法医学杂志

法医学杂志 ›› 1992, Vol. 0 ›› Issue (3): 114-118+.

• 论文 • 上一篇    下一篇

兔急性心肌缺血脱氢酶组织化学和血清酶学变化的综合研究

张国华;李德祥;   

  1. 中国医科大学法医学系,中国医科大学法医学系 沈阳 110001,沈阳 110001
  • 发布日期:1992-06-25 出版日期:1992-06-28

SYNTHETICAL STUDY OF HISTOCHEMICAL AND SERUMAL CHANGES OF DEHYDROGENASES IN RABBIT'S ACUTE MYOCARDIAL ISCHEMIA

ZHANG GUOHUA LI DEXIANG DEPARTMENT OF FORENSIC PATHOLOGY, FACULTY OF FORENSIC MEDICINE, CHINA MEDICAL UNIVERSITY   

  • Online:1992-06-25 Published:1992-06-28

被引次数

3

摘要： 为了深入研究急性心肌缺血的脱氢酶变化,作者通过结扎兔冠状动脉左前降枝的动物模型进行了实验,观察了结扎后0.25,0.5、0.1、1、1.5、2、4、6、12、24、36、72小时的脱氢酶变化。结果表明;1)急性心肌缺血6小时血清乳酸脱氢酶活性显著升高,其缺血前后差别是734±153.66nmol/L(差数平均值±标准差,P<0.05);2)结扎冠状动脉0.5小时后,大体标本组织化学染色示心肌梗塞;3)结扎冠状动脉1小时后微观酶组织化学示LDH和MDH活性下降,SDH在冠状动脉被扎2小时后亦呈同样变化。LDH及MDH在心肌缺血时的改变比SDH敏感;4)心肌缺血时其局部酶组织化学早于血清酶改变。

关键词: 急性心肌缺血, 琥珀酸脱氢酶(SDH), 乳酸脱氢酶(LDH), 苹果酸脱氢酶(MDH), 组织化学, 血清乳酸脱氢酶

Abstract: In order to further study the changes of dehydrogenases in acute myocardial ischemia (AMI), the present experiments were performed in a rabbit model of ligating left anterior descending coronary artery for 0.25, 0.5, 1, 1.5, 2, 4, 6, 12, 24, 36 and 72 hours. Tde rusults showed: (1) By 6 hours of AMI, the activity of seminal lactate dehydrogenase (LDH) was obviously high, its difference between pre-ischemia and post-ischemia was 734±153.66nmol/L (Mean±SE, P<0.05, t test). (2) At 0.5 hours after coronary ligation, the myocardial infarction was identified by macroscopic histochemistry. (3) At 1 hour after coronary ligation, LDH and malate dehydrogenase (MDH) depleted, so did succinate dehydrogenase(SDH) at 2 hours after ligation By microscopic histochemistry. LDH and MDH were more sensitive than SDH in AMI. (4) The changes of local enzymatic histochemistry of ischemic myocardium occured prior to the total serumal enzymatic ones.