法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (5): 349-352.

• 论文 • 上一篇    下一篇

SPE-HPLC分析全血中37种药(毒)物

梁晨;张玉荣;金琦芸;郭幼梅;   

  1. 上海市刑事科学技术研究所,上海市刑事科学技术研究所,复旦大学药学院药物分析教研室,复旦大学药学院药物分析教研室 上海200083,上海200083,上海200032,上海200032
  • 发布日期:2006-10-25 出版日期:2006-10-28

Analysis of 37 Drugs in Whole Blood by HPLC after Solid Phase Extraction

LIANG CHEN1, ZHANG YU-RONG1, JIN QI-YUN2, GUO YOU-MEI2 (1.SHANGHAI INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY, SHANGHAI 200083, CHINA; 2.DEPARTMENT OF PHARMACEUTICAL ANALYSIS, SCHOOL OF PHARMACY, FUDAN UNIVERSITY, SHANGHAI 200032, CHINA)   

  • Online:2006-10-25 Published:2006-10-28

摘要： 目的建立全血中37种常见药(毒)物的固相萃取-高效液相色谱(SPE-HPLC)分析方法。方法以多沙普仑为内标,0.5mL全血经Oasis小柱固相萃取后,用HPLC进行分析,内源性物质不干扰测定。色谱柱采用LiChrospher!100RP-C18柱(250mm×4.0mm×5μm);二极管阵列检测器,检测波长为230nm和250nm,同时进行紫外扫描;柱温50℃。结果37种药(毒)物的绝对回收率除吗啡外均大于61.68%;日内及日间精密度均小于10%;检测限1～30ng/mL;线性相关系数在0.99798以上。结论本法快速、灵敏、重现性好,可用于实际案例中多种药(毒)物的分析。

关键词: 固相萃取, 高效液相色谱法, 全血

Abstract: Objective To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood. Methods With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher?誖 100 RP-C18 (250 mm×4.0 mm×5 μm)column kept at 50 ℃, the DAD detector was set at 230 nm and 250 nm. Results The limit of detection were 1～30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was≥0.997 98. The relative standard deviation for between-day and within-day assay were <10%. Conclusion The method is effective, simple, reliable and has been used in real cases.

Key words: SPE, HPLC, whole blood