法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (6): 401-403.

• 论文 •    下一篇

丝光绿蝇COⅡ序列分析与地理种群分布的关系

蔡继峰;兰玲梅;陈瑶清;王震侠;常云峰;梁伟波;董建国;廖志钢;   

  1. 中南大学基础医学院,中南大学基础医学院,中南大学基础医学院,四川大学华西临床医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,上海第二医科大学基础医学院,四川大学华西基础医学与法医学院 湖南长沙410013,湖南长沙410013,湖南长沙410013,四川成都610041,四川成都610041,四川成都610041,上海200025,四川成都610041
  • 发布日期:2006-12-25 出版日期:2006-12-28

Relationship between COⅡ Gene of mtDNA of Lucilia Sericata and Latitude Interval

CAI JI-FENG1, LAN LING-MEI1, CHEN YAO-QING1, WANG ZHEN-XIA2, CHANG YUN-FENG3, LIANG WEI-BO3, DONG JIAN-GUO4, LIAO ZHI-GANG3 (1.SCHOOL OF BASIC MEDICAL SCIENCES, CENTRAL SOUTH UNIVERSITY, CHANGSHA 410013, CHINA; 2.WEST CHINA SCHOOL OF CLINICAL MEDICINE, SI   

  • Online:2006-12-25 Published:2006-12-28

摘要： 目的探讨不同地理种群丝光绿蝇遗传距离与所处地理纬度差异分布的相关性,为法医学判断丝光绿蝇所属地理种群及推断死亡地点服务。方法采集于呼和浩特和成都地区2个地理种群4个丝光绿蝇,对其线粒体DNA(mtDNA)上细胞色素氧化酶亚单位Ⅱ(COⅡ)基因中635bp序列进行分析;同时通过GenBank中BLAST检索系统检索世界不同地理纬度地区的6个地理种群的6个丝光绿蝇该区域的mtDNA序列,用MEGA2.1软件包进行序列分析和构建系统发育树,并将分析所得各苍蝇间的遗传距离与地理纬度差值运用SPSS10.5统计软件进行回归和相关分析。结果8个地理种群丝光绿蝇mtDNA上COⅡ中635bp基因序列分析所得遗传距离与地理纬度差值之间存在相关性(r=0.286,P=0.034)。结论8个地理种群丝光绿蝇mtDNA上COⅡ中635bp基因序列分析所得遗传距离与所处地理纬度差异分布之间存在相关性。

关键词: 丝光绿蝇, 细胞色素氧化酶辅酶Ⅱ, 遗传距离, 地理纬度, 地理种群分布。

Abstract: Objective To deduce the region that the geographical species of Lucilia sericata come from and determine the scene of crime (SOC) based on the gene analysis of mtDNA COⅡ. Methods A 635 bp region for COⅡ of 4 Lucilia sericata (belong to 2 geographical species) were collected and sequenced, compared with the data of GenBank. A neighbour-joining tree with the Tamura and Nei model was constructed by MEGA2.1 package. The number of inherit intervals of inner-species were analyzes by Kimura′s two-parameter model and used for construction the relationships between hereditary and latitude interval by SPSS10.5 soft. Results It showed that they had the relationships between inherit and latitude interval for the 8 geographical species of Lucilia sericata for COⅡ. Conclusion This method can be the evidence deducing the region that the geographical species of Lucilia sericata come from and further to determine the scene of crime (SOC).

Key words: Lucilia sericata, COⅡ, hereditary interval, latitude interval, distributing of geographical species