法医学杂志

法医学杂志 ›› 2002, Vol. 0 ›› Issue (4): 207-209+.

• 论文 • 上一篇    下一篇

南方汉族、黎族人群15个STR基因座频率调查

杨电,刘超,彭汝标,刘长晖,潘远义   

  1. 广州市刑事科学技术研究所,广州市刑事科学技术研究所,海南省公安厅刑科所,广州市刑事科学技术研究所,海南省公安厅刑科所 广东510030 ,广东510030 ,海南海口570203 ,广东510030 ,海南海口570203
  • 发布日期:2002-08-25 出版日期:2002-08-28

Genetic studies of 15 loci in southern China Han population and Li population

YANG DIAN 1 ,LIU CHAO 1 ,PENG RU-BIAO 2 ,ET AL.(1.GUANGZHOU CRIMINAL SCIENCE&TECHNOLOGY INSTITUTE,GUANGZHOU510030;2.CRIMINAL SCIENCE&TECHNOLOGY INSTITUTE OF HAINAN PROVINCE,HAIKOU570203)   

  • Online:2002-08-25 Published:2002-08-28

被引次数

65

摘要： 目的调查南方汉族、黎族人群无关个体的15个STR基因座(D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)多态性,研究其在法医学检验中的应用价值。方法应用AmpFlSTRIdentifilerTM荧光标记复合扩增系统对南方332例汉族、334例黎族无关个体血样DNA进行15个STR基因座的复合扩增,用ABI3100遗传分析仪对扩增产物进行检测,用GeneScan、GenoTyper软件进行基因分型,统计计算15个STR基因座的群体遗传学参数。结果IdentifilerTM荧光标记系统的15个STR基因座在南方汉族、黎族人群的累积偶合率分别为4.94×10-17、2.50×10-17,累积非父排除率分别为0.9999989、0.9999988。结论该15个STR基因座足可满足南方汉族、黎族法医学的个体识别及亲权鉴定的需要。

关键词: IdentifilerTM, 复合扩增, 荧光标记, STR基因座, 遗传多态性, 法医学应用

Abstract: Objective The study was carried out on investigating genetic polymorphism of15STR loci in southern China Han population and Li population.Methods DNA samples from332unrelated individuals of southern China Han population and334individuals of Li population were screened by using AmpFlSTR Identifiler TM PCR Amplification Kit and3100Genetic Analyzer.Results These15loci met the Hardy-Weinberg expectations.The matching probability of the15STR loci was4.94 × 10 -17 ?2.50 × 10 -17 and com bined paternity of exclution was0.9999989?0.9999988in the two population.Conclusion Our results showed Identifiler TM PCR Amplification systems of15STR loci were useful to forensic case work in south ern China Han population and Li population.

Key words: identifiler TM, multiplexing, PCR, STR loci, genetic polymorphism, forensic sciecce