法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (1): 1-5,11.DOI: 10.3969/j.issn.1004-5619.2012.01.001

• 论著 •    下一篇

大鼠骨骼肌挫伤后外周血源性纤维细胞的数量变化

李力强1，于天水1，官大威1，范琰琰1，赵  锐1，马文翔1，张书韬1，2，张  舒1，任  鹏1，李骄勇1，3   

  1. （1. 中国医科大学法医学院法医病理学教研室，辽宁 沈阳 110001； 2. 黑龙江省公安厅，黑龙江 哈尔滨 150008； 3. 伊春市公安局，黑龙江 伊春 153000）
  • 发布日期:2012-02-25 出版日期:2012-02-28
  • 通讯作者: 官大威，男，博士，教授，博士研究生导师，主要从事皮肤损伤修复机制及钝力性心脏外伤研究；E-mail：dwguan@ mail.cmu.edu.cn
  • 作者简介:李力强（1984—），男，辽宁本溪人，硕士研究生，主要从事骨骼肌损伤修复机制的研究
  • 基金资助:

    教育部高等学校博士学科点专项科研基金项目（200801590020）

The Number of Circulating Fibrocytes of Skeletal Muscle in Rats after Contusion

LI LI-QIANG1, YU TIAN-SHUI1, GUAN DA-WEI1, FAN YAN-YAN1, ZHAO RUI1, MA WEN-XIANG1, ZHANG SHU-TAO1,2, ZHANG SHU1, REN PENG1, LI JIAO-YONG1,3   

  1. （1. Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China; 2. Heilongjiang Provincial Public Security Department, Harbin 150008, China; 3. Yichun Public Security Bureau, Yichun 153000, China）
  • Online:2012-02-25 Published:2012-02-28

被引次数

2

摘要： 目的 探讨大鼠骨骼肌挫伤后损伤区外周血来源的纤维细胞数量变化规律。 方法 制作大鼠骨骼肌挫伤模型，应用CD45、procollagenⅠ双重免疫荧光染色法检测大鼠骨骼肌挫伤后各时间点外周血来源的纤维细胞的变化。 结果 对照组大鼠骨骼肌中未见CD45、procollagenⅠ阳性的纤维细胞分布；损伤组大鼠骨骼肌挫伤后6 h～1 d，仅见少数CD45阳性的单个核细胞；伤后3 d，开始有少量CD45、procollagenⅠ阳性的纤维细胞聚集于损伤区；伤后5 d，阳性的纤维细胞数量明显增加；伤后7 d达高峰，此后逐渐减少，同时还发现相对于3 d组，纤维细胞的体积随着时间延长逐渐变大；伤后14 d，纤维细胞胞质中的CD45和procollagenⅠ的表达强度都相应减弱。 结论 大鼠骨骼肌挫伤后，挫伤区外周血来源的纤维细胞的数量变化具有一定的时间规律性，可望作为推断骨骼肌挫伤时间的指标之一。

关键词: 法医病理学;创伤和损伤;肌, 骨骼;纤维细胞;损伤时间;大鼠

Abstract: Objection To investigate the time-dependent appearance of circulating fibrocytes of skeletal muscle in rats after contusion. Methods The model of skeletal muscle wound was established in rat. The circulating fibrocytes in contused skeletal muscle were detected by CD45 and procollagen Ⅰ double immunofluorescence staining method. Results In the control group, CD45- and procollagen Ⅰ-positive cells were not detected in skeletal muscle. A few CD45 cells were observed aged from 6 h to 1 d after contusion. A few CD45- and procollagen Ⅰ-positive cells（fibrocytes） initially gathered in injury area 3 d after injury. The ratio of positive fibrocytes significantly increased 5 d after injury. The ratio of fibrocytes was highest at 7 d after contusion and then decreased. The volume of fibrocytes showed bigger with injury time increase compared with 3 d group. The expression of procollagen Ⅰ and CD45 were weakened at 14 d after injury. Conclusion The circulating fibrocytes are detected in contused skeletal muscle in time-dependent pattern. Circulating fibrocytes may be a marker in the wound age determination for contused skeletal muscle.

Key words: forensic pathology, wounds and injuries, muscle, skeletal, fibrocyte, wound age, rats

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