单个细胞DNA样本的制备及其在灵敏度分析中的应用研究

法医学杂志 ›› 2005, Vol. 0 ›› Issue (1): 6-8.

单个细胞DNA样本的制备及其在灵敏度分析中的应用研究

邓建强,石美森,应斌武,李英碧,吴谨,侯一平

司法部司法鉴定科学技术研究所,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院上海200063 ,四川成都610041 ,四川成都610041 ,四川成都610041 ,四川成都610041 ,四川成都610041

发布日期:2005-02-25 出版日期:2005-02-28

DNA Samples Preparation from Single Cell and its Application in Sensitivity Test

DENG JIAN-QIANG1, SHI MEI-SEN2, YING BING-WU2, ET AL. (1.INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063,CHINA; 2. INSTITUTE OF FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041 CHINA)

Online:2005-02-25 Published:2005-02-28

摘要/Abstract

摘要： 目的建立一种用于高精确灵敏度分析的微量DNA样本制备方法。方法用显微捕获技术制备以细胞为单位的DNA样本,并用于ProfilerPlus试剂盒及ABI310遗传分析仪的灵敏度测试。结果建立了单细胞捕获操作方案,成功制备了依次含1～11个细胞的一组DNA分析样本,并可准确用于ProfilerPlus誖试剂盒及ABI310遗传分析仪的灵敏度研究。结论基于显微操作技术的高精确微量DNA分析样本制备方法可准确用于分析技术方法的灵敏度研究

关键词: 灵敏度分析, DNA样本制备, 单个细胞

Abstract: Objective To establish a reliable, exact and practical method to prepare DNA samples for sensitivity-test purposes. Methods The micromanipulation method was employed to prepare exact quantity DNA samples used to study the sensitivity of Profiler Plus?誖Kit-ABI PRISMTM 310 system. Results We succeed in establishing a micromanipulation method to prepare groups of DNA samples ,which contain 1-11 cells in turn, and also succeed in using them to study the sensitivity of Profiler Plus?誖Kit-ABI 310 system. Conclusion The method we have established is proved to be a reliable, exact and practical way to prepare DNA samples for sensitivity-test purposes.

Key words: sensitivity test, DNA sample preparation, single cell

邓建强;石美森;应斌武;李英碧;吴谨;侯一平. 单个细胞DNA样本的制备及其在灵敏度分析中的应用研究[J]. 法医学杂志, 2005, 0(1): 6-8.

DENG JIAN-QIANG;SHI MEI-SEN;YING BING-WU;ET AL. (.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA;. INSTITUTE OF FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00 CHINA). DNA Samples Preparation from Single Cell and its Application in Sensitivity Test[J]. , 2005, 0(1): 6-8.