法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (5): 325-328.DOI: 10.3969/j.issn.1004-5619.2014.05.002

• 论著 • 上一篇    下一篇

急性乙醇中毒和外伤在蛛网膜下腔出血致死机制中的作用

汤艺坤1，2，史  萌1，欧桂生3，赵  虎1   

  1. （1. 中山大学中山医学院法医学系，广东 广州 510080； 2. 广州市公安局越秀区分局，广东 广州 510080； 3. 广东省公安厅刑事技术中心，广东 广州 510050）
  • 发布日期:2014-10-25 出版日期:2014-10-28
  • 通讯作者: 赵虎，男，教授，主要从事法医学教学、科研和检案工作；E-mail：hzhao@163.com
  • 作者简介:汤艺坤（1977—），男，福建云霄人，主检法医师，主要从事法医学检案和科研工作；E-mail：2810872190@qq.com

Role of Acute Alcohol Poisoning and Craniocerebral Trauma in the Mechanism of Death Caused by Subarachnoid Hemorrhage

TANG YI-KUN1,2, SHI MENG1, OU GUI-SHENG3, ZHAO HU1   

  1. （1. Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; 2. Yuexiu Branch of Guangzhou Public Security Bureau, Guangzhou 510080, China; 3. Forensic Science Center of Guangdong Provincal Public Security Bureau, Guangzhou 510050, China）
    Abstract：
  • Online:2014-10-25 Published:2014-10-28

被引次数

5

摘要： 目的 研究人脑在急性乙醇中毒状态下tPA、MMP-2、MMP-9及AEG-1表达水平与乙醇浓度的关系，分析乙醇与外伤在蛛网膜下腔出血死亡机制中的作用。 方法 选取15例实际案例，提取脑组织进行组织病理学检查、心血进行乙醇浓度检测，用免疫组织化学法观察人脑干、大脑、小脑tPA、MMP-2、MMP-9及AEG-1表达水平。 结果 急性乙醇中毒外伤组和急性乙醇中毒组的脑组织肿胀。除小脑tPA外，tPA、MMP-2、MMP-9及AEG-1表达水平在饮酒者较未饮酒者在脑干、大脑、小脑均有升高（P<0.05），MMP-2、MMP-9和AEG-1表达水平与心血乙醇浓度存在正相关（P<0.05，r>0.6）。 结论 急性乙醇中毒状态下人脑tPA、MMP-2、MMP-9、AEG-1表达较未饮酒者均显著增强，MMP-2、MMP-9及AEG-1表达水平与乙醇浓度存在相关性，乙醇对脑细胞有毒性作用。

关键词: 法医病理学, 创伤和损伤, 蛛网膜下腔出血, 乙醇

Abstract: Objective To investigate the relation between the expression of tPA, MMP-2, MMP-9 and AEG-1 in the human brain tissue and the ethanol concentration under the acute alcohol poison, and to analyze the role of alcohol and trauma in the mechanism of death of subarachnoid hemorrhage. Methods Fifteen real cases were collected in this study. The brain tissues were researched by histological examination and the concentration of ethanol in heart blood were detected. The tPA, MMP-2, MMP-9 and AEG-1 in brainstem, brain and cerebellum were observed respectively by immunohistochemistry. Results In alcohol poisoning groups with or without trauma, the acute alcohol toxicity resulted in the swelling of brain tissues. The tPA, MMP-2, MMP-9 and AEG-1 of brainstem, brain and cerebellum showed high expression in alcohol victims, and the tPA in cerebellum showed no difference. The expression of the MMP-2, MMP-9 and AEG-1 showed good relation with the ethanol concentration in blood （P<0.05, r>0.6）. Conclusion The expressions of tPA, MMP-2, MMP-9 and AEG-1 are significant higher in alcohol victims, and expressions of MMP-2 and MMP-9 and AEG-1 have positive correlation with the alcohol concentration. The alcohol has acute toxicity to brain cells.

Key words: forensic pathology, wounds and injuries, subarachnoid hemorrhage, ethanol

中图分类号: