法医学杂志

法医学杂志 ›› 2000, Vol. 0 ›› Issue (1): 6-7+63.

• 论文 • 上一篇    下一篇

VEGF免疫组化染色在冠心病猝死诊断中的应用

陈建国,徐小虎,姚青松,汪冠三,祝家镇   

  1. 中山医科大学法医病理学教研室!广东广州510089,汕头大学医学院病理学教研室!广东汕头515031,广州市公安局刑科所!广东510030,广州市公安局刑科所!广东510030,中山医科大学法医病理学教研室!广东广州510089
  • 发布日期:2000-02-25 出版日期:2000-02-28

Immunohistochemical studies on vascular endothelial growth factor (VEGF) for the postmortem diagnosis of sudden coronary death

CHEN JIANGUO, ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY,SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES,GUANGZHOU 510089,P.R.CHINA   

  • Online:2000-02-25 Published:2000-02-28

被引次数

12

摘要： 运用免疫组化SABC法和图像分析与统计学处理系统 ,对16例冠心病猝死和15例非冠心病猝死对照组尸检心脏标本心肌局部血管内皮生长因子 (VEGF)的表达进行了研究。结果 :16例冠心病猝心脏标本心肌梗死局部心肌均有VEGF的阳性表达 ,尤以心肌梗死灶边缘为甚 ,阳性表达率为100 %。15例非冠心病猝死对照组心脏标本仅有2例心肌局部散在有VEGF弱阳性表达 ,其余为阴性。将免疫组化染色结果进行图像定量分析与统计学处理 ,冠心病猝死组阳性指数为13.68±2.73 ,对照组为2.05±0.84 ,两组结果有极显著性差异 (P<0.01)。本研究结果提示 ,VEGF免疫组化染色可望为冠心病猝死的死后诊断提供一个比较客观的病理形态学依据

关键词: 冠心病猝死, 血管内皮生长因子, 免疫组织化学

Abstract: The expression of VEGF in myocardium of the heart of sudden coronary death (SCD; n=16) and of the control group (n=15) was studied quantitatively by immunohistochemistry SABC method, image analysis and statistical process. The results showed that local infarcted myocardium of the heart from all SCD had strong positive expression of VEGF, especially in peri-infarction area. The rate of positive expression was 100%, while only two of the 15 in control group had mild positive expression sparsely in local myocardium. Image analysis and statistical process showed that the positive index in SCD group was 13.68±2.73, and in the control group 2.05±0.84. There was a significant difference between the two groups (P< 0.01). The results suggested that the immunohistochemical staining of VEGF with image analysis and statistical process be used as an objective marker for the postmortem diagnosis of SCD.

Key words: sudden coronary death, vascular endothelial growth factor, Immunohistochemistry