法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (3): 177-180.DOI: 10.3969/j.issn.1004-5619.2015.03.001

• 论著 •    下一篇

大鼠肌肉挫伤后COX6C mRNA的表达与损伤时间关系

孙俊红1，张  镭1，2，王小伟1，2，杜秋香1，路  健1，王英元1   

  1. （1. 山西医科大学法医学院，山西 太原 030001； 2. 重庆市公安局刑警总队，重庆 400021）
  • 发布日期:2015-06-25 出版日期:2015-06-28
  • 通讯作者: 王英元，男，教授，博士研究生导师，主要从事法医病理学（损伤及死亡时间）研究；E-mail：wyy580218@163.com
  • 作者简介:孙俊红（1975—），男，山西稷山人，博士研究生，主要从事法医损伤病理学研究；E-mail：junhong.sun@sxmu.edu.cn
  • 基金资助:

    国家自然基金委青年基金资助项目（81001347）

Relation between Injury Time and the Expression of COX6C mRNA in Skeletal Muscle of Rats after Contusion

SUN JUN-HONG1, ZHANG LEI1,2, WANG XIAO-WEI1,2, DU QIU-XIANG1, LU JIAN1, WANG YING-YUAN1   

  1. （1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Criminal Police Department of Chongqing Public Security Bureau, Chongqing 400021, China）
  • Online:2015-06-25 Published:2015-06-28

被引次数

1

摘要： 目的 探讨肌肉挫伤后组织中细胞色素c氧化酶亚基Ⅵc（cytochrome c oxidase subunit Ⅵc，COX6C） mRNA的表达变化及其与损伤时间的关系。 方法 54只健康成年SD大鼠随机被分为正常对照组和损伤后0.5、1、6、12、18、24、30和36 h组，采用重力锤自由落体方式制作大鼠肌肉挫伤模型。于相应时间提取挫伤处肌肉组织样本进行常规组织学观察，并提取组织中总RNA采用real-time PCR检测COX6C mRNA相对表达量。 结果 损伤后6 h内仅见肌纤维间出血、肌细胞肿胀等改变，未见炎症细胞浸润。损伤6 h以后逐渐出现肌细胞变性、坏死、炎症细胞浸润、纤维结缔组织增生等改变。COX6C mRNA在损伤后6 h以前其表达量均高于正常对照组，而在损伤6~36 h，其表达量均低于正常对照组。 结论 COX6C mRNA在大鼠肌肉挫伤后呈现规律性表达，结合组织学检查有助于损伤时间的推断和研究。

关键词: 法医病理学, 创伤和损伤, 肌肉, 细胞色素c氧化酶Ⅵc亚基, 大鼠

Abstract: Objective To investigate the relation between injury time and the expression of cytochrome c oxidase subunit Ⅵc （COX6C） mRNA in skeletal muscle of rat after contusion. Methods A total of fifty-four SD rats were divided into the control group and the contusion groups （0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion）, randomly. The contusion model was established by free fall drop of gravity hammer. At corresponding time point after contusion, the regular histology was examined and expression level of COX6C mRNA was tested by real-time PCR after extraction of total RNA from the tissues. Results The main pathological features of 6 h after injury included edema and hemorrhage in myocytes with no inflammatory cells found. After 6 hours, the findings included myocyte degeneration and necrosis, inflammatory cells infiltration, and fibrous connective tissue proliferation in the contused zone. The expression level of COX6C mRNA was higher than that of the control group within 6 h after contusion. The expression level was lower than that of the control group from 6-36 h after contusion. Conclusion The level of COX6C mRNA expresses in a regular way after contusion. It may be useful for estimating wound age in combination with the results of pathological features.

Key words: forensic pathology, wounds and injuries, muscle, cytochrome c oxidase subunit Ⅵc, rats

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