法医学杂志

法医学杂志 ›› 2021, Vol. 37 ›› Issue (2): 220-224.DOI: 10.12116/j.issn.1004-5619.2020.500604

• 技术与应用 • 上一篇    下一篇

全自动核酸提取仪联合真空浓缩仪在法医学中的应用

李淑侠1， 沈金建1， 曹佩军1， 李志超2   

  1. 1. 平湖市公安局，浙江 平湖 314200； 2. 上海市公安局奉贤分局，上海 201499
  • 收稿日期:2020-06-16 发布日期:2021-04-25 出版日期:2021-04-28
  • 作者简介:李淑侠（1978—），女，主检法医师，主要从事法医物证学检验；E-mail：745202774@qq.com

Application of Automatic Nucleic Acid Extractor Combined with Vacuum Concentrator in Forensic Science

LI Shu-xia1, SHEN Jin-jian1, CAO Pei-jun1, LI Zhi-chao2   

  1. 1. Pinghu Public Security Bureau, Pinghu 314200, Zhejiang Province, China; 2. Fengxian Branch of Shanghai Public Security Bureau, Shanghai 201499, China
  • Received:2020-06-16 Online:2021-04-25 Published:2021-04-28

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摘要： 目的 探讨全自动核酸提取仪联合真空浓缩仪在法医学DNA提取中的应用价值。 方法 采集人外周静脉血稀释成40、80、120、160、200、240、280和320倍的梯度样本，并对每一梯度的样本分别进行无抑制剂、加黑机油、加铁锈、加果酸、加锡纸、加靛蓝6种处理。应用全自动核酸提取仪对上述样本进行DNA纯化提取，提取后的DNA洗脱液取6 μL直接进行扩增，剩余部分使用真空浓缩仪进行浓缩，浓缩前后的DNA均使用Investigator 26plex QS试剂盒进行扩增、检测。 结果 添加果酸的梯度样本仅在40倍稀释时获得完整STR分型结果，另外5种方式处理的梯度样本在40~160倍稀释时均获得完整的STR分型。DNA浓缩后的STR分型结果显示，平均峰高和基因座检出率均有一定程度的提高，但是效果并不明显。 结论 全自动核酸提取仪具有高效的抑制剂去除能力和DNA提取效率，真空浓缩仪可在一定程度上浓缩DNA，两者联合使用可提高法医学检材的检验成功率。

关键词: 法医遗传学, DNA提取, 微量DNA, STR分型, 抑制剂, 全自动核酸提取仪, 真空浓缩仪

Abstract: Objective To explore the application value of automatic nucleic acid extractor combined with vacuum concentrator in forensic DNA extraction. Methods Gradient samples of human peripheral venous blood were collected at 40, 80, 120, 160, 200, 240, 280 and 320 fold dilution. The samples of each gradient were treated with no inhibitor, black oil, rust, fruit acid, tin foil and indigo, respectively. The automatic nucleic acid extractor was used for DNA purification and extraction of the above samples. The extracted DNA eluent （6 μL） was taken for amplification directly, and the rest was concentrated by vacuum concentrator. DNA was amplified and examined using the Investigator 26plex QS kit before and after concentration. Results Only gradient samples treated with fruit acid obtained complete STR typing results at 40 fold dilution. The other 5 methods obtained complete STR typing results at 40-160 fold dilution. The results of STR typing after DNA concentration showed that the average peak height and detection rates of gene loci both increased to a certain extent, but the effect was not obvious. Conclusion The automatic nucleic acid extractor has an efficient inhibitor removal ability and high extracting efficiency of DNA. The vacuum concentrator can concentrate DNA samples to a certain extent. Combining the automatic nucleic acid extractor with the vacuum concentrator can improve the examination success rate of forensic materials.

Key words:  forensic genetics, DNA extraction, trace DNA, STR typing, inhibitor, automatic nucleic acid extractor, vacuum concentrator

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