法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (3): 193-195.DOI: 10.3969/j.issn.1004-5619.2016.03.008

• 技术与应用 • 上一篇    下一篇

PowerPlex?誖21试剂盒扩增后的分型图谱中Y片段丢失的识别

汪三存1，丁美满1，魏晓林1，张  涛2，姚  斐1   

  1. （1. 嘉兴市公安局刑侦支队DNA实验室，浙江 嘉兴 314001； 2. 嘉兴市公安局南湖区分局刑侦大队，浙江 嘉兴 314000）
  • 发布日期:2016-06-25 出版日期:2016-06-28
  • 作者简介:汪三存（1976—），男，主检法医师，主要从事法医DNA研究；E-mail：wsc1976@sina.com

Recognition of Y Fragment Deletion by Genotyping Graphs after Amplified by PowerPlex?誖21 Detection Kit

WANG SAN-CUN1, DING MEI-MAN1, WEI XIAO-LIN1, ZHANG TAO2, YAO FEI1   

  1. （1. DNA Lab of Criminal Investigation Detachment, Jiaxing Public Security Bureau, Jiaxing 314001, China; 2. Criminal Investigation Team, Nanhu District Branch Bureau, Jiaxing Public Security Bureau, Jiaxing 314000, China）
  • Online:2016-06-25 Published:2016-06-28

摘要： 目的 依据分型图谱，直观、简便识别是否存在Amelogenin基因Y片段丢失的可能。 方法 采用计算分型图谱中等位基因峰高之和比值的方法，统计1 968份人员样本经PowerPlex?誖21试剂盒扩增后Amelogenin与D3S1358基因座之间的均衡性、Amelogenin X等位基因与Amelogenin Y等位基因的均衡性以及D3S1358基因座不同等位基因的均衡性情况。 结果 90.8%的女性样本Amelogenin X等位基因峰高不低于D3S1358基因座等位基因峰高和的60%，94.9%的男性样本Amelogenin X等位基因的峰高不超过D3S1358基因座等位基因峰高和的70%。 结论 PowerPlex?誖21试剂盒扩增后的分型结果中，当Amelogenin基因只检测到Amelogenin X等位基因，且Amelogenin X等位基因的峰高不及D3S1358基因座等位基因峰高之和的70%时，应考虑存在Y片段丢失的可能性。

关键词: 法医遗传学, DNA指纹法, 基因频率, Amelogenin基因

Abstract:  Objective To recognize the possibility of Y fragment deletion of Amelogenin gene intuitively and simply according to the genotyping graphs. Methods By calculating the ratio of total peak height of genotyping graphs, the statistics of equilibrium distribution between Amelogenin and D3S1358 loci, Amelogenin X-gene and Amelogenin Y-gene, and different alleles of D3S1358 loci from 1 968 individuals was analyzed after amplified by PowerPlex?誖21 detection kit. Results Sum of peak height of Amelogenin X allele was not less than 60% that of D3S1358 loci alleles in 90.8% female samples, and sum of peak height of Amelogenin X allele was not higher than 70% that of D3S1358 loci alleles in 94.9% male samples. Conclusion The result of genotyping after amplified by PowerPlex?誖21 detection kit shows that the possibility of Y fragment deletion should be considered when only Amelogenin X-gene of Amelogenin is detected and the peak height of Amelogenin X-gene is not higher than 70% of the total peak height of D3S1358 loci.

Key words: forensic genetics, DNA fingerprinting, gene frequency, Amelogenin gene

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