法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (4): 275-276.

• 论文 • 上一篇    下一篇

改良AS-PCR引物对ABO基因分型的影响

王新杰;刁立江;冯建忠;吕桂平;   

  1. 潍坊市公安局,潍坊市公安局,潍坊市公安局,山东省公安厅 山东潍坊261061,山东潍坊261061,山东潍坊261061,山东济南250001
  • 发布日期:2006-08-25 出版日期:2006-08-28

Influence of Meliorated AS-PCR Primer for ABO Genotyping

WANG XIN-JIE1, DIAO LI-JIANG1, FENG JIAN-ZHONG1, LU GUI-PING2 (1.WEIFANG PUBLIC SECURITY BUREAU, WEIFANG 261061,CHINA; 2.DEPARTMEMT OF PUBLIC SECURITY OF SHANDONG,JINAN 250001,CHINA))   

  • Online:2006-08-25 Published:2006-08-28

摘要： 目的通过改进AS-PCR引物,提高ABO基因型分型正确率。方法把引物P13′末端第5位碱基由G改为C,其分型图谱与改进前进行对比分析。结果改进AS-PCR引物后,OO型非特异产物减少,避免了将OO型误判为AO型。结论引物P13′末端第5位碱基由G改为C后,可有效防止OO型被误判为AO型。

关键词: AS-PCR, 改良引物, 3′末端, 碱基, ABO基因型

Abstract: Objective To improve the correct rate of ABO genotyping by meliorating AS-PCR primer. Methods The primerP1 was changed into primerP1′ by substituting the fifth base G for C of 3′end and the ABO genotyping results of primer P1 and P1′ was compared and analysed. Results The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer. Conclusion The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1′.

Key words: AS-PCR, meliorated primer, 3′-end, base, ABO genotyping