法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (1): 43-44+4.

• 论文 • 上一篇    下一篇

全基因组扩增法应用于低拷贝数DNA检测

周怀谷;张晨;   

  1. 上海市刑事科学技术研究所,上海市刑事科学技术研究所 上海200083,上海200083
  • 发布日期:2006-02-25 出版日期:2006-02-28

Study on Application of the Whole Genome Amplification in LCN

ZHOU HUAI-GU, ZHANG CHEN (SHANGHAI INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY, SHANGHAI 200083,CHINA)   

  • Online:2006-02-25 Published:2006-02-28

被引次数

54

摘要： 目的建立基于多重置换扩增(MDA)技术的全基因组扩增(WGA)方法,实现对低拷贝数(LCN)DNA样品进行分析。方法采用REPLI-g试剂盒对样本进行等温全基因组扩增,扩增产物采用ProfilerPlus试剂盒确定样本的十个STR基因座的等位基因型。结果10pgDNA模板经全基因组扩增后,能够进行DNA分型。结论全基因组扩增可以用于LCN的DNA分析,帮助提高微量物证的检出成功率。

关键词: 多重置换扩增, 全基因组扩增, 低拷贝数

Abstract: Objective To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN). Methods DNA sample was used for WGA according to the REPLI-g kit protocol (QIAGEN,Germany). WGA product was used for DNA analysis according to the Applied Biosystems Profiler Plus kit protocol (ABI, USA). Results WGA product of DNA sample (10 pg) can be used for STR genotyping. Conclusion WGA technology could be helpful for LCN DNA analysis.

Key words: multiple displacement amplification, whole genome amplification, low copy number