GC-MS、UPLC-QTOF-MS、NMR实现了对未知物结构的解析，这些信息将有助于法庭科学实验室在鉴定实践中鉴定该物质或其他具有类似结构的化合物。

关键词: 法医学；毒物分析；新精神活性物质；合成大麻素类；气相色谱-质谱法；超高效液相色谱-四极杆飞行时间质谱；核磁共振；2-[1-（5-氟戊基）-1H-吲哚-3-甲酰氨基]-3, 3-二甲基丁酸乙酯；植物样品

Abstract: Objective To study the qualitative analysis strategy for unknown synthetic cannabinoid in the suspicious herbal product when no reference substance is available. Methods The synthetic cannabinoid in herbal blend was extracted with methanol. The extract was concentrated by rotary evaporator and separated and purified by preparative liquid chromatography, to obtain high purity synthetic cannabinoid sample. Gas chromatography-mass spectrometry （GC-MS）, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-QTOF-MS） and nuclear magnetic resonance （NMR） were used to determine the structure of the prepared compound. Results High purity unknown sample （10 mg） was obtained by preparative liquid chromatography. The sample was analyzed by GC-MS, UPLC-TOF-MS and NMR, and through spectrum analysis, the unknown synthetic cannabinoid was determined as 5F-EDMB-PICA. Conclusion The method to extract unknown synthetic cannabinoid from low content herbal products by preparative liquid chromatography was established, and the structure of the unknown sample was identified by comprehensive use of GC-MS, UPLC-QTOF-MS and NMR. The information will assist forensic laboratories in identifying this substance or other compounds with similar structures in their casework.

Key words: forensic medicine, toxicological analysis, new psychoactive substance, synthetic cannabinoids, gas chromatography-mass spectrometry （GC-MS）, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-QTOF-MS）, nuclear magnetic resonance （NMR）, 5F-EDMB-PICA, plant samples

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