法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (6): 455-458.

• 论文 • 上一篇    下一篇

福尔马林固定石蜡包埋组织中DNA提取

谭卓毅;丁梅;王保捷;   

  1. 中国医科大学法医学院法医血清教研室,中国医科大学法医学院法医血清教研室,中国医科大学法医学院法医血清教研室 辽宁沈阳110001,辽宁沈阳110001,辽宁沈阳110001
  • 发布日期:2006-12-25 出版日期:2006-12-28

DNA Extraction from Formalin Fixed and Paraffin Embedded Tissues

TAN ZHUO-YI, DING MEI (SCHOOL OF FORENSIC MEDICINE, CHINA MEDICAL UNIVERSITY, SHENYANG 110001,CHINA)   

  • Online:2006-12-25 Published:2006-12-28

被引次数

83

摘要： 由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用,使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织(FFPET)中获取高质量的DNA。近年来,众多学者的研究表明,通过改良预处理方法,优化蛋白酶K消化作用,简化DNA提取步骤,纯化DNA提取物等,可以有效提高FFPET中提取的DNA质量,为FFPET的DNA分析奠定了基础。

关键词: 福尔马林固定, 石蜡包埋组织, DNA提取

Abstract: Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).In recent years, a lot of researches indicate that the DNA extraction from FFPET can be developed by improving the pretreatment,optimizing the digestion condition of proteinase, simplizing the procedures of the DNA extraction, purifying the extracted DNA and so on, which may pave a way for popularizing FFPET in DNA analysis.

Key words: formalin fixed, paraffin embedded tissue, DNA extraction