法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (2): 73-76+8.

• 论文 • 上一篇    下一篇

大鼠脑挫伤后NF-κB表达的分子生物学研究

陶陆阳,丁梅,陈溪萍,卞士中,官大威   

  1. 中国医科大学法医学院,中国医科大学法医学院,中国医科大学法医学院,苏州大学医学院法医学教研室,中国医科大学法医学院 辽宁沈阳110001,苏州大学医学院法医学教研室,江苏苏州215007 ,辽宁沈阳110001 ,辽宁沈阳110001,苏州大学医学院法医学教研室,江苏苏州215007 ,江苏苏州215007 ,辽宁沈阳110001
  • 发布日期:2004-04-25 出版日期:2004-04-28

The study on expression of NF-κB in experimental brain contusion in rats

TAO LU-YANG, CHEN XI-PING , DING MEI, ET AL. (SCHOOL OF MEDICINE,SUZHOU UNIVERSITY, SUZHOU 215007, CHINA)   

  • Online:2004-04-25 Published:2004-04-28

被引次数

75

摘要： 目的阐明不同程度及不同时间脑挫伤后NF-κB表达的变化。方法应用免疫组织化学染色、Westernblot和RT-PCR方法对48例不同程度实验性脑挫伤大鼠的脑组织进行了检验,同时以3例非脑挫伤和3例假手术大鼠的脑组织做对照。结果伤后1hNF-κB即有表达、24～48h达高峰,一周后停止表达。阳性细胞多集中于挫伤区及周围带皮质和脑实质内海马区;不同程度脑挫伤组之间于伤后各个时间段NF-κB表达无明显差异。结论本实验首次通过对不同程度脑损伤后NF-κB表达量的研究,找到其表达的时间性变化规律,对确定脑挫伤形成时间有意义,但不能作为判断脑挫伤程度的指标。

关键词: 脑挫伤, NF-κB, 脑损伤程度, 脑损伤时间, 凋亡

Abstract: Objective To investigate the expression of NF-κB in different post-traumatic intervals and severity of brain injury. Methods Fifty-four brain tissue samples of slight (n=24), moderate(n=24) brain injury, sham(n=3) and control (n=3) of rat were examined by immunohistochemical staining, westernblot and RT-PCR. Results Up-regulating of NF-κB expression was found in tissues from traumatic brain injury compared with controls in early 1 hour after TBI, and reached peak at 24h and 48h and disappeared after 7 days. The expression of NF-κB mRNA has association with contusion severity. Conclusion NF-κB may benefit to the estimation of posttraumatic intervals of brain injury.

Key words: cerebral contusion, NF-κB, severity, posttraumatic intervals, apoptosis