法医学杂志

法医学杂志 ›› 1999, Vol. 15 ›› Issue (3): 144-146+.

• 论文 • 上一篇    下一篇

用多重PCR及荧光检测技术分析CTT位点的多态性

阙庭志;李莉;林源;吴福根;叶元康;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,上海市铁道医学院附属甘泉医院 上海 200063,上海 200063,上海 200063,上海 200063,上海 200065
  • 发布日期:1999-06-25 出版日期:1999-06-28

Population study of the CSF1PO, TPOX and TH01 STR polymorphisms in east China

QUE TINGZHI, LI LI, LIN YUAN, ET AT (INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, SHANGHAI 200063, P. R. CHINA)   

  • Online:1999-06-25 Published:1999-06-28

被引次数

9

摘要： 运用PCR和荧光检测技术对中国华东地区98份无血缘关系的个体血样进行了3个STR位点的复合分型检验。CSF1PO位点检测到9个等位基因、22种基因型;TPOX位点检测到8个等位基因、22种基因型;TH01位点检测到7个等位基因、14种基因型。经计算,得到华东地区CSF1PO、TPOX和TH01位点等位基因频率分布值和杂合度H、鉴别能力(DP)、排除概率(PE)、多态信息量(PIC)及3个位点的累积识别能力等数据资料。

关键词: DNA检验, PCR, 群体遗传学, STR, CSF1PO, TPOX, TH01

Abstract: Allele and genotype frequencies of four short tandem repeat loci were determined in a population sample from east China by using PCR and fluorescence techniques. After denaturing PAGE, 9 alleles were identified for CSF1PO, 8 alleles for TPOX, and 7 alleles for TH01. All loci met Hardy-Weinberg expectations. In addition, the theoretical values of these loci for paternity and forensic casework were evaluated.

Key words: forensic science, DNA typing, PCR, population genetics, STR, CSF1PO, TPOX, TH01