法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (5): 361-363.DOI: 10.3969/j.issn.1004-5619.2010.05.011

• 技术与应用 • 上一篇    下一篇

浓缩DNA法结合miniSTR分型技术检验微量DNA

顾丽华，董  研，张  晨，许  炎，陈荣华，胡  伟，陈连康，周怀谷   

  1. （上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室，上海 200083）
  • 发布日期:2010-10-25 出版日期:2010-10-28
  • 通讯作者: 通信作者：周怀谷，男，博士，主任法医师，主要从事法医DNA分析技术的应用和研究；E－mail：hgzhou803@hotmail.com
  • 作者简介:顾丽华（1967—），女，浙江海盐人，主任法医师，主要从事法医DNA分析技术的应用和研究；E-mail：crislh@eastday.com
  • 基金资助:

    上海市科学技术委员会科研计划项目（072512022）

Forensic Analysis of LCN DNA Using Sample Concentration Methods Followed by miniSTR Genotyping

GU LI-HUA, DONG YAN, ZHANG CHEN, XU YAN, CHEN RONG-HUA, HU WEI, CHEN LIAN-KANG, ZHOU HUAI-GU   

  1. （Shanghai Key Laboratory of Crime Science Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China）
  • Online:2010-10-25 Published:2010-10-28

被引次数

17

摘要： 目的 优化低拷贝数DNA STR分型方法。 方法 对采用磁珠法或Chelex-100法提取DNA，Identifiler试剂盒扩增，未获得分型结果的日常检案检材，采用物理浓缩法或过柱浓缩法浓缩DNA，采用miniFilerTM试剂盒再次扩增分型。 结果 127例检材中，47例磁珠法提取DNA未获得分型的样品，分型成功率为36%；80例Chelex-100法提取DNA未获分型的样品，分型成功率为30%。 结论 采用浓缩法和miniFilerTM试剂盒，可以提高日常检案中低拷贝数检材的STR检验分型成功率。

关键词: 法医遗传学, 低拷贝数, DNA检验

Abstract: Objective To optimize low copy number（LCN） DNA analysis methods for forensic STR genotyping. Methods Two groups of DNA sample, extracted using either Magnetic bead method or Chelex-100 methods, were previously amplified with a Identifiler PCR Amplification kit, but no genotype was detected. The DNA samples were concentrated using either a drying method or the Microcon-100 method, then amplified using an miniFilerTM PCR Amplification kit and genotyped. Results Among the 127 DNA samples, 47 samples, previously extracted using the Magnetic bead method, were genotyped with 36% success rate. Eighty samples, previously extracted using the Chelex-100 method, were genotyped with 30% success rate. Conclusion The application of sample concentration methods and miniFilerTM kit can improve the success rate of LCN STR analysis.

Key words: forensic genetics, low copy number, DNA examination

中图分类号: