法医学杂志

法医学杂志 ›› 2000, Vol. 0 ›› Issue (4): 211-213+.

• 论文 • 上一篇    下一篇

大鼠脑挫伤后组织学及Bax/Bcl-2表达的研究

陈龙,陈忆九,李中   

  1. 复旦大学基础医学院法医学教研室!上海 200032,司法部司法鉴定科学技术研究所!上海 200063 ,复旦大学基础医学院法医学教研室!上海 200032
  • 发布日期:2000-08-25 出版日期:2000-08-28

Study on histology and the expression of Bax and Bcl－ 2 after cerebral contusion in rats

CHEN LONG,ET AL.(DEPARTMENT OF FORENSIC MEDICINE, MEDICAL CENTER OF FUDAN UNIVERSITY SHANGHAI 200032;)   

  • Online:2000-08-25 Published:2000-08-28

被引次数

21

摘要： 采用自制大鼠右顶叶局灶性脑挫伤模型，进行组织学和 Bax/Bcl－ 2的免疫组织化学法研究。结果发现 :伤后 12h～ 24h，挫伤灶周围神经细胞和星形胶质细胞形态学发生改变，白细胞附壁和游出；伤后 4d，挫伤灶周围出现大量的泡沫细胞和核大而深染的星形胶质细胞； 8～ 10d，挫伤灶处形成软化灶； 12～ 14d，挫伤灶愈合变成胶质细胞结节，或者变成囊状，可见大量的星型胶质细胞及新生毛细血管，可见含铁血黄素颗粒沉积。 Bax/Bcl－ 2表达水平与伤后经历时间有一定相关性。

关键词: 脑挫伤, 损伤时间, 凋亡, Bax, Bcl-2, 免疫组织化学, 组织学

Abstract: To set up the experimental model of focal cerebral contusions in rats,and to study the histology and the expression of Bax and Bcl－ 2 after cerebral catusions.It was found that neurons and astrocytes around the wound area were changed morphologically and that polymorphonuclear leucocytes were present at 12－ 24h after injury. A lot of foamy cells and astrocytes having a big dark nucleus appeared around the wound area on the 4th day after injury. On the 8－ 10th day, liquefaction necrosis was seen in areas of contusions. On the 12－ 14th day, the contusion focus healed up and became cystiform or glial node. Meantime, lots of astrocytes, capillary vessel and hemosiderin were observed. The expression of Bax and Bcl－ 2 increased after injury. There existed a relationship between increased Bax/Bcl－ 2 and different intervals after brain injury.

Key words: cerebral contusion, timing of injury, apoptosis, bax, bcl－ 2, immunohistochemistry, histology