法医学杂志

法医学杂志 ›› 2005, Vol. 21 ›› Issue (1): 9-10+1.

• 论文 • 上一篇    下一篇

用复合扩增方法检测4个Y-STR基因座单倍型

刘秋玲,吕德坚,许呢妹,陆惠玲,陈丽娴,唐双伯   

  1. 中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室 广东广州510089 ,广东广州510089 ,广东广州510089 ,广东广州510089 ,广东广州510089 ,广东广州510089
  • 发布日期:2005-02-25 出版日期:2005-02-28

Detecting Haplotypes of Four Y-STR by Multiplex Amplification

LIU QIU-LING, LU DE-JIAN, XU NI-MEI, ET AL. (FAULTY OF FORENSIC MEDICINE, ZHONG SHAN MEDICAL COLLEGE , ZHONG SHAN UNIVERSITY, GUANGZHOU 510089,CHINA)   

  • Online:2005-02-25 Published:2005-02-28

被引次数

1

摘要： 目的建立复合扩增Y-STR基因座的体系,获得中国汉族人的单倍型频率。方法复合扩增DYS439、DYS390、GATA-A7.2和DYS3934个基因座,用聚丙烯酰胺凝胶电泳和银染法进行基因分型。结果调查中国汉族558名无关男性个体,4个基因座分别检出7、7、7和6个等位基因,共180种单倍型,其单倍型的个体识别率为0.9853。结论该复合扩增体系在建立Y染色体STR数据库、在群体遗传研究和法医学鉴定中有应用意义。

关键词: 复合扩增, Y染色体, 短串联重复(STR), 单倍型

Abstract: Objective To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population. Methods DYS439?DYS390?GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining. Results When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439?DYS390?GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853. Conclusion The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.

Key words: multiplex amplification, Y-chromosome, short tandem repeats(STR), haplotype