法医学杂志

法医学杂志 ›› 2000, Vol. 0 ›› Issue (3): 137-138+.

• 论文 • 上一篇    下一篇

人脑挫伤后GFAP、PCNA的法医病理学研究

陶陆阳,汪德文,刘萍,喻少波,郝春雨,吴旭   

  1. 中国医科大学!辽宁沈阳110001,中国医科大学!辽宁沈阳110001,辽宁中医学院!辽宁沈阳110031,沈阳刑事科学技术研究所!辽宁110002,沈阳刑事科学技术研究所!辽宁110002,中国医科大学!辽宁沈阳110001
  • 发布日期:2000-06-25 出版日期:2000-06-28

The changes of GFAP and PCNA after human brain contusion: an immunohistochemical study in forensic pathology

TAO LUYANG, ET AL ( INSTITUTE OF FORENSIC SCIENCES OF SHENYANG, SHENYANG 110001, PRC)   

  • Online:2000-06-25 Published:2000-06-28

摘要： 采用在实际工作中所遇到的脑挫伤案例标本 ,利用免疫组织化学染色 ,观察人脑挫伤后GFAP ,PCNA的改变 ,用图象分析仪对免疫组织化学染色阳性细胞的面积和灰度等进行定量测量 ,EpiInfo统计软件分析 ,得出人脑挫伤后GFAP ,PCNA变化的时间性规律 ,为脑挫伤形成时间推断 ,提供新的形态学依据。GFAP阳性细胞面积、灰度伤后24h即有显著增加 (P<0 01) ,7天达高峰 (P<0 01) ,以后有逐渐下降的趋势 ;PCNA阳性细胞面积、灰度于伤后4天达到最大值 (P<0 01) ,以后逐渐下降。因此 ,伤后人脑组织GFAP、PCNA免疫组织化学染色阳性细胞面积、灰度改变有时间规律 ,GFAP、PCNA可作为2天到20天间不同时间脑损伤的推断指标。

关键词: 脑挫伤, 免疫组织化学, 图象分析

Abstract: In order to find out changes of GFAP and PCNA after human brain trauma, samples of 38 cases were studied by the immunnohistochemistry method combined with quantitative image analysis The gray degree and area of PCNA positive cells increased to the maximum at the 4th day and then decreased gradually GFAP positive cells began to increase significantly 12 hours after contusion, reached the maximum at the 4th day and stayed in high level until the 7th day These results suggest that: (1) GFAP, PCNA show regular changes with survival time after brain contusion;(2) GFAP and PCNA can be used as time markers of brain contusion from 2-20 days

Key words: brain trauma, immunnohistochemistry, image analysis (Original article on page 137)