法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (3): 213-216.

• 论文 • 上一篇    下一篇

考古样品中Amelogenin同源基因的提取和检测

张虎勤;杨周岐;刘芳娥;张金;赵文明;   

  1. 西安交通大学生物医学信息工程教育部重点实验室生命科学与技术学院,西安交通大学生物医学信息工程教育部重点实验室生命科学与技术学院,第四军医大学基础医学部教学实验中心,西安交通大学生物医学信息工程教育部重点实验室生命科学与技术学院,西安交通大学生物医学信息工程教育部重点实验室生命科学与技术学院 陕西西安710049,陕西西安710049,陕西西安710032,陕西西安710049,陕西西安710049
  • 发布日期:2006-06-25 出版日期:2006-06-28

Homologous Amelogenin Gene Test of Archaeological Samples

ZHANG HU-QIN1, YANG ZHOU-QI1, LIU FANG-E2, ZHANG JIN1, ZHAO WEN-MING1(1.THE KEY LABORATORY OF BIOMEDICAL INFORMATION ENGINEERING OF MINISTRY OF EDUCATION, SCHOOL OF LIFE SCIENCE AND TECHNOLOGY, XI′AN JIAOTONG UNIVERSITY, XI′AN 710049, CHINA; 2.THE CENTER   

  • Online:2006-06-25 Published:2006-06-28

摘要： 目的利用人类性染色体Amelogenin同源基因在X、Y染色体上序列长度的差异,选择设计引物,对考古样本进行古DNA性别信息研究。方法采用苯酚/氯仿-二氧化硅-超滤离心方法提取东岭墓葬群殉人骨骼、牙齿古DNA,PCR扩增,非变性聚丙烯酰胺凝胶电泳(PAGE)分离和检测古DNA扩增片段。结果8个墓葬16个样品中有7个样品出现阳性扩增检测,目标基因片段清楚,男性为二条带(X、Y),女性为一条带(X),牙齿样品检测成功率优于骨骼样品。结论改进的苯酚/氯仿—二氧化硅—超滤分离法是较好的古DNA提取方法,具有降低PCR抑制剂、消耗成本低和提取成功率高等特点。基于人类X、Y染色体Amelogenin同源基因的古DNA性别分析方法可成为分子考古重要的技术方法。

关键词: Amelogenin基因, 古DNA, 分子性别鉴定, 分子考古

Abstract: Objective Based on the sequence differences of Amelogenin homologous gene in the X and Y chromosomes, a pair of specific primers was designed to identify the sex of archaeological samples. Methods Ancient DNA fragments were extracted from the bones and teeth of sacrificial slaves with an improved method that combines phenol-chloroform extraction, silicon dioxide adsorption with ultrafiltration concentration. The polyacrylamide gel electrophoresis (PAGE) was used to detect PCR products. Results Seven in sixteen samples from eight graves showed positive results and the targeted segments were visible: a male with two bands of 106 bp (Amel-X) and 112 bp (Amel-Y), while a female with only one band of 106 bp (Amel-X). Ancient DNA analyzing results from tooth samples are more marked than that from bones. Conclusion The improved extraction method is more effective for ancient DNA extraction, which reduced the PCR inhibitors and lowered experimental costs. The sex determination technology based on Amelogenin homologous gene is an important and feasible method in the molecular archaeological research.

Key words: amelogenin gene, ancient DNA, molecular sex identification, molecular archaeology