法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (2): 154-159.DOI: 10.12116/j.issn.1004-5619.2019.02.005

所属专题： 损伤时间推断

• 专题 • 上一篇    下一篇

大鼠骨骼肌挫伤后TGF-β1及EⅢA-FN的表达与损伤时间推断

刘冉1，2， 葛鲁邹3， 张海东1，2， 赵东1，2， 侯伟良1，2， 鄂晓霏1，2， 于天水1，2   

  1. 1. 司法文明协同创新中心，北京 100088； 2. 中国政法大学 证据科学教育部重点实验室，北京 100192； 3. 威海市公安局高区分局，山东 威海 264200
  • 发布日期:2019-04-25 出版日期:2019-04-28
  • 通讯作者: 于天水，男，博士，副主任法医师，副教授，硕士研究生导师，主要从事法医病理学教学、科研及鉴定；E-mail：yutianshui-2000@qq.com
  • 作者简介:刘冉（1988—），男，主检法医师，主要从事法医病理学鉴定及研究；E-mail：raul_17@sina.com
  • 基金资助:
    中国政法大学校级科学研究青年资助项目（10817336）

Expressions of TGF-β1 and EⅢA-FN after Rat Skeletal Muscle Contusion and Wound Age Estimation

LIU Ran1,2, GE Lu-zou3, ZHANG Hai-dong1,2, ZHAO Dong1,2, HOU Wei-liang1,2, E Xiao-fei1,2, YU Tian-shui1,2   

  1. 1. Collaborative Innovation Center of Judicial Civilization, Beijing 100088, China; 2. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100192, China;3. High District Branch of Weihai Public Security Bureau, Weihai 264200, Shandong Province, China
  • Online:2019-04-25 Published:2019-04-28

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摘要： 目的 研究大鼠骨骼肌生前损伤、生前损伤死后以及死后损伤不同时间点转化生长因子β1（transforming growth factor β1，TGF-β1）及纤连蛋白EⅢA片段（EⅢA-fibronectin，EⅢA-FN）的表达情况，探讨其在损伤时间推断上的应用价值。 方法 建立大鼠骨骼肌挫伤模型，随机分为正常对照组（5只）、生前挫伤组（40只）、生前挫伤死后表达组（110只）和死后挫伤组（25只）。应用免疫组织化学染色检测生前挫伤组大鼠骨骼肌中TGF-β1及EⅢA-FN的蛋白表达情况，同时应用实时荧光定量PCR技术检测各组TGF-β1及EⅢA-FN mRNA的表达变化。 结果 免疫组织化学染色结果显示：生前挫伤后12 h~14 d，损伤区内大量多形核白细胞、单个核细胞和成纤维样细胞强表达TGF-β1；伤后3~7 d，EⅢA-FN主要分布于细胞外基质。实时荧光定量PCR检测结果显示：生前挫伤组TGF-β1和EⅢA-FN mRNA的表达分别于伤后3 d和5 d达到峰值；生前挫伤死后表达组大鼠TGF-β1和EⅢA-FN mRNA的表达分别于死后6 h和12 h达到峰值；死后挫伤组中死后0.5~12 h致伤的大鼠TGF-β1和EⅢA-FN mRNA表达明显低于正常对照组及生前挫伤组。 结论 TGF-β1及EⅢA-FN有望成为骨骼肌损伤时间推断的参考指标。

关键词: 法医病理学, 创伤和损伤, 骨骼肌, 转化生长因子&beta, 1, 纤连蛋白, EⅢA片段, 损伤时间推断, 大鼠

Abstract: Objective To study the expressions of transforming growth factor-β1 （TGF-β1） and EⅢA-fibronectin （EⅢA-FN） at different time points of antemortem injury, antemortem injury postmortem expression and postmortem injury and to explore their application value in wound age estimation. Methods A model of rat skeletal muscle contusion was established. The rats were randomly divided into normal control group （n=5）, antemortem contusion group （n=40）, antemortem contusion postmortem expression group （n=110） and postmortem injury group （n=25）. The expressions of TGF-β1 and EⅢA-FN after rat skeletal muscles antemortem contusion were detected with immunohistochemical staining. Expression changes of TGF-β1 and EⅢA-FN mRNA in each group were analyzed with real-time fluorescence quantitative PCR. Results Immunohistochemical staining results showed that a large number of polymorphonuclear leukocyte, mononuclear cells and fibroblastic cells showed a strong expression of TGF-β1 in wounded zones 12 h-14 d after antemortem contusion. EⅢA-FN was mainly distributed in the extracellular matrix, 3 to 7 d post-traumatic. Real-time fluorescence quantitative PCR results showed that TGF-β1 and EⅢA-FN mRNA in antemortem injury group reached the peak at 3 and 5 d post-traumatic respectively. The expressions of TGF-β1 and EⅢA-FN mRNA in antemortem contusion postmortem expression group peaked at 6 h and 12 h postmortem. The expression of TGF-β1 and EⅢA-FN mRNA in postmortem injury group 0.5-12 h postmortem was significantly lower than those of the normal control group and the antemortem contusion group. Conclusion TGF-β1 and EⅢA-FN might become a reference index for skeletal muscle wound age estimation.

Key words: forensic pathology, wounds and injuries, skeletal muscle, transforming growth factor β1, fibronectin, EⅢA segment, wound age estimation, rats