法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (2): 138-141.DOI: 10.3969/j.issn.1004-5619.2018.02.006

• 论著 • 上一篇    下一篇

植物类物证DNA遗传标记鉴定系统的建立

李  辉1，夏  攀1，王  创2，汪  远3，赵雪莹1，马  克1，戴  维1，曹  禹1，周怀谷1，刘文斌1   

  1. 1. 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室 上海市刑事科学技术研究院，上海 200083； 2. 浙江大学植物生理学及生物化学国家重点实验室，浙江 杭州 310058； 3. 中国科学院上海辰山植物科学研究中心，上海 201602
  • 发布日期:2018-04-25 出版日期:2018-04-28
  • 通讯作者: 刘文斌，男，副研究员，主要从事刑事科学技术研究；E-mail：wbliu1981@163.com
  • 作者简介:李辉（1986—），男，硕士，助理研究员，主要从事法医物证学研究；E-mail：lihui860225@163.com
  • 基金资助:
    公安部科技强警基础工作专项资助项目（2014GABJC 008）；上海市公安局科学技术发展基金资助项目（2016019）

Establishment of DNA Genetic Marker Identification System for Plant Evidence

LI Hui1, XIA Pan1, WANG Chuang2, WANG Yuan3, ZHAO Xue-ying1, MA Ke1, DAI Wei1, CAO Yu1, ZHOU Huai-gu1, LIU Wen-bin1   

  1. 1. Key Laboratory of Forensic Evidence and Science Technology of Ministry of Public Security, Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Research Institute of Criminal Science and Technology, Shanghai 200083, China; 2. State Key Laboratory of Plant Physiology and Biochemistry, Zhejiang University, Hangzhou 310058, China; 3. Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China
  • Online:2018-04-25 Published:2018-04-28

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摘要： 目的 建立一套以DNA遗传标记为基础的植物类物证检材的种属鉴定系统。 方法 收集上海地区常见植物200例，从形态学上确定物种，设计扩增rbcL、matK、ITS基因片段的引物，用琼脂糖凝胶电泳检测PCR产物并测序，评估三个标记的通用性和识别能力。 结果 在检测成功率方面，rbcL（99.5%）>matK（92.5%）>ITS（86.0%）；rbcL+matK组合检测优于单rbcL或单matK的识别能力，其识别能力主要在属及属以上水平；ITS检测结果不稳定，不适合作为单标记，但可作为有力补充，rbcL+matK+ITS组合的识别能力明显高于rbcL+matK组合，对大部分植物样本种属鉴定能够达到属及属以下水平。 结论 以rbcL+matK+ITS组合作为标记的鉴定系统对植物类物证有良好的通用性，其识别能力对大部分植物样本能够达到属及属以下水平。

关键词: 法医遗传学；DNA, 植物；遗传标记；种属鉴定

Abstract: Objective To establish a species identification system based on DNA genetic markers for plant evidence. Methods Two hundred common plants in Shanghai were collected and identified by morphological characteristics. The primers of gene segments rbcL, matK, and ITS were designed and amplified. The PCR amplicon was detected by agarose gel electrophoresis. After the sequencing, the universality and the identification capacity of the three markers were evaluated. Results The success rate of amplification was in order of rbcL （99.5%） > matK （92.5%） > ITS （86.0%）. The identification capacity of the combination of rbcL and matK was better than that of rbcL or matK, by which most plant species could be identified to the genus or higher. ITS was not suitable to be a unique marker because of its unstable result, but it still could be a powerful supplement. The identification capacity of the combination of rbcL, matK and ITS was higher than that of rbcL and matK, by which most plant species could be identified to the genus or lower. Conclusion The identification system with the combination of rbcL, matK and ITS as markers has excellent universality for plant evidence, which can distinguish most plant species to the genus or lower.

Key words: forensic genetic, DNA, plant, genetic markers, species identification