大鼠弥散性轴索损伤后β-APP的表达

法医学杂志 ›› 2005, Vol. 0 ›› Issue (3): 165-168+.

大鼠弥散性轴索损伤后β-APP的表达

朱金龙,朱少华,任亮,刘良,周亦武,陈虎,邓伟年

华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,华中科技大学同济医学院法医学系,武汉市公安局汉阳分局,华中科技大学同济医学院法医学系湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030 ,湖北武汉430030 ,湖北武汉430050 ,湖北武汉430030

发布日期:2005-06-25 出版日期:2005-06-28

Expression of β- amyloid Precursor Protein in Diffuse Axonal Injury of Rats

ZHU JIN-LONG, ZHU SHAO-HUA, REN LIANG,ET AL.(DEPARTMENT OF FORENSIC MEDICINE, TONGJI MEDICAL COL-LEGE, HUAZHONG UNIVERSITY OF SCIENCE & TECHNOLOGY ,WUHAN 430030,CHINA)

Online:2005-06-25 Published:2005-06-28

摘要/Abstract

摘要： 目的观察大鼠DAI损伤后β-APP表达和Gless神经纤维轴索染色在诊断DAI损伤及判断损伤时间的价值。方法按Marmarou法复制大鼠DAI损伤模型,脑组织常规取材后进行β-APP及Gless氏神经纤维轴索染色观察。结果β-APP及Gless氏染色法在大鼠DAI损伤后0.5h即可见神经轴索断裂、扭曲变形、增粗膨大,12h以后可见到轴索收缩球。二种方法均显示DAI损伤的病理形态学变化,伤后12h明显,1d达到高峰,3d后开始修复,10d后基本恢复正常。β-APP表达强度在实验组不同时间存在着明显的差异,即3h呈明显阳性表达,1d达到高峰,3d后逐渐减弱,10d基本恢复正常。结论β-APP免疫组织化学染色法及Gless氏神经纤维轴索染色法,对DAI的早期诊断具有重要应用价值,并能从病理形态学上反映DAI损伤的时序性。β-APP表达强度变化是推断早期DAI损伤时间的重要参考指标。

关键词: 弥散性轴索损伤, β-APP, 免疫组织化学染色, 损伤时间推断

Abstract: Objective To explore an method for diffuse axonal injury (DAI) diagnosis and injury time esti-mation, the changes of β- APP immunoreactivity and to observe the morphology of axonal in different partsof brain after experimental DAI injury. Methods The animal models of DAI was established according tothe Marmarou′s method. Immunohistochemistry and Gless staining were performed to observe the changesof β- APP expression and the morphology of axon with the time elapsed after the DAI injury. Results Inthe brain injury group, the morphologic changes of axon in brain stem were showed as twisted,broken andswellen at 0.5h , and the myelin sheaths broken could be observed, the retraction ball was found at 12h. Those morphology changes further progressed at 12h, reached to peack up to 1d, then repaired at 3d,and recovered at 10d; Meanwhile the analysis of β- APP immunoreactivity was also showed a time- depen-dent difference as fellows: β- APP expression begin at 3h, increased its immunoreactivity at 12h, reachedto maximize at 1 d, decreased after 3 d, returned to basal level at 10 d. Conclusions The results suggestthat β- APP immunohistochemistry combine with Gless staining be sensitive methods for DAI diagnosis,they could discover the time- dependent changes of the axonal morphology.The changes β- APP are quiteregular and could be used for timing DAI injury.

Key words: diffuse axonal injury, β- amyloid precursor protein, immunohistochemistry, injury time estimation

朱金龙;朱少华;任亮;刘良;周亦武;陈虎;邓伟年. 大鼠弥散性轴索损伤后β-APP的表达[J]. 法医学杂志, 2005, 0(3): 165-168+.

ZHU JIN-LONG;ZHU SHAO-HUA;REN LIANG;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COL-LEGE;HUAZHONG UNIVERSITY OF SCIENCE & TECHNOLOGY;WUHAN 000;CHINA). Expression of β- amyloid Precursor Protein in Diffuse Axonal Injury of Rats[J]. , 2005, 0(3): 165-168+.

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