法医学杂志

法医学杂志 ›› 2000, Vol. 0 ›› Issue (3): 143-145+.

• 论文 • 上一篇    下一篇

STR和性别位点PCR产物的荧光自动检测

江斌,梁赏猷,郭景元   

  1. 衡阳医学院法医物证鉴定中心!湖南421001,广州市刑事科学技术研究所!广东510030,中山医科大学法医系!广东广州510089
  • 发布日期:2000-06-25 出版日期:2000-06-28

Automated fluorescent analysis of STR profiling and sex determination

JIANG BIN, ET AL (LEGAL MEDICAL EXPERTISE CENTER OF HENGYANG MEDICAL COLLEGE, HENGYANG 421001, PRC)   

  • Online:2000-06-25 Published:2000-06-28

摘要： 运用荧光染料标记引物、变性聚丙烯酰胺凝胶电泳 (denaturingpolyacrylamidegelelec trophoresis,denaturingPAGE)结合荧光法检测6个STR位点 (vWA31A、TH01、F13A1、FES、TPOX、CSF1PO)及1个性别鉴定位点(Amelogenin) ,就荧光自动检测的重复性、准确性、分辩力等方面进行了研究 ,建立了6个STR位点等位基因判定视窗及用荧光DNA测序仪进行STR分析的方法 ,实现电脑自动判读结果。结果表明 ,本文所述STR和性别鉴定的自动检测方法可靠、分辨力高 (可达1bp) ,所得数据有助于荧光自动DNA分型软件系统的数据积累。

关键词: 荧光DNA自动测序仪, STR分型, 性别预选, 人工假象

Abstract: Denaturing PAGE coupled with the ABI377 fluorescent automated DNA sequencer was used to test the performance and reproducibility of the automated DNA profiling systems at vWA31A, TH01, F13A01, FES, TPOX, CSF1PO and Amelogenin gene The allele designation windows at the 7 genetic markers were established and implemented into the genotype reading software Alleles differing in just 1bp in length could easily be discriminated Furthermore, the interpretation guidelines were outlined for the 7 genetic systems by investigating the relative peak areas of heterozygote peaks and relative stutter peak areas in various monoplex systems Our results indicate that if the ratio between two peaks is equal to or higher than 0 404, a herozygote could be determined, otherwise the homozygote be made

Key words: Automated fluorescent DNA sequencer, STR profiling, sex preselection, stutter bands